DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: High-quality permanent draft genome sequence of Bradyrhizobium sp. Tv2a.2, a microsymbiont of Tachigali versicolor discovered in Barro Colorado Island of Panama

Abstract

Bradyrhizobiumsp. Tv2a.2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing root nodule of Tachigali versicolor collected in Barro Colorado Island of Panama. Here we describe the features of Bradyrhizobiumsp. Tv2a.2, together with high-quality permanent draft genome sequence information and annotation. The 8,496,279 bp high-quality draft genome is arranged in 87 scaffolds of 87 contigs, contains 8,109 protein-coding genes and 72 RNA-only encoding genes. In conclusion, this rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Authors:
 [1];  [2];  [3];  [3];  [4];  [3];  [3];  [3];  [5];  [6];  [7];  [1]
  1. Murdoch Univ. (Australia)
  2. Binghamton Univ., NY (United States)
  3. DOE Joint Genome Inst., Walnut Creek, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  5. King Abdulaziz Univ., Jeddah (Saudi Arabia); Jeddah Univ. (Saudi Arabia)
  6. King Abdulaziz Univ., Jeddah (Saudi Arabia)
  7. DOE Joint Genome Inst., Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1256909
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Standards in Genomic Sciences
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1944-3277
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Root-nodule bacteria; Nitrogen fixation; Symbiosis; Alphaproteobacteria; GEBA-RNB

Citation Formats

Tian, Rui, Parker, Matthew, Seshadri, Rekha, Reddy, TBK, Markowitz, Victor, Ivanova, Natalia, Pati, Amrita, Woyke, Tanja, Baeshen, Mohammed N., Baeshen, Nabih A., Kyrpides, Nikos, and Reeve, Wayne. High-quality permanent draft genome sequence of Bradyrhizobium sp. Tv2a.2, a microsymbiont of Tachigali versicolor discovered in Barro Colorado Island of Panama. United States: N. p., 2015. Web. doi:10.1186/s40793-015-0006-0.
Tian, Rui, Parker, Matthew, Seshadri, Rekha, Reddy, TBK, Markowitz, Victor, Ivanova, Natalia, Pati, Amrita, Woyke, Tanja, Baeshen, Mohammed N., Baeshen, Nabih A., Kyrpides, Nikos, & Reeve, Wayne. High-quality permanent draft genome sequence of Bradyrhizobium sp. Tv2a.2, a microsymbiont of Tachigali versicolor discovered in Barro Colorado Island of Panama. United States. https://doi.org/10.1186/s40793-015-0006-0
Tian, Rui, Parker, Matthew, Seshadri, Rekha, Reddy, TBK, Markowitz, Victor, Ivanova, Natalia, Pati, Amrita, Woyke, Tanja, Baeshen, Mohammed N., Baeshen, Nabih A., Kyrpides, Nikos, and Reeve, Wayne. Sun . "High-quality permanent draft genome sequence of Bradyrhizobium sp. Tv2a.2, a microsymbiont of Tachigali versicolor discovered in Barro Colorado Island of Panama". United States. https://doi.org/10.1186/s40793-015-0006-0. https://www.osti.gov/servlets/purl/1256909.
@article{osti_1256909,
title = {High-quality permanent draft genome sequence of Bradyrhizobium sp. Tv2a.2, a microsymbiont of Tachigali versicolor discovered in Barro Colorado Island of Panama},
author = {Tian, Rui and Parker, Matthew and Seshadri, Rekha and Reddy, TBK and Markowitz, Victor and Ivanova, Natalia and Pati, Amrita and Woyke, Tanja and Baeshen, Mohammed N. and Baeshen, Nabih A. and Kyrpides, Nikos and Reeve, Wayne},
abstractNote = {Bradyrhizobiumsp. Tv2a.2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing root nodule of Tachigali versicolor collected in Barro Colorado Island of Panama. Here we describe the features of Bradyrhizobiumsp. Tv2a.2, together with high-quality permanent draft genome sequence information and annotation. The 8,496,279 bp high-quality draft genome is arranged in 87 scaffolds of 87 contigs, contains 8,109 protein-coding genes and 72 RNA-only encoding genes. In conclusion, this rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.},
doi = {10.1186/s40793-015-0006-0},
journal = {Standards in Genomic Sciences},
number = 1,
volume = 10,
place = {United States},
year = {Sun May 17 00:00:00 EDT 2015},
month = {Sun May 17 00:00:00 EDT 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 4 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Legumes Symbioses: Absence of Nod Genes in Photosynthetic Bradyrhizobia
journal, June 2007


The DOE-JGI Standard Operating Procedure for the Annotations of Microbial Genomes
journal, July 2009

  • Mavromatis, Konstantinos; Ivanova, Natalia N.; Chen, I-Min A.
  • Standards in Genomic Sciences, Vol. 1, Issue 1
  • DOI: 10.4056/sigs.632

Bradyrhizobium iriomotense sp. nov., Isolated from a Tumor-Like Root of the Legume Entada koshunensis from Iriomote Island in Japan
journal, June 2008

  • Islam, Muhammad Saiful; Kawasaki, Hiroko; Muramatsu, Yuki
  • Bioscience, Biotechnology, and Biochemistry, Vol. 72, Issue 6
  • DOI: 10.1271/bbb.70739

Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil
journal, October 2014

  • da Silva, Krisle; De Meyer, Sofie E.; Rouws, Luc F. M.
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 64, Issue Pt_10
  • DOI: 10.1099/ijs.0.063727-0

Genome-wide identification, structural analysis and expression profiles of GRAS gene family in orchardgrass
journal, February 2020


Nodulation in Dimorphandra wilsonii Rizz. (Caesalpinioideae), a Threatened Species Native to the Brazilian Cerrado
journal, November 2012


R Factor Transfer in Rhizobium leguminosarum
journal, September 1974


SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB
journal, November 2007

  • Pruesse, E.; Quast, C.; Knittel, K.
  • Nucleic Acids Research, Vol. 35, Issue 21
  • DOI: 10.1093/nar/gkm864

A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey
journal, February 2015

  • Reeve, Wayne; Ardley, Julie; Tian, Rui
  • Standards in Genomic Sciences, Vol. 10, Issue 1
  • DOI: 10.1186/1944-3277-10-14

tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence
journal, March 1997


Prodigal: prokaryotic gene recognition and translation initiation site identification
journal, March 2010


Solexa Ltd
journal, June 2004


The Fast Changing Landscape of Sequencing Technologies and Their Impact on Microbial Genome Assemblies and Annotation
journal, December 2012


The Genomes OnLine Database (GOLD) v.4: status of genomic and metagenomic projects and their associated metadata
journal, December 2011

  • Pagani, I.; Liolios, K.; Jansson, J.
  • Nucleic Acids Research, Vol. 40, Issue D1
  • DOI: 10.1093/nar/gkr1100

IMG ER: a system for microbial genome annotation expert review and curation
journal, June 2009


Class I. Alphaproteobacteria class. nov.
book, January 2005

  • Garrity, George M.; Bell, Julia A.; Lilburn, Timothy
  • Bergey’s Manual® of Systematic Bacteriology
  • DOI: 10.1007/0-387-29298-5_1

The taxonomy of rhizobia: an overview
journal, August 2006


Mosaic origins of Bradyrhizobium legume symbionts on the Caribbean island of Guadeloupe
journal, August 2014


Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species
journal, November 2011

  • Kim, O. -S.; Cho, Y. -J.; Lee, K.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 3
  • DOI: 10.1099/ijs.0.038075-0

From North to South: A latitudinal look at legume nodulation processes
journal, November 2013


Divergent Bradyrhizobium symbionts on Tachigali versicolor from Barro Colorado Island, Panama
journal, December 2000


High-quality draft assemblies of mammalian genomes from massively parallel sequence data
journal, December 2010

  • Gnerre, S.; MacCallum, I.; Przybylski, D.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 4
  • DOI: 10.1073/pnas.1017351108

PICRUSt2 for prediction of metagenome functions
journal, June 2020

  • Douglas, Gavin M.; Maffei, Vincent J.; Zaneveld, Jesse R.
  • Nature Biotechnology, Vol. 38, Issue 6
  • DOI: 10.1038/s41587-020-0548-6

The taxonomy of rhizobia: an overview
journal, August 2006


The Spread of Bradyrhizobium Lineages Across Host Legume Clades: from Abarema to Zygia
journal, October 2014


Phylogenetic Perspectives on the Origins of Nodulation
journal, November 2011


Nodulation in Dimorphandra wilsonii Rizz. (Caesalpinioideae), a Threatened Species Native to the Brazilian Cerrado
journal, November 2012


Mosaic origins of Bradyrhizobium legume symbionts on the Caribbean island of Guadeloupe
journal, August 2014


From North to South: A latitudinal look at legume nodulation processes
journal, November 2013


Tachigalia versicolor is a suicidal neotropical tree
journal, August 1977


Legumes Symbioses: Absence of Nod Genes in Photosynthetic Bradyrhizobia
journal, June 2007


A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey
journal, February 2015

  • Reeve, Wayne; Ardley, Julie; Tian, Rui
  • Standards in Genomic Sciences, Vol. 10, Issue 1
  • DOI: 10.1186/1944-3277-10-14

Selection for acid tolerance inRhizobium meliloti
journal, September 1988

  • Howieson, J. G.; Ewing, M. A.; D'Antuono, M. F.
  • Plant and Soil, Vol. 105, Issue 2
  • DOI: 10.1007/BF02376781

R Factor Transfer in Rhizobium leguminosarum
journal, September 1974


Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species
journal, November 2011

  • Kim, O. -S.; Cho, Y. -J.; Lee, K.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 3
  • DOI: 10.1099/ijs.0.038075-0

The Genomes OnLine Database (GOLD) v.4: status of genomic and metagenomic projects and their associated metadata
journal, December 2011

  • Pagani, I.; Liolios, K.; Jansson, J.
  • Nucleic Acids Research, Vol. 40, Issue D1
  • DOI: 10.1093/nar/gkr1100

IMG 4 version of the integrated microbial genomes comparative analysis system
journal, October 2013

  • Markowitz, Victor M.; Chen, I-Min A.; Palaniappan, Krishna
  • Nucleic Acids Research, Vol. 42, Issue D1
  • DOI: 10.1093/nar/gkt963

The Fast Changing Landscape of Sequencing Technologies and Their Impact on Microbial Genome Assemblies and Annotation
journal, December 2012


Solexa Ltd
journal, June 2004


Velvet: Algorithms for de novo short read assembly using de Bruijn graphs
journal, February 2008


High-quality draft assemblies of mammalian genomes from massively parallel sequence data
journal, December 2010

  • Gnerre, S.; MacCallum, I.; Przybylski, D.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 4
  • DOI: 10.1073/pnas.1017351108

Prodigal: prokaryotic gene recognition and translation initiation site identification
journal, March 2010


The DOE-JGI Standard Operating Procedure for the Annotations of Microbial Genomes
journal, July 2009

  • Mavromatis, Konstantinos; Ivanova, Natalia N.; Chen, I-Min A.
  • Standards in Genomic Sciences, Vol. 1, Issue 1
  • DOI: 10.4056/sigs.632

Improving Microbial Genome Annotations in an Integrated Database Context
journal, February 2013


tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence
journal, March 1997


SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB
journal, November 2007

  • Pruesse, E.; Quast, C.; Knittel, K.
  • Nucleic Acids Research, Vol. 35, Issue 21
  • DOI: 10.1093/nar/gkm864

IMG ER: a system for microbial genome annotation expert review and curation
journal, June 2009


Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil
journal, October 2014

  • da Silva, Krisle; De Meyer, Sofie E.; Rouws, Luc F. M.
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 64, Issue Pt_10
  • DOI: 10.1099/ijs.0.063727-0

Bradyrhizobium iriomotense sp. nov., Isolated from a Tumor-Like Root of the Legume Entada koshunensis from Iriomote Island in Japan
journal, June 2008

  • Islam, Muhammad Saiful; Kawasaki, Hiroko; Muramatsu, Yuki
  • Bioscience, Biotechnology, and Biochemistry, Vol. 72, Issue 6
  • DOI: 10.1271/bbb.70739

MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods
journal, May 2011

  • Tamura, K.; Peterson, D.; Peterson, N.
  • Molecular Biology and Evolution, Vol. 28, Issue 10
  • DOI: 10.1093/molbev/msr121

The minimum information about a genome sequence (MIGS) specification
journal, May 2008

  • Field, Dawn; Garrity, George; Gray, Tanya
  • Nature Biotechnology, Vol. 26, Issue 5
  • DOI: 10.1038/nbt1360

The Genomic Standards Consortium
journal, June 2011


Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.
journal, June 1990

  • Woese, C. R.; Kandler, O.; Wheelis, M. L.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 12
  • DOI: 10.1073/pnas.87.12.4576

Class I. Alphaproteobacteria class. nov.
book, January 2005

  • Garrity, George M.; Bell, Julia A.; Lilburn, Timothy
  • Bergey’s Manual® of Systematic Bacteriology
  • DOI: 10.1007/0-387-29298-5_1

Validation of publication of new names and new combinations previously effectively published outside the IJSEM
journal, November 2005

  • Euzeby, Jean
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 55, Issue 6, p. 2235-2238
  • DOI: 10.1099/ijs.0.64108-0