skip to main content

DOE PAGESDOE PAGES

Title: Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution

Here, we have developed a method for performing light-sheet microscopy with a single high numerical aperture lens by integrating reflective side walls into a microfluidic chip. These 45° side walls generate light-sheet illumination by reflecting a vertical light-sheet into the focal plane of the objective. Light-sheet illumination of cells loaded in the channels increases image quality in diffraction limited imaging via reduction of out-of-focus background light. Single molecule super-resolution is also improved by the decreased background resulting in better localization precision and decreased photo-bleaching, leading to more accepted localizations overall and higher quality images. Moreover, 2D and 3D single molecule super-resolution data can be acquired faster by taking advantage of the increased illumination intensities as compared to wide field, in the focused light-sheet.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [1]
  1. Univ. of New Mexico, Albuquerque, NM (United States)
  2. Univ. of New Mexico, Albuquerque, NM (United States); Purdue Univ., West Lafayette, IN (United States)
  3. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Report Number(s):
SAND2016-4996J
Journal ID: ISSN 2156-7085; 640849
Grant/Contract Number:
AC04-94AL85000
Type:
Published Article
Journal Name:
Biomedical Optics Express
Additional Journal Information:
Journal Volume: 7; Journal Issue: 6; Journal ID: ISSN 2156-7085
Publisher:
Optical Society of America
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE National Nuclear Security Administration (NNSA)
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION
OSTI Identifier:
1253399
Alternate Identifier(s):
OSTI ID: 1259471