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Title: Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser

Abstract

The assessment of wildlife health has been enhanced by the ability of point-of-care (POC) blood analysers to provide biochemical analyses of non-domesticated animals in the field. However, environmental limitations (e.g. temperature, atmospheric humidity and rain) and lack of reference values may inhibit researchers from using such a device with certain wildlife species. Evaluating the use of alternative sample types, such as plasma, in a POC device may afford researchers the opportunity to delay sample analysis and the ability to use banked samples. In this study, we examined fresh whole blood, fresh plasma and frozen plasma (sample type) pH, partial pressure of carbon dioxide (PCO2), bicarbonate (HCO3₋), total carbon dioxide (TCO2), base excess (BE), partial pressure of oxygen (PO2), oxygen saturation (sO2) and lactate concentrations in 23 juvenile American alligators (Alligator mississippiensis) using an i-STAT CG4+ cartridge. Our results indicate that sample type had no effect on lactate concentration values (F2,65 = 0.37, P = 0.963), suggesting that the i-STAT analyser can be used reliably to quantify lactate concentrations in fresh and frozen plasma samples. In contrast, the other seven blood parameters measured by the CG4+ cartridge were significantly affected by sample type. In conclusion, we were able to collect bloodmore » samples from all alligators within 2 min of capture to establish preliminary reference ranges for juvenile alligators based on values obtained using fresh whole blood.« less

Authors:
 [1];  [2];  [3];  [3]
+ Show Author Affiliations
  1. Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL); Univ. of Georgia, Athens, GA (United States). Warnell School of Forestry and Natural Resources
  2. Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL); Auburn Univ., AL (United States)
  3. Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)
Publication Date:
Research Org.:
Savannah River Ecology Laboratory (SREL), Aiken, SC (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1258028
Grant/Contract Number:  
FC09-07SR22506
Resource Type:
Accepted Manuscript
Journal Name:
Conservation Physiology
Additional Journal Information:
Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2051-1434
Publisher:
Oxford University Press and Society for Experimental Biology
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; American alligator; biochemistry; i-STAT; lactate; plasma; point-of-care

Citation Formats

Hamilton, Matthew T., Finger, John W., Winzeler, Megan E., and Tuberville, Tracey D. Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser. United States: N. p., 2016. Web. doi:10.1093/conphys/cov065.
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Hamilton, Matthew T., Finger, John W., Winzeler, Megan E., & Tuberville, Tracey D. Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser. United States. https://doi.org/10.1093/conphys/cov065
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Hamilton, Matthew T., Finger, John W., Winzeler, Megan E., and Tuberville, Tracey D. Fri . "Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser". United States. https://doi.org/10.1093/conphys/cov065. https://www.osti.gov/servlets/purl/1258028.
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@article{osti_1258028,
title = {Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser},
author = {Hamilton, Matthew T. and Finger, John W. and Winzeler, Megan E. and Tuberville, Tracey D.},
abstractNote = {The assessment of wildlife health has been enhanced by the ability of point-of-care (POC) blood analysers to provide biochemical analyses of non-domesticated animals in the field. However, environmental limitations (e.g. temperature, atmospheric humidity and rain) and lack of reference values may inhibit researchers from using such a device with certain wildlife species. Evaluating the use of alternative sample types, such as plasma, in a POC device may afford researchers the opportunity to delay sample analysis and the ability to use banked samples. In this study, we examined fresh whole blood, fresh plasma and frozen plasma (sample type) pH, partial pressure of carbon dioxide (PCO2), bicarbonate (HCO3₋), total carbon dioxide (TCO2), base excess (BE), partial pressure of oxygen (PO2), oxygen saturation (sO2) and lactate concentrations in 23 juvenile American alligators (Alligator mississippiensis) using an i-STAT CG4+ cartridge. Our results indicate that sample type had no effect on lactate concentration values (F2,65 = 0.37, P = 0.963), suggesting that the i-STAT analyser can be used reliably to quantify lactate concentrations in fresh and frozen plasma samples. In contrast, the other seven blood parameters measured by the CG4+ cartridge were significantly affected by sample type. In conclusion, we were able to collect blood samples from all alligators within 2 min of capture to establish preliminary reference ranges for juvenile alligators based on values obtained using fresh whole blood.},
doi = {10.1093/conphys/cov065},
journal = {Conservation Physiology},
number = 1,
volume = 4,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 2016},
month = {Fri Jan 01 00:00:00 EST 2016}
}
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https://doi.org/10.1093/conphys/cov065
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