Characterization and vaccine potential of outer membrane vesicles produced by Haemophilus parasuis
Abstract
Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structures has led to identification of virulence factors in a number of bacteria and they have been successfully used as subunit vaccines. We have isolated OMV from both virulent and avirulent strains of H. parasuis, have examined their protein content and assessed their ability to induce an immune response in the host. Lastly, vaccination with purified OMV derived from the virulent H. parasuis Nagasaki strain provided protection against challenge with a lethal dose of the bacteria.
- Authors:
-
- National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA (United States)
- Univ. Paris Descartes, Paris (France)
- Publication Date:
- Research Org.:
- Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States); Oak Ridge Associated Universities (ORAU), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1252954
- Grant/Contract Number:
- AC05-06OR23100
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 3; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; swine; outer membrane proteins; vaccines; vesicles; virulence factors; proteases; vaccination and immunization; serum proteins
Citation Formats
McCaig, William D., Loving, Crystal L., Hughes, Holly R., Brockmeier, Susan L., and Charbit, Alain. Characterization and vaccine potential of outer membrane vesicles produced by Haemophilus parasuis. United States: N. p., 2016.
Web. doi:10.1371/journal.pone.0149132.
McCaig, William D., Loving, Crystal L., Hughes, Holly R., Brockmeier, Susan L., & Charbit, Alain. Characterization and vaccine potential of outer membrane vesicles produced by Haemophilus parasuis. United States. https://doi.org/10.1371/journal.pone.0149132
McCaig, William D., Loving, Crystal L., Hughes, Holly R., Brockmeier, Susan L., and Charbit, Alain. Tue .
"Characterization and vaccine potential of outer membrane vesicles produced by Haemophilus parasuis". United States. https://doi.org/10.1371/journal.pone.0149132. https://www.osti.gov/servlets/purl/1252954.
@article{osti_1252954,
title = {Characterization and vaccine potential of outer membrane vesicles produced by Haemophilus parasuis},
author = {McCaig, William D. and Loving, Crystal L. and Hughes, Holly R. and Brockmeier, Susan L. and Charbit, Alain},
abstractNote = {Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structures has led to identification of virulence factors in a number of bacteria and they have been successfully used as subunit vaccines. We have isolated OMV from both virulent and avirulent strains of H. parasuis, have examined their protein content and assessed their ability to induce an immune response in the host. Lastly, vaccination with purified OMV derived from the virulent H. parasuis Nagasaki strain provided protection against challenge with a lethal dose of the bacteria.},
doi = {10.1371/journal.pone.0149132},
journal = {PLoS ONE},
number = 3,
volume = 11,
place = {United States},
year = {Tue Mar 01 00:00:00 EST 2016},
month = {Tue Mar 01 00:00:00 EST 2016}
}
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Works referenced in this record:
Update on the diagnosis of Haemophilus parasuis infection in pigs and novel genotyping methods
journal, November 2007
- Olvera, Alex; Segalés, Joaquim; Aragón, Virginia
- The Veterinary Journal, Vol. 174, Issue 3
Haemophilus parasuis exhibits IgA protease activity but lacks homologs of the IgA protease genes of Haemophilus influenzae
journal, December 2011
- Mullins, Michael A.; Register, Karen B.; Bayles, Darrell O.
- Veterinary Microbiology, Vol. 153, Issue 3-4
Immune modulation by bacterial outer membrane vesicles
journal, May 2015
- Kaparakis-Liaskos, Maria; Ferrero, Richard L.
- Nature Reviews Immunology, Vol. 15, Issue 6
Characterization and Comparative Analysis of the Genes Encoding Haemophilus parasuis Outer Membrane Proteins P2 and P5
journal, July 2009
- Mullins, M. A.; Register, K. B.; Bayles, D. O.
- Journal of Bacteriology, Vol. 191, Issue 19
Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heat-stable antigen extracts.
journal, January 1992
- Kielstein, P.; Rapp-Gabrielson, V. J.
- Journal of Clinical Microbiology, Vol. 30, Issue 4
A comprehensive proteome map of the Haemophilus parasuis serovar 5
journal, May 2009
- Zhou, Mingguang; Zhang, Anding; Guo, Yi
- PROTEOMICS, Vol. 9, Issue 10
Shewanella oneidensis MR-1 nanowires are outer membrane and periplasmic extensions of the extracellular electron transport components
journal, August 2014
- Pirbadian, S.; Barchinger, S. E.; Leung, K. M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 35
Intranasal Immunization with Nontypeable Haemophilus influenzae Outer Membrane Vesicles Induces Cross-Protective Immunity in Mice
journal, August 2012
- Roier, Sandro; Leitner, Deborah R.; Iwashkiw, Jeremy
- PLoS ONE, Vol. 7, Issue 8
Pathogenicity and Immunity Studies of Haemophilus Parasuis Serotypes
journal, June 1993
- Nielsen, R.
- Acta Veterinaria Scandinavica, Vol. 34, Issue 2
Involvement of lipooligosaccharide heptose residues of Haemophilus parasuis SC096 strain in serum resistance, adhesion and invasion
journal, February 2013
- Xu, Chenggang; Zhang, Lingyun; Zhang, Bin
- The Veterinary Journal, Vol. 195, Issue 2
Virulence and Draft Genome Sequence Overview of Multiple Strains of the Swine Pathogen Haemophilus parasuis
journal, August 2014
- Brockmeier, Susan L.; Register, Karen B.; Kuehn, Joanna S.
- PLoS ONE, Vol. 9, Issue 8
Enterohemorrhagic Escherichia coli Hemolysin Employs Outer Membrane Vesicles to Target Mitochondria and Cause Endothelial and Epithelial Apoptosis
journal, December 2013
- Bielaszewska, Martina; Rüter, Christian; Kunsmann, Lisa
- PLoS Pathogens, Vol. 9, Issue 12
Protection of vaccinated pigs against experimental infections with homologous and heterologous Hoemophilus parasuis
journal, October 2002
- Bak, H.; Riising, H. -J.
- Veterinary Record, Vol. 151, Issue 17
Klebsiella pneumoniae secretes outer membrane vesicles that induce the innate immune response
journal, April 2012
- Lee, Je Chul; Lee, Eun Jeoung; Lee, Jung Hwa
- FEMS Microbiology Letters, Vol. 331, Issue 1
Immunogenicity and protective efficacy of recombinant Haemophilus parasuis SH0165 putative outer membrane proteins
journal, January 2013
- Fu, Shulin; Zhang, Minmin; Xu, Juan
- Vaccine, Vol. 31, Issue 2
Biogenesis and multifaceted roles of outer membrane vesicles from Gram-negative bacteria
journal, July 2014
- Kulkarni, H. M.; Jagannadham, M. V.
- Microbiology, Vol. 160, Issue Pt_10
Outer membrane vesicles alter inflammation and coagulation mediators
journal, November 2014
- Soult, Michael C.; Dobrydneva, Yuliya; Wahab, Kamal H.
- Journal of Surgical Research, Vol. 192, Issue 1
Proteomic analysis of Vibrio cholerae outer membrane vesicles
journal, March 2014
- Altindis, E.; Fu, Y.; Mekalanos, J. J.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 15
Naturally Produced Outer Membrane Vesicles from Pseudomonas aeruginosa Elicit a Potent Innate Immune Response via Combined Sensing of Both Lipopolysaccharide and Protein Components
journal, July 2010
- Ellis, T. N.; Leiman, S. A.; Kuehn, M. J.
- Infection and Immunity, Vol. 78, Issue 9
Intercellular Nanotubes Mediate Bacterial Communication
journal, February 2011
- Dubey, Gyanendra P.; Ben-Yehuda, Sigal
- Cell, Vol. 144, Issue 4, p. 590-600
Comparative proteome analysis of the extracellular proteins of two Haemophilus parasuis strains Nagasaki and SW114
journal, April 2014
- Wei, Xingliang; Cao, Sanjie; Zhang, Luhua
- Biochemical and Biophysical Research Communications, Vol. 446, Issue 4
Modification of the bicinchoninic acid protein assay to eliminate lipid interference in determining lipoprotein protein content
journal, August 1992
- Morton, Richard E.; Evans, Tracy A.
- Analytical Biochemistry, Vol. 204, Issue 2
CELLO2GO: A Web Server for Protein subCELlular LOcalization Prediction with Functional Gene Ontology Annotation
journal, June 2014
- Yu, Chin-Sheng; Cheng, Chih-Wen; Su, Wen-Chi
- PLoS ONE, Vol. 9, Issue 6
NIH Image to ImageJ: 25 years of image analysis
journal, June 2012
- Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W.
- Nature Methods, Vol. 9, Issue 7
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
journal, December 2001
- Livak, Kenneth J.; Schmittgen, Thomas D.
- Methods, Vol. 25, Issue 4
Characterization of the key antigenic components of pertussis vaccine based on outer membrane vesicles
journal, October 2014
- Ormazábal, Maximiliano; Bartel, Erika; Gaillard, María Emilia
- Vaccine, Vol. 32, Issue 46
Protein selection and export via outer membrane vesicles
journal, August 2014
- Bonnington, K. E.; Kuehn, M. J.
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1843, Issue 8
Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors
journal, November 2006
- Paoletti, A. C.; Parmely, T. J.; Tomomori-Sato, C.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 50
Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5
journal, August 2009
- Zhou, Mingguang; Guo, Yi; Zhao, Jianping
- Vaccine, Vol. 27, Issue 38
Outer Membrane Vesicles Mediate Transport of Biologically Active Vibrio cholerae Cytolysin (VCC) from V. cholerae Strains
journal, September 2014
- Elluri, Sridhar; Enow, Constance; Vdovikova, Svitlana
- PLoS ONE, Vol. 9, Issue 9
Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response
journal, August 2006
- Bauman, Susanne J.; Kuehn, Meta J.
- Microbes and Infection, Vol. 8, Issue 9-10
Gene Transfer Potential of Outer Membrane Vesicles of Acinetobacter baylyi and Effects of Stress on Vesiculation
journal, March 2014
- Fulsundar, Shweta; Harms, Klaus; Flaten, Gøril E.
- Applied and Environmental Microbiology, Vol. 80, Issue 11
A basis for vaccine development: Comparative characterization of Haemophilus influenzae outer membrane vesicles
journal, May 2015
- Roier, Sandro; Blume, Thomas; Klug, Lisa
- International Journal of Medical Microbiology, Vol. 305, Issue 3
LOS oligosaccharide modification enhances dendritic cell responses to meningococcal native outer membrane vesicles expressing a non-toxic lipid A: Dendritic cell responses to meningococcal vesicles
journal, November 2013
- Jones, Hannah E.; Copland, Alastair; Hamstra, Hendrik Jan
- Cellular Microbiology, Vol. 16, Issue 4
Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response
journal, August 2006
- Bauman, Susanne J.; Kuehn, Meta J.
- Microbes and Infection, Vol. 8, Issue 9-10
Protein selection and export via outer membrane vesicles
journal, August 2014
- Bonnington, K. E.; Kuehn, M. J.
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1843, Issue 8
Acinetobacter nosocomialis secretes outer membrane vesicles that induce epithelial cell death and host inflammatory responses
journal, April 2015
- Nho, Jae Su; Jun, So Hyun; Oh, Man Hwan
- Microbial Pathogenesis, Vol. 81
Trimeric Autotransporters of Haemophilus parasuis: Generation of an Extensive Passenger Domain Repertoire Specific for Pathogenic Strains
journal, November 2008
- Pina, S.; Olvera, A.; Barcelo, A.
- Journal of Bacteriology, Vol. 191, Issue 2
VtaA8 and VtaA9 from Haemophilus parasuis delay phagocytosis by alveolar macrophages
journal, January 2012
- Costa-Hurtado, Mar; Ballester, Maria; Galofré-Milà, Nuria
- Veterinary Research, Vol. 43, Issue 1
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
Global proteomic profiling of native outer membrane vesicles derived from Escherichia coli
journal, October 2007
- Lee, Eun-Young; Bang, Joo Young; Park, Gun Wook
- PROTEOMICS, Vol. 7, Issue 20
Innate Cytokine Responses in Porcine Macrophage Populations: Evidence for Differential Recognition of Double-Stranded RNA
journal, December 2006
- Loving, Crystal L.; Brockmeier, Susan L.; Ma, Wenjun
- The Journal of Immunology, Vol. 177, Issue 12
Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5
journal, March 2015
- Li, Miao; Song, Shuai; Yang, Dongxia
- Vaccine, Vol. 33, Issue 14
Effect of Different Vaccine Formulations on the Development of Glässer's Disease Induced in Pigs by Experimental Haemophilus parasuis Infection
journal, February 2009
- Martín de la Fuente, A. J.; Gutiérrez Martín, C. B.; Pérez Martínez, C.
- Journal of Comparative Pathology, Vol. 140, Issue 2-3
Virulence and Immunomodulatory Roles of Bacterial Outer Membrane Vesicles
journal, March 2010
- Ellis, T. N.; Kuehn, M. J.
- Microbiology and Molecular Biology Reviews, Vol. 74, Issue 1
PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes
journal, May 2010
- Yu, Nancy Y.; Wagner, James R.; Laird, Matthew R.
- Bioinformatics, Vol. 26, Issue 13
Protection of Piglets by a Haemophilus parasuis Ghost Vaccine against Homologous Challenge
journal, March 2013
- Hu, Mingming; Zhang, Yanhe; Xie, Fang
- Clinical and Vaccine Immunology, Vol. 20, Issue 6
Differences in phagocytosis susceptibility in Haemophilus parasuis strains
journal, February 2009
- Olvera, Alexandre; Ballester, Maria; Nofrarias, Miquel
- Veterinary Research, Vol. 40, Issue 3
Construction and immune effect of Haemophilus parasuis DNA vaccine encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in mice
journal, November 2012
- Fu, Shulin; Zhang, Minmin; Ou, Jiwen
- Vaccine, Vol. 30, Issue 48
Fusion of L egionella pneumophila outer membrane vesicles with eukaryotic membrane systems is a mechanism to deliver pathogen factors to host cell membranes : Membrane fusion of
journal, December 2014
- Jäger, Jens; Keese, Susanne; Roessle, Manfred
- Cellular Microbiology, Vol. 17, Issue 5
Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles
journal, October 2010
- Kulp, Adam; Kuehn, Meta J.
- Annual Review of Microbiology, Vol. 64, Issue 1
Biogenesis and multifaceted roles of outer membrane vesicles from Gram-negative bacteria
journal, July 2014
- Kulkarni, H. M.; Jagannadham, M. V.
- Microbiology, Vol. 160, Issue Pt_10
Global proteomic profiling of native outer membrane vesicles derived fromEscherichia coli
journal, September 2007
- Lee, Eun-Young; Bang, Joo Young; Park, Gun Wook
- PROTEOMICS, Vol. 7, Issue 17
Haemophilus parasuis exhibits IgA protease activity but lacks homologs of the IgA protease genes of Haemophilus influenzae
journal, December 2011
- Mullins, Michael A.; Register, Karen B.; Bayles, Darrell O.
- Veterinary Microbiology, Vol. 153, Issue 3-4
Immune modulation by bacterial outer membrane vesicles
journal, May 2015
- Kaparakis-Liaskos, Maria; Ferrero, Richard L.
- Nature Reviews Immunology, Vol. 15, Issue 6
Protection of vaccinated pigs against experimental infections with homologous and heterologous Hoemophilus parasuis
journal, October 2002
- Bak, H.; Riising, H. -J.
- Veterinary Record, Vol. 151, Issue 17
CELLO2GO: A Web Server for Protein subCELlular LOcalization Prediction with Functional Gene Ontology Annotation
journal, June 2014
- Yu, Chin-Sheng; Cheng, Chih-Wen; Su, Wen-Chi
- PLoS ONE, Vol. 9, Issue 6
Proteomic analysis of Vibrio cholerae outer membrane vesicles
journal, March 2014
- Altindis, E.; Fu, Y.; Mekalanos, J. J.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 15
Outer Membrane Vesicles Mediate Transport of Biologically Active Vibrio cholerae Cytolysin (VCC) from V. cholerae Strains
journal, September 2014
- Elluri, Sridhar; Enow, Constance; Vdovikova, Svitlana
- PLoS ONE, Vol. 9, Issue 9
Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles
journal, October 2010
- Kulp, Adam; Kuehn, Meta J.
- Annual Review of Microbiology, Vol. 64, Issue 1
Differences in phagocytosis susceptibility in Haemophilus parasuis strains
journal, February 2009
- Olvera, Alexandre; Ballester, Maria; Nofrarias, Miquel
- Veterinary Research, Vol. 40, Issue 3
Properties and clinical performance of vaccines containing outer membrane vesicles from Neisseria meningitidis
journal, June 2009
- Holst, Johan; Martin, Diana; Arnold, Richard
- Vaccine, Vol. 27
Shewanella oneidensis MR-1 nanowires are outer membrane and periplasmic extensions of the extracellular electron transport components
journal, August 2014
- Pirbadian, S.; Barchinger, S. E.; Leung, K. M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 35
Update on the diagnosis of Haemophilus parasuis infection in pigs and novel genotyping methods
journal, November 2007
- Olvera, Alex; Segalés, Joaquim; Aragón, Virginia
- The Veterinary Journal, Vol. 174, Issue 3
Intranasal Immunization with Nontypeable Haemophilus influenzae Outer Membrane Vesicles Induces Cross-Protective Immunity in Mice
journal, August 2012
- Roier, Sandro; Leitner, Deborah R.; Iwashkiw, Jeremy
- PLoS ONE, Vol. 7, Issue 8
A comprehensive proteome map of the Haemophilus parasuis serovar 5
journal, May 2009
- Zhou, Mingguang; Zhang, Anding; Guo, Yi
- PROTEOMICS, Vol. 9, Issue 10
NIH Image to ImageJ: 25 years of image analysis
journal, June 2012
- Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W.
- Nature Methods, Vol. 9, Issue 7
Polysaccharide biosynthesis protein CapD is a novel pathogenicity-associated determinant of Haemophilus parasuis involved in serum-resistance ability
journal, May 2013
- Wang, Xiangru; Xu, Xiaojuan; Wu, Yucong
- Veterinary Microbiology, Vol. 164, Issue 1-2
Cytolethal distending toxin (CDT) of the Haemophilus parasuis SC096 strain contributes to serum resistance and adherence to and invasion of PK-15 and PUVEC cells
journal, May 2012
- Zhang, Bin; He, Yanbing; Xu, Chenggang
- Veterinary Microbiology, Vol. 157, Issue 1-2
Klebsiella pneumoniae secretes outer membrane vesicles that induce the innate immune response
journal, April 2012
- Lee, Je Chul; Lee, Eun Jeoung; Lee, Jung Hwa
- FEMS Microbiology Letters, Vol. 331, Issue 1
Long-Distance Delivery of Bacterial Virulence Factors by Pseudomonas aeruginosa Outer Membrane Vesicles
journal, April 2009
- Bomberger, Jennifer M.; MacEachran, Daniel P.; Coutermarsh, Bonita A.
- PLoS Pathogens, Vol. 5, Issue 4
Purification and renaturation of membrane neuraminidase from Haemophilus parasuis
journal, May 2003
- Lichtensteiger, Carol A.; Vimr, Eric R.
- Veterinary Microbiology, Vol. 93, Issue 1
Elicitation of Epithelial Cell-Derived Immune Effectors by Outer Membrane Vesicles of Nontypeable Haemophilus influenzae
journal, August 2011
- Sharpe, Samantha W.; Kuehn, Meta J.; Mason, Kevin M.
- Infection and Immunity, Vol. 79, Issue 11
Diversion of the host humoral response: a novel virulence mechanism of Haemophilus influenzae mediated via outer membrane vesicles
journal, June 2014
- Deknuydt, Florence; Nordström, Therése; Riesbeck, Kristian
- Journal of Leukocyte Biology, Vol. 95, Issue 6
Virulence and Draft Genome Sequence Overview of Multiple Strains of the Swine Pathogen Haemophilus parasuis
journal, August 2014
- Brockmeier, Susan L.; Register, Karen B.; Kuehn, Joanna S.
- PLoS ONE, Vol. 9, Issue 8
Effect of Different Vaccine Formulations on the Development of Glässer's Disease Induced in Pigs by Experimental Haemophilus parasuis Infection
journal, February 2009
- Martín de la Fuente, A. J.; Gutiérrez Martín, C. B.; Pérez Martínez, C.
- Journal of Comparative Pathology, Vol. 140, Issue 2-3
Involvement of lipooligosaccharide heptose residues of Haemophilus parasuis SC096 strain in serum resistance, adhesion and invasion
journal, February 2013
- Xu, Chenggang; Zhang, Lingyun; Zhang, Bin
- The Veterinary Journal, Vol. 195, Issue 2
Haemophilus parasuis: new trends on diagnosis, epidemiology and control
journal, March 2004
- Oliveira, Simone; Pijoan, Carlos
- Veterinary Microbiology, Vol. 99, Issue 1
Pentavalent outer membrane vesicles of Vibrio cholerae induce adaptive immune response and protective efficacy in both adult and passive suckling mice models
journal, March 2015
- Sinha, Ritam; Koley, Hemanta; Nag, Dhrubajyoti
- Microbes and Infection, Vol. 17, Issue 3
Pentavalent outer membrane vesicles of Vibrio cholerae induce adaptive immune response and protective efficacy in both adult and passive suckling mice models
journal, March 2015
- Sinha, Ritam; Koley, Hemanta; Nag, Dhrubajyoti
- Microbes and Infection, Vol. 17, Issue 3
The Live Attenuated Actinobacillus pleuropneumoniae Triple-Deletion Mutant Δ apxIC Δ apxIIC Δ apxIV - ORF1 Strain, SLW05, Immunizes Pigs against Lethal Challenge with Haemophilus parasuis
journal, December 2012
- Fu, Shulin; Ou, Jiwen; Zhang, Minmin
- Clinical and Vaccine Immunology, Vol. 20, Issue 2
Comparative proteome analysis of the extracellular proteins of two Haemophilus parasuis strains Nagasaki and SW114
journal, April 2014
- Wei, Xingliang; Cao, Sanjie; Zhang, Luhua
- Biochemical and Biophysical Research Communications, Vol. 446, Issue 4
Fusion of L egionella pneumophila outer membrane vesicles with eukaryotic membrane systems is a mechanism to deliver pathogen factors to host cell membranes : Membrane fusion of
journal, December 2014
- Jäger, Jens; Keese, Susanne; Roessle, Manfred
- Cellular Microbiology, Vol. 17, Issue 5
Coinfection of pigs with porcine respiratory coronavirus and Bordetella bronchiseptica
journal, April 2008
- Brockmeier, S. L.; Loving, C. L.; Nicholson, T. L.
- Veterinary Microbiology, Vol. 128, Issue 1-2
Helicobacter pylori Outer Membrane Vesicles Modulate Proliferation and Interleukin-8 Production by Gastric Epithelial Cells
journal, September 2003
- Ismail, S.; Hampton, M. B.; Keenan, J. I.
- Infection and Immunity, Vol. 71, Issue 10
New Type of Outer Membrane Vesicle Produced by the Gram-Negative Bacterium Shewanella vesiculosa M7 T : Implications for DNA Content
journal, January 2013
- Pérez-Cruz, Carla; Carrión, Ornella; Delgado, Lidia
- Applied and Environmental Microbiology, Vol. 79, Issue 6
Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors
journal, November 2006
- Paoletti, A. C.; Parmely, T. J.; Tomomori-Sato, C.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 50
A Cross-Protection Experiment in Pigs Vaccinated with Haemophilus parasuis Serovars 2 and 5 Bacterins, and Evaluation of a Bivalent Vaccine under Laboratory and Field Conditions.
journal, January 2001
- Takahashi, Kinya; Nagai, Shinya; Yagihashi, Takeshi
- Journal of Veterinary Medical Science, Vol. 63, Issue 5
Construction and immune effect of Haemophilus parasuis DNA vaccine encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in mice
journal, November 2012
- Fu, Shulin; Zhang, Minmin; Ou, Jiwen
- Vaccine, Vol. 30, Issue 48
Properties and clinical performance of vaccines containing outer membrane vesicles from Neisseria meningitidis
journal, June 2009
- Holst, Johan; Martin, Diana; Arnold, Richard
- Vaccine, Vol. 27
Cytokine expression in colostrum-deprived pigs immunized and challenged with Haemophilus parasuis
journal, August 2009
- Fuente, A. J. Martín de la; Ferri, E. F. Rodríguez; Tejerina, F.
- Research in Veterinary Science, Vol. 87, Issue 1
Haemophilus parasuis: new trends on diagnosis, epidemiology and control
journal, March 2004
- Oliveira, Simone; Pijoan, Carlos
- Veterinary Microbiology, Vol. 99, Issue 1
Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5
journal, March 2015
- Li, Miao; Song, Shuai; Yang, Dongxia
- Vaccine, Vol. 33, Issue 14
Acinetobacter nosocomialis secretes outer membrane vesicles that induce epithelial cell death and host inflammatory responses
journal, April 2015
- Nho, Jae Su; Jun, So Hyun; Oh, Man Hwan
- Microbial Pathogenesis, Vol. 81
Enterohemorrhagic Escherichia coli Hemolysin Employs Outer Membrane Vesicles to Target Mitochondria and Cause Endothelial and Epithelial Apoptosis
journal, December 2013
- Bielaszewska, Martina; Rüter, Christian; Kunsmann, Lisa
- PLoS Pathogens, Vol. 9, Issue 12
Innate Cytokine Responses in Porcine Macrophage Populations: Evidence for Differential Recognition of Double-Stranded RNA
journal, December 2006
- Loving, Crystal L.; Brockmeier, Susan L.; Ma, Wenjun
- The Journal of Immunology, Vol. 177, Issue 12
Diversion of the host humoral response: a novel virulence mechanism of Haemophilus influenzae mediated via outer membrane vesicles
journal, June 2014
- Deknuydt, Florence; Nordström, Therése; Riesbeck, Kristian
- Journal of Leukocyte Biology, Vol. 95, Issue 6
A basis for vaccine development: Comparative characterization of Haemophilus influenzae outer membrane vesicles
journal, May 2015
- Roier, Sandro; Blume, Thomas; Klug, Lisa
- International Journal of Medical Microbiology, Vol. 305, Issue 3
Intercellular Nanotubes Mediate Bacterial Communication
journal, February 2011
- Dubey, Gyanendra P.; Ben-Yehuda, Sigal
- Cell, Vol. 144, Issue 4, p. 590-600
VtaA8 and VtaA9 from Haemophilus parasuis delay phagocytosis by alveolar macrophages
journal, January 2012
- Costa-Hurtado, Mar; Ballester, Maria; Galofré-Milà, Nuria
- Veterinary Research, Vol. 43, Issue 1
Virulence, Transmission, and Heterologous Protection of Four Isolates of Haemophilus parasuis
journal, July 2013
- Brockmeier, Susan L.; Loving, Crystal L.; Mullins, Michael A.
- Clinical and Vaccine Immunology, Vol. 20, Issue 9
Production of Outer Membrane Vesicles and Outer Membrane Tubes by Francisella novicida
journal, December 2012
- McCaig, W. D.; Koller, A.; Thanassi, D. G.
- Journal of Bacteriology, Vol. 195, Issue 6
Works referencing / citing this record:
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journal, December 2018
- Cai, Wei; Kesavan, Dinesh Kumar; Wan, Jie
- Diagnostic Pathology, Vol. 13, Issue 1
Epidemiological and PK/PD cutoff values determination and PK/PD-based dose assessment of gamithromycin against Haemophilus parasuis in piglets
journal, March 2020
- Zhou, Yu-Feng; Bu, Ming-Xiao; Liu, Ping
- BMC Veterinary Research, Vol. 16, Issue 1
The Therapeutic Benefit of Bacterial Membrane Vesicles
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- Bitto, Natalie; Kaparakis-Liaskos, Maria
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Extracellular Membrane Vesicles from Lactobacilli Dampen IFN-γ Responses in a Monocyte-Dependent Manner
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- Martins, Paula; Machado, Daisy; Theizen, Thais Holtz
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Environmentally controlled bacterial vesicle-mediated export: Environmentally controlled bacterial vesicle-mediated export
journal, October 2016
- Orench-Rivera, Nichole; Kuehn, Meta J.
- Cellular Microbiology, Vol. 18, Issue 11
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- Mathieu-Denoncourt, Annabelle; Letendre, Corinne; Auger, Jean-Philippe
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- Dai, Ke; Ma, Xiaoyu; Yang, Zhen
- Vaccines, Vol. 7, Issue 4
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- Mathieu-Denoncourt, Annabelle; Letendre, Corinne; Auger, Jean-Philippe
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journal, March 2019
- Cheng, Likun; Li, Feng; Li, Shuguang
- Biotechnology Progress, Vol. 35, Issue 3
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- Martins, Paula; Machado, Daisy; Theizen, Thais Holtz
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Development of Serotype-Specific PCR Assays for Typing of Haemophilus parasuis Isolates Circulating in Southern China
journal, September 2017
- Jia, Aiqing; Zhou, Ruyue; Fan, Huiying
- Journal of Clinical Microbiology, Vol. 55, Issue 11
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journal, June 2017
- Bitto, Natalie; Kaparakis-Liaskos, Maria
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