Functionally conserved enhancers with divergent sequences in distant vertebrates
Abstract
© 2015 Yang et al. Background: To examine the contributions of sequence and function conservation in the evolution of enhancers, we systematically identified enhancers whose sequences are not conserved among distant groups of vertebrate species, but have homologous function and are likely to be derived from a common ancestral sequence. Our approach combined comparative genomics and epigenomics to identify potential enhancer sequences in the genomes of three groups of distantly related vertebrate species. Results: We searched for sequences that were conserved within groups of closely related species but not between groups of more distant species, and were associated with an epigenetic mark of enhancer activity. To facilitate inferring orthology between non-conserved sequences, we limited our search to introns whose orthology could be unambiguously established by mapping the bracketing exons. We show that a subset of these non-conserved but syntenic sequences from the mouse and zebrafish genomes have homologous functions in a zebrafish transgenic enhancer assay. The conserved expression patterns driven by these enhancers are probably associated with short transcription factor-binding motifs present in the divergent sequences. Conclusions: We have identified numerous potential enhancers with divergent sequences but a conserved function. These results indicate that selection on function, rather than sequence,more »
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1618538
- Alternate Identifier(s):
- OSTI ID: 1256483; OSTI ID: 1378614
- Grant/Contract Number:
- AC02-05CH11231; HL091495; R01HG005058; 1R01HG006768; R01HD059862; GM61390; 1R01DK090382; 1R01NS079231
- Resource Type:
- Published Article
- Journal Name:
- BMC Genomics
- Additional Journal Information:
- Journal Name: BMC Genomics Journal Volume: 16 Journal Issue: 1; Journal ID: ISSN 1471-2164
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Yang, Song, Oksenberg, Nir, Takayama, Sachiko, Heo, Seok-Jin, Poliakov, Alexander, Ahituv, Nadav, Dubchak, Inna, and Boffelli, Dario. Functionally conserved enhancers with divergent sequences in distant vertebrates. United Kingdom: N. p., 2015.
Web. doi:10.1186/s12864-015-2070-7.
Yang, Song, Oksenberg, Nir, Takayama, Sachiko, Heo, Seok-Jin, Poliakov, Alexander, Ahituv, Nadav, Dubchak, Inna, & Boffelli, Dario. Functionally conserved enhancers with divergent sequences in distant vertebrates. United Kingdom. https://doi.org/10.1186/s12864-015-2070-7
Yang, Song, Oksenberg, Nir, Takayama, Sachiko, Heo, Seok-Jin, Poliakov, Alexander, Ahituv, Nadav, Dubchak, Inna, and Boffelli, Dario. Fri .
"Functionally conserved enhancers with divergent sequences in distant vertebrates". United Kingdom. https://doi.org/10.1186/s12864-015-2070-7.
@article{osti_1618538,
title = {Functionally conserved enhancers with divergent sequences in distant vertebrates},
author = {Yang, Song and Oksenberg, Nir and Takayama, Sachiko and Heo, Seok-Jin and Poliakov, Alexander and Ahituv, Nadav and Dubchak, Inna and Boffelli, Dario},
abstractNote = {© 2015 Yang et al. Background: To examine the contributions of sequence and function conservation in the evolution of enhancers, we systematically identified enhancers whose sequences are not conserved among distant groups of vertebrate species, but have homologous function and are likely to be derived from a common ancestral sequence. Our approach combined comparative genomics and epigenomics to identify potential enhancer sequences in the genomes of three groups of distantly related vertebrate species. Results: We searched for sequences that were conserved within groups of closely related species but not between groups of more distant species, and were associated with an epigenetic mark of enhancer activity. To facilitate inferring orthology between non-conserved sequences, we limited our search to introns whose orthology could be unambiguously established by mapping the bracketing exons. We show that a subset of these non-conserved but syntenic sequences from the mouse and zebrafish genomes have homologous functions in a zebrafish transgenic enhancer assay. The conserved expression patterns driven by these enhancers are probably associated with short transcription factor-binding motifs present in the divergent sequences. Conclusions: We have identified numerous potential enhancers with divergent sequences but a conserved function. These results indicate that selection on function, rather than sequence, may be a common mode of enhancer evolution; evidence for selection at the sequence level is not a necessary criterion to define a gene regulatory element.},
doi = {10.1186/s12864-015-2070-7},
journal = {BMC Genomics},
number = 1,
volume = 16,
place = {United Kingdom},
year = {Fri Oct 30 00:00:00 EDT 2015},
month = {Fri Oct 30 00:00:00 EDT 2015}
}
https://doi.org/10.1186/s12864-015-2070-7
Web of Science
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