skip to main content


Title: Assessing the use of reflectance spectroscopy in determining CsCl stress in the model species Arabidopsis thaliana

Here, reflectance spectroscopy is a rapid and non-destructive analytical technique that may be used for assessing plant stress, and has potential applications for use in remediation. Changes in reflectance such as that due to metal stress may occur before damage is visible, and existing studies have shown that metal stress does cause changes in plant reflectance. To further investigate the potential use of reflectance spectroscopy as a method for assessing metal stress in plants, an exploratory study was conducted in which Arabidopsis thaliana plants were treated twice weekly in a laboratory setting with varying levels (0, 0.5, or 5 mM (millimolar)) of caesium chloride (CsCl) solution, and reflectance spectra were collected every week for three weeks using an Analytical Spectral Devices FieldSpec Pro spectroradiometer with both a contact probe (CP) and a field of view (FOV) probe at 36.8 and 66.7 cm, respectively, above the plant. Plants were harvested each week after spectra collection for determination of relative water content and chlorophyll content. A visual assessment of the plants was also conducted using point observations on a uniform grid of 81 points. A mixed-effects model analysis was conducted for each vegetation index (VI) considered to determine the effects of lengthmore » of treatment, treatment level, view with which spectra were acquired, and the interactions of these terms. Two-way analyses of variance (ANOVAs) were performed on the aforementioned endpoints (e.g. chlorophyll content) to determine the significance of the effects of treatment level and length of treatment. Multiple linear regression (MLR) was used to develop a predictive model for each endpoint, considering VI acquired at each view (CP, high FOV, and low FOV). Of the 14 VI considered, 8 were included in the MLR models. Contact probe readings and FOV readings differed significantly, but FOV measurements were generally consistent at each height.« less
 [1] ;  [2] ;  [3] ;  [4] ;  [3] ;  [3]
  1. Clemson Univ., Clemson, SC (United States); Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)
  2. Clemson Univ., Clemson, SC (United States)
  3. Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)
  4. Colorado State Univ., Fort Collins, CO (United States)
Publication Date:
Report Number(s):
Journal ID: ISSN 0143-1161
Grant/Contract Number:
Accepted Manuscript
Journal Name:
International Journal of Remote Sensing
Additional Journal Information:
Journal Volume: 36; Journal Issue: 23; Journal ID: ISSN 0143-1161
Taylor & Francis
Research Org:
Savannah River Site (SRS), Aiken, SC (United States)
Sponsoring Org:
USDOE National Nuclear Security Administration (NNSA)
Country of Publication:
United States
47 OTHER INSTRUMENTATION; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; reflectance spectroscopy; remote sensing; caesium; Arabidopsis thaliana
OSTI Identifier: