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Title: Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate

Abstract

Traditional cheeses harbour complex microbial consortia that play an important role in shaping typical sensorial properties. However, the microbial metabolism is considered difficult to control. Microbial community succession and the related gene expression were analysed during ripening of a traditional Italian cheese, identifying parameters that could be modified to accelerate ripening. Afterwards, we modulated ripening conditions and observed consistent changes in microbial community structure and function. We provide concrete evidence of the essential contribution of non-starter lactic acid bacteria in ripening-related activities. An increase in the ripening temperature promoted the expression of genes related to proteolysis, lipolysis and amino acid/lipid catabolism and significantly increases the cheese maturation rate. Moreover, temperature-promoted microbial metabolisms were consistent with the metabolomic profiles of proteins and volatile organic compounds in the cheese. Finally, the results clearly indicate how processing-driven microbiome responses can be modulated in order to optimize production efficiency and product quality.

Authors:
 [1];  [1];  [1];  [2];  [1]
  1. Univ. of Naples Federico II (Italy)
  2. Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of Chicago, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1258637
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

De Filippis, Francesca, Genovese, Alessandro, Ferranti, Pasquale, Gilbert, Jack A., and Ercolini, Danilo. Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate. United States: N. p., 2016. Web. doi:10.1038/srep21871.
De Filippis, Francesca, Genovese, Alessandro, Ferranti, Pasquale, Gilbert, Jack A., & Ercolini, Danilo. Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate. United States. doi:10.1038/srep21871.
De Filippis, Francesca, Genovese, Alessandro, Ferranti, Pasquale, Gilbert, Jack A., and Ercolini, Danilo. Thu . "Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate". United States. doi:10.1038/srep21871. https://www.osti.gov/servlets/purl/1258637.
@article{osti_1258637,
title = {Metatranscriptomics reveals temperature-driven functional changes in microbiome impacting cheese maturation rate},
author = {De Filippis, Francesca and Genovese, Alessandro and Ferranti, Pasquale and Gilbert, Jack A. and Ercolini, Danilo},
abstractNote = {Traditional cheeses harbour complex microbial consortia that play an important role in shaping typical sensorial properties. However, the microbial metabolism is considered difficult to control. Microbial community succession and the related gene expression were analysed during ripening of a traditional Italian cheese, identifying parameters that could be modified to accelerate ripening. Afterwards, we modulated ripening conditions and observed consistent changes in microbial community structure and function. We provide concrete evidence of the essential contribution of non-starter lactic acid bacteria in ripening-related activities. An increase in the ripening temperature promoted the expression of genes related to proteolysis, lipolysis and amino acid/lipid catabolism and significantly increases the cheese maturation rate. Moreover, temperature-promoted microbial metabolisms were consistent with the metabolomic profiles of proteins and volatile organic compounds in the cheese. Finally, the results clearly indicate how processing-driven microbiome responses can be modulated in order to optimize production efficiency and product quality.},
doi = {10.1038/srep21871},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {2016},
month = {2}
}

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