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Title: Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry

The ability to image light elements in soft matter at atomic resolution enables unprecedented insight into the structure and properties of molecular heterostructures and beam-sensitive nanomaterials. In this study, we introduce a scanning transmission electron microscopy technique combining a pre-specimen phase plate designed to produce a probe with structured phase with a high-speed direct electron detector to generate nearly linear contrast images with high efficiency. We demonstrate this method by using both experiment and simulation to simultaneously image the atomic-scale structure of weakly scattering amorphous carbon and strongly scattering gold nanoparticles. Our method demonstrates strong contrast for both materials, making it a promising candidate for structural determination of heterogeneous soft/hard matter samples even at low electron doses comparable to traditional phase-contrast transmission electron microscopy. Ultimately, simulated images demonstrate the extension of this technique to the challenging problem of structural determination of biological material at the surface of inorganic crystals.
Authors:
 [1] ;  [1] ;  [2] ;  [2] ;  [2] ;  [2] ;  [3] ;  [4] ;  [1]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  2. Univ. of Oregon, Eugene, OR (United States)
  3. Gatan Inc., Pleasanton, CA (United States)
  4. Ulm Univ., Ulm (Germany)
Publication Date:
Grant/Contract Number:
AC02-05CH11231; SC0010466
Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY
OSTI Identifier:
1255550
Alternate Identifier(s):
OSTI ID: 1379111

Ophus, Colin, Ciston, Jim, Pierce, Jordan, Harvey, Tyler R., Chess, Jordan, McMorran, Benjamin J., Czarnik, Cory, Rose, Harald H., and Ercius, Peter. Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry. United States: N. p., Web. doi:10.1038/ncomms10719.
Ophus, Colin, Ciston, Jim, Pierce, Jordan, Harvey, Tyler R., Chess, Jordan, McMorran, Benjamin J., Czarnik, Cory, Rose, Harald H., & Ercius, Peter. Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry. United States. doi:10.1038/ncomms10719.
Ophus, Colin, Ciston, Jim, Pierce, Jordan, Harvey, Tyler R., Chess, Jordan, McMorran, Benjamin J., Czarnik, Cory, Rose, Harald H., and Ercius, Peter. 2016. "Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry". United States. doi:10.1038/ncomms10719. https://www.osti.gov/servlets/purl/1255550.
@article{osti_1255550,
title = {Efficient linear phase contrast in scanning transmission electron microscopy with matched illumination and detector interferometry},
author = {Ophus, Colin and Ciston, Jim and Pierce, Jordan and Harvey, Tyler R. and Chess, Jordan and McMorran, Benjamin J. and Czarnik, Cory and Rose, Harald H. and Ercius, Peter},
abstractNote = {The ability to image light elements in soft matter at atomic resolution enables unprecedented insight into the structure and properties of molecular heterostructures and beam-sensitive nanomaterials. In this study, we introduce a scanning transmission electron microscopy technique combining a pre-specimen phase plate designed to produce a probe with structured phase with a high-speed direct electron detector to generate nearly linear contrast images with high efficiency. We demonstrate this method by using both experiment and simulation to simultaneously image the atomic-scale structure of weakly scattering amorphous carbon and strongly scattering gold nanoparticles. Our method demonstrates strong contrast for both materials, making it a promising candidate for structural determination of heterogeneous soft/hard matter samples even at low electron doses comparable to traditional phase-contrast transmission electron microscopy. Ultimately, simulated images demonstrate the extension of this technique to the challenging problem of structural determination of biological material at the surface of inorganic crystals.},
doi = {10.1038/ncomms10719},
journal = {Nature Communications},
number = ,
volume = 7,
place = {United States},
year = {2016},
month = {2}
}

Works referenced in this record:

Efficient phase contrast imaging in STEM using a pixelated detector. Part 1: Experimental demonstration at atomic resolution
journal, April 2015

Electron Vortex Beams with High Quanta of Orbital Angular Momentum
journal, January 2011
  • McMorran, B. J.; Agrawal, A.; Anderson, I. M.
  • Science, Vol. 331, Issue 6014, p. 192-195
  • DOI: 10.1126/science.1198804

Direct least square fitting of ellipses
journal, May 1999
  • Fitzgibbon, A.; Pilu, M.; Fisher, R. B.
  • IEEE Transactions on Pattern Analysis and Machine Intelligence, Vol. 21, Issue 5, p. 476-480
  • DOI: 10.1109/34.765658