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Title: High-Quality draft genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain CJ3Sym

Abstract

Mesorhizobium loti strain CJ3Sym was isolated in 1998 following transfer of the integrative and conjugative element ICE Ml Sym R7A , also known as the R7A symbiosis island, in a laboratory mating from the donor M. loti strain R7A to a nonsymbiotic recipient Mesorhizobium strain CJ3. Strain CJ3 was originally isolated from a field site in the Rocklands range in New Zealand in 1994. CJ3Sym is an aerobic, Gram-negative, non-spore-forming rod. This report reveals the genome of M. loti strain CJ3Sym currently comprises 70 scaffolds totaling 7,563,725 bp. In conclusion, the high-quality draft genome is arranged in 70 scaffolds of 71 contigs, contains 7,331 protein-coding genes and 70 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.

Authors:
 [1];  [2];  [2];  [1];  [3];  [3];  [4];  [4];  [4];  [4];  [5];  [4];  [6]
  1. Murdoch Univ. Perth, WA (Australia). Centre for Rhizobium Studies
  2. Univ. of Otago, Dunedin (New Zealand)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  4. DOE Joint Genome Institute, Walnut Creek, CA (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  6. DOE Joint Genome Institute, Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1255535
Grant/Contract Number:  
AC02-05CH11231; AC52-07NA27344; AC02-06NA25396
Resource Type:
Accepted Manuscript
Journal Name:
Standards in Genomic Sciences
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1944-3277
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Root-nodule bacteria; Nitrogen fixation; Symbiosis; Alphaproteobacteria; GEBA-RNB

Citation Formats

Reeve, Wayne, Sullivan, John, Ronson, Clive, Tian, Rui, Munk, Christine, Han, Cliff, Reddy, T. B. K., Seshadri, Rekha, Woyke, Tanja, Pati, Amrita, Markowitz, Victor, Ivanova, Natalia, and Kyrpides, Nikos. High-Quality draft genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain CJ3Sym. United States: N. p., 2015. Web. doi:10.1186/s40793-015-0049-2.
Reeve, Wayne, Sullivan, John, Ronson, Clive, Tian, Rui, Munk, Christine, Han, Cliff, Reddy, T. B. K., Seshadri, Rekha, Woyke, Tanja, Pati, Amrita, Markowitz, Victor, Ivanova, Natalia, & Kyrpides, Nikos. High-Quality draft genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain CJ3Sym. United States. doi:10.1186/s40793-015-0049-2.
Reeve, Wayne, Sullivan, John, Ronson, Clive, Tian, Rui, Munk, Christine, Han, Cliff, Reddy, T. B. K., Seshadri, Rekha, Woyke, Tanja, Pati, Amrita, Markowitz, Victor, Ivanova, Natalia, and Kyrpides, Nikos. Fri . "High-Quality draft genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain CJ3Sym". United States. doi:10.1186/s40793-015-0049-2. https://www.osti.gov/servlets/purl/1255535.
@article{osti_1255535,
title = {High-Quality draft genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain CJ3Sym},
author = {Reeve, Wayne and Sullivan, John and Ronson, Clive and Tian, Rui and Munk, Christine and Han, Cliff and Reddy, T. B. K. and Seshadri, Rekha and Woyke, Tanja and Pati, Amrita and Markowitz, Victor and Ivanova, Natalia and Kyrpides, Nikos},
abstractNote = {Mesorhizobium loti strain CJ3Sym was isolated in 1998 following transfer of the integrative and conjugative element ICE Ml SymR7A , also known as the R7A symbiosis island, in a laboratory mating from the donor M. loti strain R7A to a nonsymbiotic recipient Mesorhizobium strain CJ3. Strain CJ3 was originally isolated from a field site in the Rocklands range in New Zealand in 1994. CJ3Sym is an aerobic, Gram-negative, non-spore-forming rod. This report reveals the genome of M. loti strain CJ3Sym currently comprises 70 scaffolds totaling 7,563,725 bp. In conclusion, the high-quality draft genome is arranged in 70 scaffolds of 71 contigs, contains 7,331 protein-coding genes and 70 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.},
doi = {10.1186/s40793-015-0049-2},
journal = {Standards in Genomic Sciences},
number = 1,
volume = 10,
place = {United States},
year = {2015},
month = {8}
}

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Works referenced in this record:

Confidence Limits on Phylogenies: An Approach Using the Bootstrap
journal, July 1985

  • Felsenstein, Joseph
  • Evolution, Vol. 39, Issue 4, p. 783-791
  • DOI: 10.2307/2408678