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Title: Discovery and Characterization of Iron Sulfide and Polyphosphate Bodies Coexisting in Archaeoglobus fulgidus Cells

Abstract

Inorganic storage granules have long been recognized in bacterial and eukaryotic cells but were only recently identified in archaeal cells. Here, we report the cellular organization and chemical compositions of storage granules in the Euryarchaeon, Archaeoglobus fulgidusstrain VC16, a hyperthermophilic, anaerobic, and sulfate-reducing microorganism. Dense granules were apparent inA. fulgiduscells imaged by cryo electron microscopy (cryoEM) but not so by negative stain electron microscopy. Cryo electron tomography (cryoET) revealed that each cell contains one to several dense granules located near the cell membrane. Energy dispersive X-ray (EDX) spectroscopy and scanning transmission electron microscopy (STEM) show that, surprisingly, each cell contains not just one but often two types of granules with different elemental compositions. One type, named iron sulfide body (ISB), is composed mainly of the elements iron and sulfur plus copper; and the other one, called polyphosphate body (PPB), is composed of phosphorus and oxygen plus magnesium, calcium, and aluminum. PPBs are likely used for energy storage and/or metal sequestration/detoxification. ISBs could result from the reduction of sulfate to sulfide via anaerobic energy harvesting pathways and may be associated with energy and/or metal storage or detoxification. The exceptional ability of these archaeal cells to sequester different elements may have novelmore » bioengineering applications.« less

Authors:
 [1];  [2];  [3]; ORCiD logo [4];  [1]
  1. Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA; Electron Imaging Center for Nanomachines, California NanoSystems Institute, UCLA, Los Angeles, CA 90095, USA; The UCLA Biomedical Engineering Interdepartmental Program, UCLA, Los Angeles, CA 09905, USA
  2. Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA; Institute of Industrial Biotechnology, GC University, Lahore 54000, Pakistan
  3. Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA
  4. Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA; The UCLA-DOE Institute of Genomics and Proteomics, UCLA, Los Angeles, CA 90095, USA
Publication Date:
Research Org.:
Univ. of California, Los Angeles, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1248066
Alternate Identifier(s):
OSTI ID: 1258738
Grant/Contract Number:  
FC02-02ER63421; FG03-86ER13498
Resource Type:
Published Article
Journal Name:
Archaea
Additional Journal Information:
Journal Name: Archaea Journal Volume: 2016; Journal ID: ISSN 1472-3646
Publisher:
Hindawi
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Toso, Daniel B., Javed, Muhammad Mohsin, Czornyj, Elizabeth, Gunsalus, Robert P., and Zhou, Z. Hong. Discovery and Characterization of Iron Sulfide and Polyphosphate Bodies Coexisting in Archaeoglobus fulgidus Cells. United States: N. p., 2016. Web. doi:10.1155/2016/4706532.
Toso, Daniel B., Javed, Muhammad Mohsin, Czornyj, Elizabeth, Gunsalus, Robert P., & Zhou, Z. Hong. Discovery and Characterization of Iron Sulfide and Polyphosphate Bodies Coexisting in Archaeoglobus fulgidus Cells. United States. https://doi.org/10.1155/2016/4706532
Toso, Daniel B., Javed, Muhammad Mohsin, Czornyj, Elizabeth, Gunsalus, Robert P., and Zhou, Z. Hong. Fri . "Discovery and Characterization of Iron Sulfide and Polyphosphate Bodies Coexisting in Archaeoglobus fulgidus Cells". United States. https://doi.org/10.1155/2016/4706532.
@article{osti_1248066,
title = {Discovery and Characterization of Iron Sulfide and Polyphosphate Bodies Coexisting in Archaeoglobus fulgidus Cells},
author = {Toso, Daniel B. and Javed, Muhammad Mohsin and Czornyj, Elizabeth and Gunsalus, Robert P. and Zhou, Z. Hong},
abstractNote = {Inorganic storage granules have long been recognized in bacterial and eukaryotic cells but were only recently identified in archaeal cells. Here, we report the cellular organization and chemical compositions of storage granules in the Euryarchaeon, Archaeoglobus fulgidusstrain VC16, a hyperthermophilic, anaerobic, and sulfate-reducing microorganism. Dense granules were apparent inA. fulgiduscells imaged by cryo electron microscopy (cryoEM) but not so by negative stain electron microscopy. Cryo electron tomography (cryoET) revealed that each cell contains one to several dense granules located near the cell membrane. Energy dispersive X-ray (EDX) spectroscopy and scanning transmission electron microscopy (STEM) show that, surprisingly, each cell contains not just one but often two types of granules with different elemental compositions. One type, named iron sulfide body (ISB), is composed mainly of the elements iron and sulfur plus copper; and the other one, called polyphosphate body (PPB), is composed of phosphorus and oxygen plus magnesium, calcium, and aluminum. PPBs are likely used for energy storage and/or metal sequestration/detoxification. ISBs could result from the reduction of sulfate to sulfide via anaerobic energy harvesting pathways and may be associated with energy and/or metal storage or detoxification. The exceptional ability of these archaeal cells to sequester different elements may have novel bioengineering applications.},
doi = {10.1155/2016/4706532},
journal = {Archaea},
number = ,
volume = 2016,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 2016},
month = {Fri Jan 01 00:00:00 EST 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1155/2016/4706532

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Cited by: 6 works
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