The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20
Abstract
Sulfate-reducing bacteria play major roles in the global carbon and sulfur cycles, but it remains unclear how reducing sulfate yields energy. To determine the genetic basis of energy conservation, we measured the fitness of thousands of pooled mutants of Desulfovibrio alaskensis G20 during growth in 12 different combinations of electron donors and acceptors. We show that ion pumping by the ferredoxin:NADH oxidoreductase Rnf is required whenever substrate-level phosphorylation is not possible. The uncharacterized complex Hdr/flox-1 (Dde_1207:13) is sometimes important alongside Rnf and may perform an electron bifurcation to generate more reduced ferredoxin from NADH to allow further ion pumping. Similarly, during the oxidation of malate or fumarate, the electron-bifurcating transhydrogenase NfnAB-2 (Dde_1250:1) is important and may generate reduced ferredoxin to allow additional ion pumping by Rnf. During formate oxidation, the periplasmic [NiFeSe] hydrogenase HysAB is required, which suggests that hydrogen forms in the periplasm, diffuses to the cytoplasm, and is used to reduce ferredoxin, thus providing a substrate for Rnf. We found that during hydrogen utilization, the transmembrane electron transport complex Tmc is important and may move electrons from the periplasm into the cytoplasmic sulfite reduction pathway. Finally, mutants of many other putative electron carriers have no clear phenotype, whichmore »
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Univ. of California, Berkeley, CA (United States)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1256960
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 5; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 58 GEOSCIENCES; energy metabolism; sulfate reducing bacteria; membrane complexes; Desulfovibrio; electron bifurcation
Citation Formats
Price, Morgan N., Ray, Jayashree, Wetmore, Kelly M., Kuehl, Jennifer V., Bauer, Stefan, Deutschbauer, Adam M., and Arkin, Adam P. The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20. United States: N. p., 2014.
Web. doi:10.3389/fmicb.2014.00577.
Price, Morgan N., Ray, Jayashree, Wetmore, Kelly M., Kuehl, Jennifer V., Bauer, Stefan, Deutschbauer, Adam M., & Arkin, Adam P. The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20. United States. https://doi.org/10.3389/fmicb.2014.00577
Price, Morgan N., Ray, Jayashree, Wetmore, Kelly M., Kuehl, Jennifer V., Bauer, Stefan, Deutschbauer, Adam M., and Arkin, Adam P. Fri .
"The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20". United States. https://doi.org/10.3389/fmicb.2014.00577. https://www.osti.gov/servlets/purl/1256960.
@article{osti_1256960,
title = {The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20},
author = {Price, Morgan N. and Ray, Jayashree and Wetmore, Kelly M. and Kuehl, Jennifer V. and Bauer, Stefan and Deutschbauer, Adam M. and Arkin, Adam P.},
abstractNote = {Sulfate-reducing bacteria play major roles in the global carbon and sulfur cycles, but it remains unclear how reducing sulfate yields energy. To determine the genetic basis of energy conservation, we measured the fitness of thousands of pooled mutants of Desulfovibrio alaskensis G20 during growth in 12 different combinations of electron donors and acceptors. We show that ion pumping by the ferredoxin:NADH oxidoreductase Rnf is required whenever substrate-level phosphorylation is not possible. The uncharacterized complex Hdr/flox-1 (Dde_1207:13) is sometimes important alongside Rnf and may perform an electron bifurcation to generate more reduced ferredoxin from NADH to allow further ion pumping. Similarly, during the oxidation of malate or fumarate, the electron-bifurcating transhydrogenase NfnAB-2 (Dde_1250:1) is important and may generate reduced ferredoxin to allow additional ion pumping by Rnf. During formate oxidation, the periplasmic [NiFeSe] hydrogenase HysAB is required, which suggests that hydrogen forms in the periplasm, diffuses to the cytoplasm, and is used to reduce ferredoxin, thus providing a substrate for Rnf. We found that during hydrogen utilization, the transmembrane electron transport complex Tmc is important and may move electrons from the periplasm into the cytoplasmic sulfite reduction pathway. Finally, mutants of many other putative electron carriers have no clear phenotype, which suggests that they are not important under our growth conditions, although we cannot rule out genetic redundancy.},
doi = {10.3389/fmicb.2014.00577},
journal = {Frontiers in Microbiology},
number = ,
volume = 5,
place = {United States},
year = {Fri Oct 31 00:00:00 EDT 2014},
month = {Fri Oct 31 00:00:00 EDT 2014}
}
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A universal TagModule collection for parallel genetic analysis of microorganisms
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Works referencing / citing this record:
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