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Title: Characterization of key triacylglycerol biosynthesis processes in rhodococci

Abstract

In this study, oleaginous microorganisms have considerable potential for biofuel and commodity chemical production. Under nitrogen-limitation, Rhodococcus jostii RHA1 grown on benzoate, an analog of lignin depolymerization products, accumulated triacylglycerols (TAGs) to 55% of its dry weight during transition to stationary phase, with the predominant fatty acids being C16:0 and C17:0. Transcriptomic analyses of RHA1 grown under conditions of N-limitation and N-excess revealed 1,826 dysregulated genes. Genes whose transcripts were more abundant under N-limitation included those involved in ammonium assimilation, benzoate catabolism, fatty acid biosynthesis and the methylmalonyl-CoA pathway. Of the 16 atf genes potentially encoding diacylglycerol O-acyltransferases, atf8 transcripts were the most abundant during N-limitation (~50-fold more abundant than during N-excess). Consistent with Atf8 being a physiological determinant of TAG accumulation, a Δatf8 mutant accumulated 70% less TAG than wild-type RHA1 while atf8 overexpression increased TAG accumulation 20%. Genes encoding type-2 phosphatidic acid phosphatases were not significantly expressed. By contrast, three genes potentially encoding phosphatases of the haloacid dehalogenase superfamily and that cluster with, or are fused with other Kennedy pathway genes were dysregulated. Overall, these findings advance our understanding of TAG metabolism in mycolic acid-containing bacteria and provide a framework to engineer strains for increased TAG production.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of British Columbia, Vancouver, BC (Canada)
Publication Date:
Research Org.:
Univ. of British Columbia, Vancouver, BC (Canada)
Sponsoring Org.:
USDOE
OSTI Identifier:
1257758
Grant/Contract Number:  
EE0006112
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bacterial physiology; lipids; metabolic engineering

Citation Formats

Amara, Sawsan, Seghezzi, Nicolas, Otani, Hiroshi, Diaz-Salazar, Carlos, Liu, Jie, and Eltis, Lindsay D. Characterization of key triacylglycerol biosynthesis processes in rhodococci. United States: N. p., 2016. Web. doi:10.1038/srep24985.
Amara, Sawsan, Seghezzi, Nicolas, Otani, Hiroshi, Diaz-Salazar, Carlos, Liu, Jie, & Eltis, Lindsay D. Characterization of key triacylglycerol biosynthesis processes in rhodococci. United States. doi:10.1038/srep24985.
Amara, Sawsan, Seghezzi, Nicolas, Otani, Hiroshi, Diaz-Salazar, Carlos, Liu, Jie, and Eltis, Lindsay D. Fri . "Characterization of key triacylglycerol biosynthesis processes in rhodococci". United States. doi:10.1038/srep24985. https://www.osti.gov/servlets/purl/1257758.
@article{osti_1257758,
title = {Characterization of key triacylglycerol biosynthesis processes in rhodococci},
author = {Amara, Sawsan and Seghezzi, Nicolas and Otani, Hiroshi and Diaz-Salazar, Carlos and Liu, Jie and Eltis, Lindsay D.},
abstractNote = {In this study, oleaginous microorganisms have considerable potential for biofuel and commodity chemical production. Under nitrogen-limitation, Rhodococcus jostii RHA1 grown on benzoate, an analog of lignin depolymerization products, accumulated triacylglycerols (TAGs) to 55% of its dry weight during transition to stationary phase, with the predominant fatty acids being C16:0 and C17:0. Transcriptomic analyses of RHA1 grown under conditions of N-limitation and N-excess revealed 1,826 dysregulated genes. Genes whose transcripts were more abundant under N-limitation included those involved in ammonium assimilation, benzoate catabolism, fatty acid biosynthesis and the methylmalonyl-CoA pathway. Of the 16 atf genes potentially encoding diacylglycerol O-acyltransferases, atf8 transcripts were the most abundant during N-limitation (~50-fold more abundant than during N-excess). Consistent with Atf8 being a physiological determinant of TAG accumulation, a Δatf8 mutant accumulated 70% less TAG than wild-type RHA1 while atf8 overexpression increased TAG accumulation 20%. Genes encoding type-2 phosphatidic acid phosphatases were not significantly expressed. By contrast, three genes potentially encoding phosphatases of the haloacid dehalogenase superfamily and that cluster with, or are fused with other Kennedy pathway genes were dysregulated. Overall, these findings advance our understanding of TAG metabolism in mycolic acid-containing bacteria and provide a framework to engineer strains for increased TAG production.},
doi = {10.1038/srep24985},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {2016},
month = {4}
}

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    Works referencing / citing this record:

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