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Title: Beyond the Western front: Targeted proteomics and organelle abundance profiling

Abstract

The application of westerns or immunoblotting techniques for assessing the composition, dynamics, and purity of protein extracts from plant material has become common practice. While the approach is reproducible, can be readily applied and is generally considered robust, the field of plant science suffers from a lack of antibody variety against plant proteins. The development of approaches that employ mass spectrometry to enable both relative and absolute quantification of many hundreds of proteins in a single sample from a single analysis provides a mechanism to overcome the expensive impediment in having to develop antibodies in plant science. Here, we consider it an opportune moment to consider and better develop the adoption of multiple reaction monitoring (MRM)-based analyses in plant biochemistry.

Authors:
 [1];  [2]
+ Show Author Affiliations
  1. Univ. of Copenhagen, Frederiksberg (Denmark)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of Melbourne, Melbourne, VIC (Australia)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1257256
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; multiple reaction monitoring (MRM); organelle abundance; immunoblotting; Arabidopsis; quantitative proteomics; proteomics

Citation Formats

Parsons, Harriet T., and Heazlewood, Joshua L. Beyond the Western front: Targeted proteomics and organelle abundance profiling. United States: N. p., 2015. Web. doi:10.3389/fpls.2015.00301.
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Parsons, Harriet T., & Heazlewood, Joshua L. Beyond the Western front: Targeted proteomics and organelle abundance profiling. United States. https://doi.org/10.3389/fpls.2015.00301
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Parsons, Harriet T., and Heazlewood, Joshua L. Tue . "Beyond the Western front: Targeted proteomics and organelle abundance profiling". United States. https://doi.org/10.3389/fpls.2015.00301. https://www.osti.gov/servlets/purl/1257256.
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@article{osti_1257256,
title = {Beyond the Western front: Targeted proteomics and organelle abundance profiling},
author = {Parsons, Harriet T. and Heazlewood, Joshua L.},
abstractNote = {The application of westerns or immunoblotting techniques for assessing the composition, dynamics, and purity of protein extracts from plant material has become common practice. While the approach is reproducible, can be readily applied and is generally considered robust, the field of plant science suffers from a lack of antibody variety against plant proteins. The development of approaches that employ mass spectrometry to enable both relative and absolute quantification of many hundreds of proteins in a single sample from a single analysis provides a mechanism to overcome the expensive impediment in having to develop antibodies in plant science. Here, we consider it an opportune moment to consider and better develop the adoption of multiple reaction monitoring (MRM)-based analyses in plant biochemistry.},
doi = {10.3389/fpls.2015.00301},
journal = {Frontiers in Plant Science},
number = ,
volume = 6,
place = {United States},
year = {Tue May 05 00:00:00 EDT 2015},
month = {Tue May 05 00:00:00 EDT 2015}
}
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Publisher's Version of Record
https://doi.org/10.3389/fpls.2015.00301
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