Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging

Si, Tong; Li, Bin; Zhang, Ke; Xu, Yiran; Zhao, Huimin; Sweedler, Jonathan V.

doi:10.1021/acs.jproteome.6b00127

Title: Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging

Authors:

Si, Tong ^[1]; Li, Bin ^[2]; Zhang, Ke ^[3]; Xu, Yiran ^[4]; Zhao, Huimin ^[5]; Sweedler, Jonathan V. ^[6]

Department of Chemical and Biomolecular Engineering and Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
Department of Chemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
Department of Chemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
Department of Biochemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
Department of Chemical and Biomolecular Engineering and Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States, Department of Chemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States, Department of Biochemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States, Department of Bioengineering, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States
Department of Chemistry, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States, Department of Bioengineering, University of Illinois at Urbana−Champaign, Urbana, Illinois 61801, United States

Publication Date:: Wed May 11 00:00:00 EDT 2016

Sponsoring Org.:: USDOE

OSTI Identifier:: 1254201

Grant/Contract Number:: ORNL-4000134575

Resource Type:: Published Article

Journal Name:: Journal of Proteome Research

Additional Journal Information:: Journal Name: Journal of Proteome Research Journal Volume: 15 Journal Issue: 6; Journal ID: ISSN 1535-3893

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Citation Formats


                    Si, Tong, Li, Bin, Zhang, Ke, Xu, Yiran, Zhao, Huimin, and Sweedler, Jonathan V. Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging.  United States: N. p., 2016. 
Web.  doi:10.1021/acs.jproteome.6b00127.

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                    Si, Tong, Li, Bin, Zhang, Ke, Xu, Yiran, Zhao, Huimin, & Sweedler, Jonathan V. Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging.  United States.  https://doi.org/10.1021/acs.jproteome.6b00127

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                    Si, Tong, Li, Bin, Zhang, Ke, Xu, Yiran, Zhao, Huimin, and Sweedler, Jonathan V. Wed .  
"Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging".  United States.  https://doi.org/10.1021/acs.jproteome.6b00127.

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@article{osti_1254201,

  title        = {Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging},

  author       = {Si, Tong and Li, Bin and Zhang, Ke and Xu, Yiran and Zhao, Huimin and Sweedler, Jonathan V.},

  abstractNote = {},

  doi          = {10.1021/acs.jproteome.6b00127},

  journal      = {Journal of Proteome Research},

  number       = 6,

  volume       = 15,

  place        = {United States},

  year         = {Wed May 11 00:00:00 EDT 2016},

  month        = {Wed May 11 00:00:00 EDT 2016}

}

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Title: Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging

Citation Formats

Control of cell fate by the formation of an architecturally complex bacterial community journal, April 2008

Imaging mass spectrometry in microbiology journal, August 2011

Imaging mass spectrometry of intraspecies metabolic exchange revealed the cannibalistic factors of Bacillus subtilis journal, August 2010

The biofilm matrix journal, August 2010

Sociomicrobiology: the connections between quorum sensing and biofilms journal, January 2005

A major protein component of the Bacillus subtilis biofilm matrix journal, February 2006

A high-throughput gene expression analysis technique using competitive PCR and matrix-assisted laser desorption ionization time-of-flight MS journal, March 2003

Paracrine signaling in a bacterium journal, July 2009

Bacterial biofilms: from the Natural environment to infectious diseases journal, February 2004

Biofilms: the matrix revisited journal, January 2005

Correlated Imaging with C 60 -SIMS and Confocal Raman Microscopy: Visualization of Cell-Scale Molecular Distributions in Bacterial Biofilms journal, October 2014

Lipopeptides as main ingredients for inhibition of fungal phytopathogens by B acillus subtilis/amyloliquefaciens: Lipopeptides as inhibitors of phytopathogens journal, December 2014

Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis journal, October 2008

Biofilms: implications in bioremediation journal, September 2006

Molecular imaging of proteins in tissues by mass spectrometry journal, September 2008

Correlated imaging – a grand challenge in chemical analysis journal, January 2013

The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene. journal, March 1990

Biofilm formation by B acillus subtilis : new insights into regulatory strategies and assembly mechanisms: Regulation and assembly of Bacillus subtilis biofilms journal, July 2014

The developmental model of microbial biofilms: ten years of a paradigm up for review journal, February 2009

Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation journal, September 2010

Spatial organization of Pseudomonas aeruginosa biofilms probed by combined matrix-assisted laser desorption ionization mass spectrometry and confocal Raman microscopy journal, January 2014

Amyloid fibers provide structural integrity to Bacillus subtilis biofilms journal, January 2010

Spatiotemporal Monitoring of the Antibiome Secreted by Bacillus Biofilms on Plant Roots Using MALDI Mass Spectrometry Imaging journal, April 2014

Translating metabolic exchange with imaging mass spectrometry journal, November 2009

Physiological heterogeneity in biofilms journal, March 2008

Imaging mass spectrometry journal, January 2007

Multimodal chemical imaging of molecular messengers in emerging Pseudomonas aeruginosa bacterial communities journal, January 2015

A field guide to bacterial swarming motility journal, August 2010

The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis: Biofilm development in Bacillus subtilis journal, December 2001

Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis: AbrB regulated genes involved in biofilm formation journal, March 2004

Sticking together: building a biofilm the Bacillus subtilis way journal, January 2013

MALDI-guided SIMS: Multiscale Imaging of Metabolites in Bacterial Biofilms journal, August 2014

The dynamic microbe: green fluorescent protein brings bacteria to light: Monitoring microbial dynamics with GFP journal, September 2002

Structurally diverse natural products that cause potassium leakage trigger multicellularity in Bacillus subtilis journal, December 2008

Exopolymeric substances (EPS) from Bacillus subtilis : polymers and genes encoding their synthesis: EPS from Bacillus subtilis journal, November 2010

In situ localisation and quantification of surfactins in a Bacillus subtilis swarming community by imaging mass spectrometry journal, August 2008

Structure of the Bacillus subtilis Peptide Antibiotic Subtilosin A Determined by 1H-NMR and Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry journal, August 2001

Cannibalism enhances biofilm development in Bacillus subtilis : Cannibalism and biofilm matrix journal, September 2009

Biofilms as Complex Differentiated Communities journal, October 2002

Evolving concepts in biofilm infections journal, July 2009

The Spo0A regulon of Bacillus subtilis: The Spo0A regulon journal, November 2003

Fruiting body formation by Bacillus subtilis journal, September 2001

Primer on Agar-Based Microbial Imaging Mass Spectrometry journal, July 2012

Control of cell fate by the formation of an architecturally complex bacterial community
journal, April 2008

Imaging mass spectrometry in microbiology
journal, August 2011

Imaging mass spectrometry of intraspecies metabolic exchange revealed the cannibalistic factors of Bacillus subtilis
journal, August 2010

The biofilm matrix
journal, August 2010

Sociomicrobiology: the connections between quorum sensing and biofilms
journal, January 2005

A major protein component of the Bacillus subtilis biofilm matrix
journal, February 2006

A high-throughput gene expression analysis technique using competitive PCR and matrix-assisted laser desorption ionization time-of-flight MS
journal, March 2003

Paracrine signaling in a bacterium
journal, July 2009

Bacterial biofilms: from the Natural environment to infectious diseases
journal, February 2004

Biofilms: the matrix revisited
journal, January 2005

Correlated Imaging with C ₆₀ -SIMS and Confocal Raman Microscopy: Visualization of Cell-Scale Molecular Distributions in Bacterial Biofilms
journal, October 2014

Lipopeptides as main ingredients for inhibition of fungal phytopathogens by B acillus subtilis/amyloliquefaciens: Lipopeptides as inhibitors of phytopathogens
journal, December 2014

Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis
journal, October 2008

Biofilms: implications in bioremediation
journal, September 2006

Molecular imaging of proteins in tissues by mass spectrometry
journal, September 2008

Correlated imaging – a grand challenge in chemical analysis
journal, January 2013

The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene.
journal, March 1990

Biofilm formation by B acillus subtilis : new insights into regulatory strategies and assembly mechanisms: Regulation and assembly of Bacillus subtilis biofilms
journal, July 2014

The developmental model of microbial biofilms: ten years of a paradigm up for review
journal, February 2009

Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation
journal, September 2010

Spatial organization of Pseudomonas aeruginosa biofilms probed by combined matrix-assisted laser desorption ionization mass spectrometry and confocal Raman microscopy
journal, January 2014

Amyloid fibers provide structural integrity to Bacillus subtilis biofilms
journal, January 2010

Spatiotemporal Monitoring of the Antibiome Secreted by Bacillus Biofilms on Plant Roots Using MALDI Mass Spectrometry Imaging
journal, April 2014

Translating metabolic exchange with imaging mass spectrometry
journal, November 2009

Physiological heterogeneity in biofilms
journal, March 2008

Imaging mass spectrometry
journal, January 2007

Multimodal chemical imaging of molecular messengers in emerging Pseudomonas aeruginosa bacterial communities
journal, January 2015

A field guide to bacterial swarming motility
journal, August 2010

The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis: Biofilm development in Bacillus subtilis
journal, December 2001

Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis: AbrB regulated genes involved in biofilm formation
journal, March 2004

Sticking together: building a biofilm the Bacillus subtilis way
journal, January 2013

MALDI-guided SIMS: Multiscale Imaging of Metabolites in Bacterial Biofilms
journal, August 2014

The dynamic microbe: green fluorescent protein brings bacteria to light: Monitoring microbial dynamics with GFP
journal, September 2002

Structurally diverse natural products that cause potassium leakage trigger multicellularity in Bacillus subtilis
journal, December 2008

Exopolymeric substances (EPS) from Bacillus subtilis : polymers and genes encoding their synthesis: EPS from Bacillus subtilis
journal, November 2010

In situ localisation and quantification of surfactins in a Bacillus subtilis swarming community by imaging mass spectrometry
journal, August 2008

Structure of the Bacillus subtilis Peptide Antibiotic Subtilosin A Determined by 1H-NMR and Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
journal, August 2001

Cannibalism enhances biofilm development in Bacillus subtilis : Cannibalism and biofilm matrix
journal, September 2009

Biofilms as Complex Differentiated Communities
journal, October 2002

Evolving concepts in biofilm infections
journal, July 2009

The Spo0A regulon of Bacillus subtilis: The Spo0A regulon
journal, November 2003

Fruiting body formation by Bacillus subtilis
journal, September 2001

Primer on Agar-Based Microbial Imaging Mass Spectrometry
journal, July 2012