Activity-based protein profiling of microbes

Sadler, Natalie C.; Wright, Aaron T.

doi:10.1016/j.cbpa.2014.10.022

Title: Activity-based protein profiling of microbes

Full Record
Other Related Research

Authors:: Sadler, Natalie C.; Wright, Aaron T.

Publication Date:: Sun Feb 01 00:00:00 EST 2015

Sponsoring Org.:: USDOE

OSTI Identifier:: 1251453

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Current Opinion in Chemical Biology

Additional Journal Information:: Journal Name: Current Opinion in Chemical Biology Journal Volume: 24 Journal Issue: C; Journal ID: ISSN 1367-5931

Publisher:: Elsevier

Country of Publication:: Netherlands

Language:: English

Citation Formats


                    Sadler, Natalie C., and Wright, Aaron T. Activity-based protein profiling of microbes.  Netherlands: N. p., 2015. 
Web.  doi:10.1016/j.cbpa.2014.10.022.

Copy to clipboard


                    Sadler, Natalie C., & Wright, Aaron T. Activity-based protein profiling of microbes.  Netherlands.  https://doi.org/10.1016/j.cbpa.2014.10.022

Copy to clipboard


                    Sadler, Natalie C., and Wright, Aaron T. Sun .  
"Activity-based protein profiling of microbes".  Netherlands.  https://doi.org/10.1016/j.cbpa.2014.10.022.

Copy to clipboard


                    
@article{osti_1251453,

  title        = {Activity-based protein profiling of microbes},

  author       = {Sadler, Natalie C. and Wright, Aaron T.},

  abstractNote = {},

  doi          = {10.1016/j.cbpa.2014.10.022},

  journal      = {Current Opinion in Chemical Biology},

  number       = C,

  volume       = 24,

  place        = {Netherlands},

  year         = {Sun Feb 01 00:00:00 EST 2015},

  month        = {Sun Feb 01 00:00:00 EST 2015}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (Publisher)

Publisher's Version of Record
https://doi.org/10.1016/j.cbpa.2014.10.022

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 44 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

Activity-Based Protein Profiling of Microbes

Journal Article Sadler, Natalie C. ; Wright, Aaron T. - Current Opinion in Chemical Biology, 24:139-144

Activity-Based Protein Profiling (ABPP) in conjunction with multimodal characterization techniques has yielded impactful findings in microbiology, particularly in pathogen, bioenergy, drug discovery, and environmental research. Using small molecule chemical probes that react irreversibly with specific proteins or protein families in complex systems has provided insights in enzyme functions in central metabolic pathways, drug-protein interactions, and regulatory protein redox, for systems ranging from photoautotrophic cyanobacteria to mycobacteria, and combining live cell or cell extract ABPP with proteomics, molecular biology, modeling, and other techniques has greatly expanded our understanding of these systems. New opportunities for application of ABPP to microbial systems include:more »« less
https://doi.org/10.1016/j.cbpa.2014.10.022
Plasma Protein Turnover Rates in Rats Using Stable Isotope Labeling, Global Proteomics, and Activity-Based Protein Profiling

Journal Article Smith, Jordan Ned ; Tyrrell, Kimberly J. ; Hansen, Joshua R. ; ... - Analytical Chemistry

Protein turnover is important for general health on cellular and organism scales providing a strategy to replace old, damaged, or dysfunctional proteins. Protein turnover also informs of biomarker kinetics, as a better understanding of synthesis and degradation of proteins increases the clinical utility of biomarkers. Here, turnover rates of plasma proteins in rats were measured in vivo using a pulse-chase stable isotope labeling experiment. During the pulse, rats (n=5) were fed 13C6-labeled lysine (“heavy”) feed for 23 days to label proteins. During the chase, feed was changed to an unlabeled equivalent feed (“light”), and blood was repeatedly sampled from ratsmore »« less
https://doi.org/10.1021/acs.analchem.7b03984
Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30

Journal Article Anderson, Lindsey N. ; Culley, David E. ; Hofstad, Beth A. ; ... - Molecular Biosystems, 9(12):2992-3000

Development of alternative, non-petroleum based sources of bioenergy that can be applied in the short-term find great promise in the use of highly abundant and renewable lignocellulosic plant biomass.1 This material obtained from different feedstocks, such as forest litter or agricultural residues, can yield liquid fuels and other chemical products through biorefinery processes.2 Biofuels are obtained from lignocellulosic materials by chemical pretreatment of the biomass, followed by enzymatic decomposition of cellulosic and hemicellulosic compounds into soluble sugars that are converted to desired chemical products via microbial metabolism and fermentation.3, 4 To release soluble sugars from polymeric cellulose multiple enzymes aremore »« less
https://doi.org/10.1039/c3mb70333a
Activity-based protein profiling identifies alternating activation of enzymes involved in the bifidobacterium shunt pathway or mucin degradation in the gut microbiome response to soluble dietary fiber

Journal Article Killinger, Bryan J. ; Whidbey, Christopher-Mychael ; Sadler, Natalie C. ; ... - npj Biofilms and Microbiomes

While deprivation of dietary fiber has been associated with adverse health outcomes, investigations concerning the effect of dietary fiber on the gut microbiome have been largely limited to compositional sequence-based analyses or utilize a defined microbiota not native to the host. To extend understanding of the microbiome’s functional response to dietary fiber deprivation beyond correlative evidence from sequence-based analyses, approaches capable of measuring functional enzymatic activity are needed. In this study, we use an activity-based protein profiling (ABPP) approach to identify sugar metabolizing and transport proteins in native mouse gut microbiomes that respond with differential activity to the deprivation ormore »« less
https://doi.org/10.1038/s41522-022-00313-z

Full Text Available
Activity-based protein profiling of chitin catabolism

Journal Article Zegeye, Elias ; Sadler, Natalie C. ; Lomas, Gerard X. ; ... - Chembiochem

Microbial catabolism of chitin, a highly abundant component of polymeric organic matter worldwide, is a fundamental cog in the carbon and nitrogen cycles that govern CO2 emissions, nutrient dynamics in aquatic systems, and soil vitality. Despite this critical biogeochemical function, we have a poor understanding of the synergy between various microbial chitinases and key accessory enzymes involved in chitin catabolism. We designed and synthesized activity-based probes (ABPs) for profiling active chitinolytic enzymes by modifying the chitin derivatives N-acetyl glucosamine and chitotriose. The ABPs were used to determine the active complement of chitinolytic enzymes produced over time by the soil bacteriummore »« less
https://doi.org/10.1002/cbic.202000616

Similar Records