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Title: Genome sequence of Bradyrhizobium sp. WSM1253; a microsymbiont of Ornithopus compressus from the Greek Island of Sifnos

Abstract

Bradyrhizobium sp. WSM1253 is a novel N 2-fixing bacterium isolated from a root nodule of the herbaceous annual legume Ornithopus compressus that was growing on the Greek Island of Sifnos. WSM1253 emerged as a strain of interest in an Australian program that was selecting inoculant quality bradyrhizobial strains for inoculation of Mediterranean species of lupins ( Lupinus angustifolius, L. princei, L. atlanticus, L. pilosus ). In this report we describe, for the first time, the genome sequence information and annotation of this legume microsymbiont. The 8,719,808 bp genome has a G + C content of 63.09 % with 71 contigs arranged into two scaffolds. The assembled genome contains 8,432 protein-coding genes, 66 RNA genes and a single rRNA operon. In conclusion, this improved-high-quality draft rhizobial genome is one of 20 sequenced through a DOE Joint Genome Institute 2010 Community Sequencing Project.

Authors:
 [1];  [1];  [2];  [1];  [3];  [3];  [3];  [4];  [4];  [4];  [4];  [4];  [5];  [4];  [6];  [1]
  1. Murdoch Univ., Murdoch (Australia). Centre for Rhizobium Studies
  2. Murdoch Univ., Murdoch (Australia). Centre for Rhizobium Studies; Department of Agriculture and Food, South Perth (Australia)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  4. DOE Joint Genome Institute, Walnut Creek, CA (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  6. DOE Joint Genome Institute, Walnut Creek, CA (United States); King Abdulaziz University, Jeddah (Saudi Arabia)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1255537
Alternate Identifier(s):
OSTI ID: 1378661
Grant/Contract Number:  
AC02-05CH11231; AC52-07NA27344; AC02-06NA25396
Resource Type:
Accepted Manuscript
Journal Name:
Standards in Genomic Sciences
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1944-3277
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; root-nodule bacteria; nitrogen fixation; rhizobia; Ornithopus

Citation Formats

Tiwari, Ravi, Howieson, John, Yates, Ron, Tian, Rui, Held, Britanny, Tapia, Roxanne, Han, Cliff, Seshadri, Rekha, Reddy, T. B. K., Huntemann, Marcel, Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. Genome sequence of Bradyrhizobium sp. WSM1253; a microsymbiont of Ornithopus compressus from the Greek Island of Sifnos. United States: N. p., 2015. Web. doi:10.1186/s40793-015-0115-9.
Tiwari, Ravi, Howieson, John, Yates, Ron, Tian, Rui, Held, Britanny, Tapia, Roxanne, Han, Cliff, Seshadri, Rekha, Reddy, T. B. K., Huntemann, Marcel, Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, & Reeve, Wayne. Genome sequence of Bradyrhizobium sp. WSM1253; a microsymbiont of Ornithopus compressus from the Greek Island of Sifnos. United States. doi:10.1186/s40793-015-0115-9.
Tiwari, Ravi, Howieson, John, Yates, Ron, Tian, Rui, Held, Britanny, Tapia, Roxanne, Han, Cliff, Seshadri, Rekha, Reddy, T. B. K., Huntemann, Marcel, Pati, Amrita, Woyke, Tanja, Markowitz, Victor, Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. Mon . "Genome sequence of Bradyrhizobium sp. WSM1253; a microsymbiont of Ornithopus compressus from the Greek Island of Sifnos". United States. doi:10.1186/s40793-015-0115-9. https://www.osti.gov/servlets/purl/1255537.
@article{osti_1255537,
title = {Genome sequence of Bradyrhizobium sp. WSM1253; a microsymbiont of Ornithopus compressus from the Greek Island of Sifnos},
author = {Tiwari, Ravi and Howieson, John and Yates, Ron and Tian, Rui and Held, Britanny and Tapia, Roxanne and Han, Cliff and Seshadri, Rekha and Reddy, T. B. K. and Huntemann, Marcel and Pati, Amrita and Woyke, Tanja and Markowitz, Victor and Ivanova, Natalia and Kyrpides, Nikos and Reeve, Wayne},
abstractNote = {Bradyrhizobium sp. WSM1253 is a novel N2-fixing bacterium isolated from a root nodule of the herbaceous annual legume Ornithopus compressus that was growing on the Greek Island of Sifnos. WSM1253 emerged as a strain of interest in an Australian program that was selecting inoculant quality bradyrhizobial strains for inoculation of Mediterranean species of lupins ( Lupinus angustifolius, L. princei, L. atlanticus, L. pilosus ). In this report we describe, for the first time, the genome sequence information and annotation of this legume microsymbiont. The 8,719,808 bp genome has a G + C content of 63.09 % with 71 contigs arranged into two scaffolds. The assembled genome contains 8,432 protein-coding genes, 66 RNA genes and a single rRNA operon. In conclusion, this improved-high-quality draft rhizobial genome is one of 20 sequenced through a DOE Joint Genome Institute 2010 Community Sequencing Project.},
doi = {10.1186/s40793-015-0115-9},
journal = {Standards in Genomic Sciences},
number = 1,
volume = 10,
place = {United States},
year = {2015},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Figures / Tables:

Fig. 1 Fig. 1: Images of Bradyrhizobium sp. WSM1253 using scanning (Left) and transmission (Center) electron microscopy as well as light microscopy to visualize colony morphology on solid media (Right)

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    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.