Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level
Abstract
We probed the lipid expression dynamics of the oleaginous yeast Yarrowia Lipolytica. We observed that neutral lipid expression is sporadic. By performing single-cell analysis, we found that such noise emanates from the metabolic reaction level. Our results provide an alternative insight into the regulation and phenotypic variability of lipogenesis.
- Authors:
-
- Univ. of Idaho, Moscow, ID (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemical Engineering
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1259946
- Report Number(s):
- PNNL-SA-110702
Journal ID: ISSN 2045-2322; KP1704020
- Grant/Contract Number:
- AC05-76RL01830; SC0008744
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 5; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biophysical methods; metabolic engineering
Citation Formats
Vasdekis, A. E., Silverman, A. M., and Stephanopoulos, G. Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level. United States: N. p., 2015.
Web. doi:10.1038/srep17689.
Vasdekis, A. E., Silverman, A. M., & Stephanopoulos, G. Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level. United States. https://doi.org/10.1038/srep17689
Vasdekis, A. E., Silverman, A. M., and Stephanopoulos, G. Mon .
"Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level". United States. https://doi.org/10.1038/srep17689. https://www.osti.gov/servlets/purl/1259946.
@article{osti_1259946,
title = {Origins of cell-to-cell bioprocessing diversity and implications of the extracellular environment revealed at the single-cell level},
author = {Vasdekis, A. E. and Silverman, A. M. and Stephanopoulos, G.},
abstractNote = {We probed the lipid expression dynamics of the oleaginous yeast Yarrowia Lipolytica. We observed that neutral lipid expression is sporadic. By performing single-cell analysis, we found that such noise emanates from the metabolic reaction level. Our results provide an alternative insight into the regulation and phenotypic variability of lipogenesis.},
doi = {10.1038/srep17689},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {Mon Dec 14 00:00:00 EST 2015},
month = {Mon Dec 14 00:00:00 EST 2015}
}
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Works referencing / citing this record:
Microbial phenotypic heterogeneity in response to a metabolic toxin: continuous, dynamically shifting distribution of formaldehyde tolerance in Methylobacterium extorquens populations
journal, September 2019
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- PLOS Genetics
Microbial phenotypic heterogeneity in response to a metabolic toxin: Continuous, dynamically shifting distribution of formaldehyde tolerance in Methylobacterium extorquens populations
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Microbial phenotypic heterogeneity in response to a metabolic toxin: Continuous, dynamically shifting distribution of formaldehyde tolerance in Methylobacterium extorquens populations
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Exploiting Bioprocessing Fluctuations to Elicit the Mechanistics of De Novo Lipogenesis in Yarrowia lipolytica
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