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Title: Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments

To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. In conclusion, we also demonstrate that cysteine-functionalized C60- pyrrolidine tris acid can differentiate live and dead microorganisms.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [1]
  1. Univ. of Nevada, Reno, NV (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Brookhaven National Lab. (BNL), Upton, NY (United States)
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1255532

Cheng, Qingsu, Aravind, Ashwin, Buckley, Matthew, Gifford, Andrew, and Parvin, Bahram. Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments. United States: N. p., Web. doi:10.1038/srep13685.
Cheng, Qingsu, Aravind, Ashwin, Buckley, Matthew, Gifford, Andrew, & Parvin, Bahram. Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments. United States. doi:10.1038/srep13685.
Cheng, Qingsu, Aravind, Ashwin, Buckley, Matthew, Gifford, Andrew, and Parvin, Bahram. 2015. "Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments". United States. doi:10.1038/srep13685. https://www.osti.gov/servlets/purl/1255532.
@article{osti_1255532,
title = {Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments},
author = {Cheng, Qingsu and Aravind, Ashwin and Buckley, Matthew and Gifford, Andrew and Parvin, Bahram},
abstractNote = {To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. In conclusion, we also demonstrate that cysteine-functionalized C60- pyrrolidine tris acid can differentiate live and dead microorganisms.},
doi = {10.1038/srep13685},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {2015},
month = {9}
}