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Title: Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke

Abstract

Thirdhand smoke (THS) is a new term for the toxins in cigarette smoke that linger in the environment long after the cigarettes are extinguished. The effects of THS exposure on male reproduction have not yet been studied. In this study, metabolic changes in male germ cell lines (GC-2 and TM-4) were analyzed after THS treatment for 24 h. THS-loaded chromatography paper samples were generated in a laboratory chamber system and extracted in DMEM. At a paper: DMEM ratio of 50 μg/ml, cell viability in both cell lines was normal, as measured by the MTT assay and markers of cytotoxicity, cell cycle, apoptosis and ROS production were normal as measured by quantitative immunofluorescence. Metabolomic analysis was performed on methanol extracts of GC-2 and TM-4 cells. Furthermore, glutathione metabolism in GC-2 cells, and nucleic acid and ammonia metabolism in TM-4 cells, was changed significantly by THS treatment. RT-PCR analyses of mRNA for enzyme genes Gss and Ggt in GC-2 cells, and TK, SMS and Glna in TM-4 cells reinforced these findings, showing changes in the levels of enzymes involved in the relevant pathways. In conclusion, exposure to THS at very low concentrations caused distinct metabolic changes in two different types of malemore » reproductive cell lines.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [3];  [3];  [1]
  1. Nanjing Medical Univ., Nanjing (China)
  2. Univ. of California, San Francisco, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1256060
Alternate Identifier(s):
OSTI ID: 1378601
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Xu, Bo, Chen, Minjian, Yao, Mengmeng, Ji, Xiaoli, Mao, Zhilei, Tang, Wei, Qiao, Shanlei, Schick, Suzaynn F., Mao, Jian-Hua, Hang, Bo, and Xia, Yankai. Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke. United States: N. p., 2015. Web. doi:10.1038/srep15512.
Xu, Bo, Chen, Minjian, Yao, Mengmeng, Ji, Xiaoli, Mao, Zhilei, Tang, Wei, Qiao, Shanlei, Schick, Suzaynn F., Mao, Jian-Hua, Hang, Bo, & Xia, Yankai. Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke. United States. doi:10.1038/srep15512.
Xu, Bo, Chen, Minjian, Yao, Mengmeng, Ji, Xiaoli, Mao, Zhilei, Tang, Wei, Qiao, Shanlei, Schick, Suzaynn F., Mao, Jian-Hua, Hang, Bo, and Xia, Yankai. Thu . "Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke". United States. doi:10.1038/srep15512. https://www.osti.gov/servlets/purl/1256060.
@article{osti_1256060,
title = {Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke},
author = {Xu, Bo and Chen, Minjian and Yao, Mengmeng and Ji, Xiaoli and Mao, Zhilei and Tang, Wei and Qiao, Shanlei and Schick, Suzaynn F. and Mao, Jian-Hua and Hang, Bo and Xia, Yankai},
abstractNote = {Thirdhand smoke (THS) is a new term for the toxins in cigarette smoke that linger in the environment long after the cigarettes are extinguished. The effects of THS exposure on male reproduction have not yet been studied. In this study, metabolic changes in male germ cell lines (GC-2 and TM-4) were analyzed after THS treatment for 24 h. THS-loaded chromatography paper samples were generated in a laboratory chamber system and extracted in DMEM. At a paper: DMEM ratio of 50 μg/ml, cell viability in both cell lines was normal, as measured by the MTT assay and markers of cytotoxicity, cell cycle, apoptosis and ROS production were normal as measured by quantitative immunofluorescence. Metabolomic analysis was performed on methanol extracts of GC-2 and TM-4 cells. Furthermore, glutathione metabolism in GC-2 cells, and nucleic acid and ammonia metabolism in TM-4 cells, was changed significantly by THS treatment. RT-PCR analyses of mRNA for enzyme genes Gss and Ggt in GC-2 cells, and TK, SMS and Glna in TM-4 cells reinforced these findings, showing changes in the levels of enzymes involved in the relevant pathways. In conclusion, exposure to THS at very low concentrations caused distinct metabolic changes in two different types of male reproductive cell lines.},
doi = {10.1038/srep15512},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {2015},
month = {10}
}

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Works referenced in this record:

Formation of carcinogens indoors by surface-mediated reactions of nicotine with nitrous acid, leading to potential thirdhand smoke hazards
journal, February 2010

  • Sleiman, M.; Gundel, L. A.; Pankow, J. F.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 15
  • DOI: 10.1073/pnas.0912820107

Lipid peroxidation and antioxidant responses in zebrafish brain induced by Aphanizomenon flos-aquae DC-1 aphantoxins
journal, November 2013


Inhibition of salivary enzymes by cigarette smoke and the protective role of glutathione
journal, January 2002

  • Zappacosta, B.; Persichilli, S.; Mordente, A.
  • Human & Experimental Toxicology, Vol. 21, Issue 1
  • DOI: 10.1191/0960327102ht202oa

Ammonia toxicity in fish
journal, September 2002


Residual tobacco smoke pollution in used cars for sale: Air, dust, and surfaces
journal, September 2008

  • Matt, Georg; Quintana, Penelope; Hovell, Melbourne
  • Nicotine & Tobacco Research, Vol. 10, Issue 9
  • DOI: 10.1080/14622200802279898

Relationship of Human Toenail Nicotine, Cotinine, and 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanol to Levels of These Biomarkers in Plasma and Urine
journal, July 2007


A Combined Proteomics and Metabolomics Profiling of Gastric Cardia Cancer Reveals Characteristic Dysregulations in Glucose Metabolism
journal, August 2010

  • Cai, Zhen; Zhao, Jiang-Sha; Li, Jing-Jing
  • Molecular & Cellular Proteomics, Vol. 9, Issue 12
  • DOI: 10.1074/mcp.M110.000661

Second-hand smoke and human lung cancer
journal, July 2008


Thirdhand Tobacco Smoke: Emerging Evidence and Arguments for a Multidisciplinary Research Agenda
journal, September 2011

  • Matt, Georg E.; Quintana, Penelope J. E.; Destaillats, Hugo
  • Environmental Health Perspectives, Vol. 119, Issue 9
  • DOI: 10.1289/ehp.1103500

Cardiovascular Effects of Secondhand Smoke: Nearly as Large as Smoking
journal, May 2005


Glutathione metabolism and its role in hepatotoxicity
journal, December 1991


Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells
journal, September 2013

  • Tan, Caihong; Qian, Xiaoqiang; Jia, Rongdi
  • Oncology Reports, Vol. 30, Issue 5
  • DOI: 10.3892/or.2013.2727

Thirdhand smoke causes DNA damage in human cells
journal, March 2013


Cigarette Smoke Toxins Deposited on Surfaces: Implications for Human Health
journal, January 2014


Association between parental smoking behavior and children's respiratory morbidity: 5-year study in an urban city of South Korea
journal, October 2011

  • Jung, Jae Woo; Ju, Young Su; Kang, Hye Ryun
  • Pediatric Pulmonology, Vol. 47, Issue 4
  • DOI: 10.1002/ppul.21556

Human urinary carcinogen metabolites: biomarkers for investigating tobacco and cancer
journal, June 2002


Effects of Maternal Smoking during Pregnancy and Environmental Tobacco Smoke on Asthma and Wheezing in Children
journal, February 2001

  • Gilliland, Frank D.; Li, Yu-Fen; Peters, John M.
  • American Journal of Respiratory and Critical Care Medicine, Vol. 163, Issue 2
  • DOI: 10.1164/ajrccm.163.2.2006009

When smokers move out and non-smokers move in: residential thirdhand smoke pollution and exposure
journal, October 2010

  • Matt, G. E.; Quintana, P. J. E.; Zakarian, J. M.
  • Tobacco Control, Vol. 20, Issue 1
  • DOI: 10.1136/tc.2010.037382

Disorders of purine and pyrimidine metabolism
journal, September 2005


Doses of Nicotine and Lung Carcinogens Delivered to Cigarette Smokers
journal, January 2000

  • Djordjevic, Mirjana V.; Stellman, Steven D.; Zang, Edith
  • JNCI: Journal of the National Cancer Institute, Vol. 92, Issue 2
  • DOI: 10.1093/jnci/92.2.106

Genotoxicity of tobacco smoke and tobacco smoke condensate: a review
journal, November 2004


Interlaboratory comparability of serum cotinine measurements at smoker and nonsmoker concentration levels: A round-robin study
journal, November 2009

  • Bernert, John T.; Jacob, Peyton; Holiday, David B.
  • Nicotine & Tobacco Research, Vol. 11, Issue 12
  • DOI: 10.1093/ntr/ntp161

Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression
journal, February 2009

  • Sreekumar, Arun; Poisson, Laila M.; Rajendiran, Thekkelnaycke M.
  • Nature, Vol. 457, Issue 7231
  • DOI: 10.1038/nature07762

Oxidative Stress Produced by Marijuana Smoke: An Adverse Effect Enhanced by Cannabinoids
journal, June 1999

  • Sarafian, Theodore A.; Magallanes, Jose Antonio Marques; Shau, Hungyi
  • American Journal of Respiratory Cell and Molecular Biology, Vol. 20, Issue 6
  • DOI: 10.1165/ajrcmb.20.6.3424

Genotoxicity of environmental tobacco smoke: a review
journal, November 2004