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Title: Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA

Abstract

Recently, we showed the adenovirus proteinase interacts productively with its protein substrates in vitro and in vivo in nascent virus particles via one-dimensional diffusion along the viral DNA. The mechanism by which this occurs has heretofore been unknown. We show sliding of these proteins along DNA occurs on a new vehicle in molecular biology, a ‘molecular sled’ named pVIc. This 11-amino acid viral peptide binds to DNA independent of sequence. pVIc slides on DNA, exhibiting the fastest one-dimensional diffusion constant, 26±1.8 × 106 (bp)2s-1. pVIc is a ‘molecular sled,’ because it can slide heterologous cargos along DNA, for example, a streptavidin tetramer. Similar peptides, for example, from the C terminus of β-actin or NLSIII of the p53 protein, slide along DNA. Finally, characteristics of the ‘molecular sled’ in its milieu (virion, nucleus) have implications for how proteins in the nucleus of cells interact and imply a new form of biochemistry, one-dimensional biochemistry.

Authors:
 [1];  [1];  [2];  [1];  [2]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Broad Institute of MIT and Harvard, Cambridge, MA (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1258647
Grant/Contract Number:  
R01AI41599; R21AI113565
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mangel, Walter F., McGrath, William J., Xiong, Kan, Graziano, Vito, and Blainey, Paul C.. Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA. United States: N. p., 2016. Web. https://doi.org/10.1038/ncomms10202.
Mangel, Walter F., McGrath, William J., Xiong, Kan, Graziano, Vito, & Blainey, Paul C.. Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA. United States. https://doi.org/10.1038/ncomms10202
Mangel, Walter F., McGrath, William J., Xiong, Kan, Graziano, Vito, and Blainey, Paul C.. Tue . "Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA". United States. https://doi.org/10.1038/ncomms10202. https://www.osti.gov/servlets/purl/1258647.
@article{osti_1258647,
title = {Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA},
author = {Mangel, Walter F. and McGrath, William J. and Xiong, Kan and Graziano, Vito and Blainey, Paul C.},
abstractNote = {Recently, we showed the adenovirus proteinase interacts productively with its protein substrates in vitro and in vivo in nascent virus particles via one-dimensional diffusion along the viral DNA. The mechanism by which this occurs has heretofore been unknown. We show sliding of these proteins along DNA occurs on a new vehicle in molecular biology, a ‘molecular sled’ named pVIc. This 11-amino acid viral peptide binds to DNA independent of sequence. pVIc slides on DNA, exhibiting the fastest one-dimensional diffusion constant, 26±1.8 × 106 (bp)2s-1. pVIc is a ‘molecular sled,’ because it can slide heterologous cargos along DNA, for example, a streptavidin tetramer. Similar peptides, for example, from the C terminus of β-actin or NLSIII of the p53 protein, slide along DNA. Finally, characteristics of the ‘molecular sled’ in its milieu (virion, nucleus) have implications for how proteins in the nucleus of cells interact and imply a new form of biochemistry, one-dimensional biochemistry.},
doi = {10.1038/ncomms10202},
journal = {Nature Communications},
number = ,
volume = 7,
place = {United States},
year = {2016},
month = {2}
}

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    Works referencing / citing this record:

    Catalytic transport of molecular cargo using diffusive binding along a polymer track
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