In vivo observation of tree drought response with low-field NMR and neutron imaging
Abstract
Using a simple low-field NMR system, we monitored water content in a living tree in a greenhouse over 2 months. By continuously running the system, we observed changes in tree water content on a scale of half an hour. The data showed a diurnal change in water content consistent both with previous NMR and biological observations. Neutron imaging experiments show that our NMR signal is primarily due to water being rapidly transported through the plant, and not to other sources of hydrogen, such as water in cytoplasm, or water in cell walls. After accounting for the role of temperature in the observed NMR signal, we demonstrate a change in the diurnal signal behavior due to simulated drought conditions for the tree. Lastly, these results illustrate the utility of our system to perform noninvasive measurements of tree water content outside of a temperature controlled environment.
- Authors:
-
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1256106
- Report Number(s):
- LA-UR-16-20572
Journal ID: ISSN 1664-462X
- Grant/Contract Number:
- AC52-06NA25396
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Biological Science
Citation Formats
Malone, Michael W., Yoder, Jacob, Hunter, James F., Espy, Michelle A., Dickman, Lee T., Nelson, Ron O., Vogel, Sven C., Sandin, Henrik J., and Sevanto, Sanna. In vivo observation of tree drought response with low-field NMR and neutron imaging. United States: N. p., 2016.
Web. doi:10.3389/fpls.2016.00564.
Malone, Michael W., Yoder, Jacob, Hunter, James F., Espy, Michelle A., Dickman, Lee T., Nelson, Ron O., Vogel, Sven C., Sandin, Henrik J., & Sevanto, Sanna. In vivo observation of tree drought response with low-field NMR and neutron imaging. United States. https://doi.org/10.3389/fpls.2016.00564
Malone, Michael W., Yoder, Jacob, Hunter, James F., Espy, Michelle A., Dickman, Lee T., Nelson, Ron O., Vogel, Sven C., Sandin, Henrik J., and Sevanto, Sanna. Fri .
"In vivo observation of tree drought response with low-field NMR and neutron imaging". United States. https://doi.org/10.3389/fpls.2016.00564. https://www.osti.gov/servlets/purl/1256106.
@article{osti_1256106,
title = {In vivo observation of tree drought response with low-field NMR and neutron imaging},
author = {Malone, Michael W. and Yoder, Jacob and Hunter, James F. and Espy, Michelle A. and Dickman, Lee T. and Nelson, Ron O. and Vogel, Sven C. and Sandin, Henrik J. and Sevanto, Sanna},
abstractNote = {Using a simple low-field NMR system, we monitored water content in a living tree in a greenhouse over 2 months. By continuously running the system, we observed changes in tree water content on a scale of half an hour. The data showed a diurnal change in water content consistent both with previous NMR and biological observations. Neutron imaging experiments show that our NMR signal is primarily due to water being rapidly transported through the plant, and not to other sources of hydrogen, such as water in cytoplasm, or water in cell walls. After accounting for the role of temperature in the observed NMR signal, we demonstrate a change in the diurnal signal behavior due to simulated drought conditions for the tree. Lastly, these results illustrate the utility of our system to perform noninvasive measurements of tree water content outside of a temperature controlled environment.},
doi = {10.3389/fpls.2016.00564},
journal = {Frontiers in Plant Science},
number = ,
volume = 7,
place = {United States},
year = {Fri May 06 00:00:00 EDT 2016},
month = {Fri May 06 00:00:00 EDT 2016}
}
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In situ plant water balance studies using a portable NMR spectrometer
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Cavitation induced by a surfactant leads to a transient release of water stress and subsequent ‘run away’ embolism in Scots pine (Pinus sylvestris) seedlings
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Cavitation: a blessing in disguise? New method to establish vulnerability curves and assess hydraulic capacitance of woody tissues
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Using Portable nmr to Measure sap flow and Water in the Intact Plant
journal, May 2013
- Windt, C. W.; Blümler, P.
- Acta Horticulturae, Issue 991
Relationships between Embolism, Stem Water Tension, and Diameter Changes
journal, March 2002
- HÖLttÄ, T.; Vesala, T.; PerÄMÄKi, M.
- Journal of Theoretical Biology, Vol. 215, Issue 1
Quantitative neutron imaging of water distribution, venation network and sap flow in leaves
journal, June 2014
- Defraeye, Thijs; Derome, Dominique; Aregawi, Wondwosen
- Planta, Vol. 240, Issue 2
A portable Halbach magnet that can be opened and closed without force: The NMR-CUFF
journal, January 2011
- Windt, Carel W.; Soltner, Helmut; Dusschoten, Dagmar van
- Journal of Magnetic Resonance, Vol. 208, Issue 1
A transportable magnetic resonance imaging system for in situ measurements of living trees: The Tree Hugger
journal, May 2012
- Jones, M.; Aptaker, P. S.; Cox, J.
- Journal of Magnetic Resonance, Vol. 218
MRI of plants and foods
journal, April 2013
- Van As, Henk; van Duynhoven, John
- Journal of Magnetic Resonance, Vol. 229
Time lags for xylem and stem diameter variations in a Scots pine tree
journal, August 2002
- Sevanto, S.; Vesala, T.; Peramaki, M.
- Plant, Cell and Environment, Vol. 25, Issue 8
Development of a mobile magnetic resonance imaging system for outdoor tree measurements
journal, May 2011
- Kimura, Takeshi; Geya, Yuto; Terada, Yasuhiko
- Review of Scientific Instruments, Vol. 82, Issue 5
Low-field nuclear magnetic resonance for the in vivo study of water content in trees
journal, September 2014
- Yoder, Jacob; Malone, Michael W.; Espy, Michelle A.
- Review of Scientific Instruments, Vol. 85, Issue 9
In situ plant water balance studies using a portable NMR spectrometer
journal, January 1994
- Van As, H.; Reinders, J. E. A.; de Jager, P. A.
- Journal of Experimental Botany, Vol. 45, Issue 1
Experimental analysis of the role of water and carbon in tree stem diameter variations
journal, November 2004
- Daudet, F. -A.
- Journal of Experimental Botany
Intact plant MRI for the study of cell water relations, membrane permeability, cell-to-cell and long distance water transport
journal, November 2006
- Van As, H.
- Journal of Experimental Botany, Vol. 58, Issue 4
Cavitation induced by a surfactant leads to a transient release of water stress and subsequent ‘run away’ embolism in Scots pine (Pinus sylvestris) seedlings
journal, October 2011
- Hölttä, Teemu; Juurola, Eija; Lindfors, Lauri
- Journal of Experimental Botany, Vol. 63, Issue 2
MRI links stem water content to stem diameter variations in transpiring trees
journal, January 2012
- De Schepper, Veerle; van Dusschoten, Dagmar; Copini, Paul
- Journal of Experimental Botany, Vol. 63, Issue 7
Modeling tree water deficit from microclimate: an approach to quantifying drought stress
journal, February 2005
- Zweifel, R.; Zimmermann, L.; Newbery, D. M.
- Tree Physiology, Vol. 25, Issue 2
Cavitation: a blessing in disguise? New method to establish vulnerability curves and assess hydraulic capacitance of woody tissues
journal, July 2014
- Vergeynst, L. L.; Dierick, M.; Bogaerts, J. A. N.
- Tree Physiology, Vol. 35, Issue 4
Most Water in the Tomato Truss Is Imported through the Xylem, Not the Phloem: A Nuclear Magnetic Resonance Flow Imaging Study
journal, August 2009
- Windt, Carel W.; Gerkema, Edo; Van As, Henk
- Plant Physiology, Vol. 151, Issue 2
The Developmental Process of Xylem Embolisms in Pine Wilt Disease Monitored by Multipoint Imaging Using Compact Magnetic Resonance Imaging
journal, April 2011
- Umebayashi, Toshihiro; Fukuda, Kenji; Haishi, Tomoyuki
- Plant Physiology, Vol. 156, Issue 2
MRI of long-distance water transport: a comparison of the phloem and xylem flow characteristics and dynamics in poplar, castor bean, tomato and tobacco
journal, September 2006
- Windt, Carel W.; Vergeldt, Frank J.; De Jager, P. Adrie
- Plant, Cell and Environment, Vol. 29, Issue 9
Capacitive effect of cavitation in xylem conduits: results from a dynamic model
journal, January 2009
- HÖLttÄ, Teemu; Cochard, Herve; Nikinmaa, Eero
- Plant, Cell & Environment, Vol. 32, Issue 1
The Kinetics of Rehydration of Detached Sunflower Leaves Following Substantial Water loss
journal, February 1980
- Cruiziat, P.; Tyree, M. T.; Bodet, C.
- New Phytologist, Vol. 84, Issue 2
Interactions Among Scolytid bark Beetles, Their Associated Fungi, and live host Conifers
journal, January 1997
- Paine, T. D.; Raffa, K. F.; Harrington, T. C.
- Annual Review of Entomology, Vol. 42, Issue 1
Vulnerability of Xylem to Cavitation and Embolism
journal, June 1989
- Tyree, M. T.; Sperry, J. S.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 40, Issue 1
Physiological effects of deuterium on dogs
journal, August 1961
- Czajka, Dorice M.; Finkel, Asher J.; Fischer, Conrad S.
- American Journal of Physiology-Legacy Content, Vol. 201, Issue 2
Using Portable nmr to Measure sap flow and Water in the Intact Plant
journal, May 2013
- Windt, C. W.; Blümler, P.
- Acta Horticulturae, Issue 991
Works referencing / citing this record:
Drought impacts on tree phloem: from cell-level responses to ecological significance
journal, February 2019
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- Tree Physiology, Vol. 39, Issue 2
Is desiccation tolerance and avoidance reflected in xylem and phloem anatomy of two coexisting arid-zone coniferous trees?: Xylem and Phloem Anatomy
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- Sevanto, Sanna; Ryan, Max; Dickman, L. Turin
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Neutron Imaging at LANSCE—From Cold to Ultrafast
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In-Vivo NMR Spectroscopy: A Powerful and Complimentary Tool for Understanding Environmental Toxicity
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