Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium
Abstract
Escherichia coli KJ122 was engineered to produce succinate from glucose using the wild type GalP for glucose uptake instead of the native phosphotransferase system (ptsI mutation). This strain now ferments 10% xylose poorly. Mutants were selected by serial transfers in AM1 mineral salts medium with 10% xylose. Clones from this population all exhibited a similar improvement, co-fermentation of an equal mixture of xylose and glucose. One of these, AS1600a, produced 84.26 ± 1.37 g/L succinate, equivalent to that produced by the parent (KJ122) from 10% glucose (85.46 ± 1.78 g/L). AS1600a was sequenced and found to contain a mutation in galactose permease (GalP, G236D). This mutation was shown to be responsible for the improvement in fermentation using KJΔgalP as the host and expression vectors with native galP and with mutant galP*. Strain AS1600a and KJΔgalP(pLOI5746; galP*) also co-fermented a mixture of glucose, xylose, arabinose, and galactose in sugarcane bagasse hydrolysate using mineral salts medium.
- Authors:
-
- Suranaree Univ. of Technology, Nakhon Ratchasima (Thailand)
- Univ. of Florida, Gainesville, FL (United States)
- Publication Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE Office of International Affairs (IA); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; U.S. Department of Agriculture; Thailand Research Fund (TRF)
- OSTI Identifier:
- 1771326
- Alternate Identifier(s):
- OSTI ID: 1250363; OSTI ID: 1661478
- Grant/Contract Number:
- PI0000031; PHD/0135/2553
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Bioresource Technology
- Additional Journal Information:
- Journal Volume: 193; Journal ID: ISSN 0960-8524
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Succinate; Furfural; Sugarcane bagasse; Xylose; Arabinose; succinate; furfural; sugarcane bagasse; xylose; arabinose
Citation Formats
Sawisit, Apichai, Jantama, Kaemwich, Zheng, Huabao, Yomano, Lorraine P., York, Sean W., Shanmugam, Keelnatham T., and Ingram, Lonnie O. Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium. United States: N. p., 2015.
Web. doi:10.1016/j.biortech.2015.06.108.
Sawisit, Apichai, Jantama, Kaemwich, Zheng, Huabao, Yomano, Lorraine P., York, Sean W., Shanmugam, Keelnatham T., & Ingram, Lonnie O. Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium. United States. https://doi.org/10.1016/j.biortech.2015.06.108
Sawisit, Apichai, Jantama, Kaemwich, Zheng, Huabao, Yomano, Lorraine P., York, Sean W., Shanmugam, Keelnatham T., and Ingram, Lonnie O. Thu .
"Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium". United States. https://doi.org/10.1016/j.biortech.2015.06.108. https://www.osti.gov/servlets/purl/1771326.
@article{osti_1771326,
title = {Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium},
author = {Sawisit, Apichai and Jantama, Kaemwich and Zheng, Huabao and Yomano, Lorraine P. and York, Sean W. and Shanmugam, Keelnatham T. and Ingram, Lonnie O.},
abstractNote = {Escherichia coli KJ122 was engineered to produce succinate from glucose using the wild type GalP for glucose uptake instead of the native phosphotransferase system (ptsI mutation). This strain now ferments 10% xylose poorly. Mutants were selected by serial transfers in AM1 mineral salts medium with 10% xylose. Clones from this population all exhibited a similar improvement, co-fermentation of an equal mixture of xylose and glucose. One of these, AS1600a, produced 84.26 ± 1.37 g/L succinate, equivalent to that produced by the parent (KJ122) from 10% glucose (85.46 ± 1.78 g/L). AS1600a was sequenced and found to contain a mutation in galactose permease (GalP, G236D). This mutation was shown to be responsible for the improvement in fermentation using KJΔgalP as the host and expression vectors with native galP and with mutant galP*. Strain AS1600a and KJΔgalP(pLOI5746; galP*) also co-fermented a mixture of glucose, xylose, arabinose, and galactose in sugarcane bagasse hydrolysate using mineral salts medium.},
doi = {10.1016/j.biortech.2015.06.108},
journal = {Bioresource Technology},
number = ,
volume = 193,
place = {United States},
year = {Thu Jun 25 00:00:00 EDT 2015},
month = {Thu Jun 25 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
Efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered Escherichia coli
journal, December 2013
- Liu, Rongming; Liang, Liya; Li, Feng
- Bioresource Technology, Vol. 149
Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation
journal, January 2011
- Wang, Dan; Li, Qiang; Yang, Maohua
- Process Biochemistry, Vol. 46, Issue 1
Metabolic engineering of Enterobacter cloacae for high-yield production of enantiopure (2 R ,3 R )-2,3-butanediol from lignocellulose-derived sugars
journal, March 2015
- Li, Lixiang; Li, Kun; Wang, Yu
- Metabolic Engineering, Vol. 28
Detoxification of Lignocellulose Hydrolysates: Biochemical and Metabolic Engineering Toward White Biotechnology
journal, August 2012
- Chandel, Anuj K.; da Silva, Silvio Silvério; Singh, Om V.
- BioEnergy Research, Vol. 6, Issue 1
Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals
journal, February 2013
- Wang, X.; Yomano, L. P.; Lee, J. Y.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 10, p. 4021-4026
Low salt medium for lactate and ethanol production by recombinant Escherichia coli B
journal, December 2006
- Martinez, Alfredo; Grabar, T. B.; Shanmugam, K. T.
- Biotechnology Letters, Vol. 29, Issue 3, p. 397-404
Succinic acid production from corn stalk hydrolysate in an E. coli mutant generated by atmospheric and room-temperature plasmas and metabolic evolution strategies
journal, October 2013
- Jiang, Min; Wan, Qing; Liu, Rongming
- Journal of Industrial Microbiology & Biotechnology, Vol. 41, Issue 1
The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli
journal, January 2011
- Archer, Colin T.; Kim, Jihyun F.; Jeong, Haeyoung
- BMC Genomics, Vol. 12, Issue 1
Effect of Different Carbon Sources on the Production of Succinic Acid Using Metabolically Engineered Escherichia coli
journal, April 2007
- Andersson, C.; Hodge, D.; Berglund, K. A.
- Biotechnology Progress, Vol. 23, Issue 2
Wood hydrolyzate treatments for improved fermentation of wood sugars to 2,3-butanediol
journal, January 1989
- Frazer, F. R.; McCaskey, T. A.
- Biomass, Vol. 18, Issue 1
Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180
journal, July 2014
- Geddes, Ryan D.; Wang, Xuan; Yomano, Lorraine P.
- Applied and Environmental Microbiology, Vol. 80, Issue 19
The Prototypical H+/Galactose Symporter GalP Assembles into Functional Trimers
journal, February 2010
- Zheng, Hongjin; Taraska, Justin; Merz, Alexey J.
- Journal of Molecular Biology, Vol. 396, Issue 3
Biotechnology of succinic acid production and markets for derived industrial products
journal, May 1999
- Zeikus, J. G.; Jain, M. K.; Elankovan, P.
- Applied Microbiology and Biotechnology, Vol. 51, Issue 5
Expression of galP and glk in a Escherichia coli PTS mutant restores glucose transport and increases glycolytic flux to fermentation products
journal, June 2003
- Hernández-Montalvo, Verónica; Martínez, Alfredo; Hernández-Chavez, Georgina
- Biotechnology and Bioengineering, Vol. 83, Issue 6
Effects of Ca(OH)2 treatments (“overliming”) on the composition and toxicity of bagasse hemicellulose hydrolysates
journal, January 2000
- Martinez, Alfredo; Rodriguez, Maria E.; York, Sean W.
- Biotechnology and Bioengineering, Vol. 69, Issue 5, p. 526-536
Seed train development for the fermentation of bagasse from sweet sorghum and sugarcane using a simplified fermentation process
journal, January 2013
- Geddes, C. C.; Mullinnix, M. T.; Nieves, I. U.
- Bioresource Technology, Vol. 128
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000
- Datsenko, K. A.; Wanner, B. L.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
Re-engineering Escherichia coli for ethanol production
journal, September 2008
- Yomano, L. P.; York, S. W.; Zhou, S.
- Biotechnology Letters, Vol. 30, Issue 12
Furfural Inhibits Growth by Limiting Sulfur Assimilation in Ethanologenic Escherichia coli Strain LY180
journal, August 2009
- Miller, E. N.; Jarboe, L. R.; Turner, P. C.
- Applied and Environmental Microbiology, Vol. 75, Issue 19
Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli
journal, November 2009
- Zhang, X.; Jantama, K.; Moore, J. C.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 48, p. 20180-20185
Succinic acid production from hemicellulose hydrolysate by an Escherichia coli mutant obtained by atmospheric and room temperature plasma and adaptive evolution
journal, November 2014
- Bao, Haijiao; Liu, Rongming; Liang, Liya
- Enzyme and Microbial Technology, Vol. 66
Combining treatments to improve the fermentation of sugarcane bagasse hydrolysates by ethanologenic Escherichia coli LY180
journal, August 2015
- Geddes, Ryan; Shanmugam, Keelnatham T.; Ingram, Lonnie O.
- Bioresource Technology, Vol. 189
Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C
journal, December 2008
- Jantama, Kaemwich; Zhang, Xueli; Moore, J. C.
- Biotechnology and Bioengineering, Vol. 101, Issue 5, p. 881-893
Effect of reduced sulfur compounds on the fermentation of phosphoric acid pretreated sugarcane bagasse by ethanologenic Escherichia coli
journal, April 2011
- Nieves, I. U.; Geddes, C. C.; Miller, E. N.
- Bioresource Technology, Vol. 102, Issue 8
Repetitive succinic acid production from lignocellulose hydrolysates by enhancement of ATP supply in metabolically engineered Escherichia coli
journal, September 2013
- Liang, Liya; Liu, Rongming; Li, Feng
- Bioresource Technology, Vol. 143
Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source
journal, May 2013
- Liu, Rongming; Liang, Liya; Cao, Weijia
- Bioresource Technology, Vol. 135
Engineering and adaptive evolution of Escherichia coli for d-lactate fermentation reveals GatC as a xylose transporter
journal, September 2012
- Utrilla, José; Licona-Cassani, Cuauhtemoc; Marcellin, Esteban
- Metabolic Engineering, Vol. 14, Issue 5
Aromatic inhibitors derived from ammonia-pretreated lignocellulose hinder bacterial ethanologenesis by activating regulatory circuits controlling inhibitor efflux and detoxification
journal, August 2014
- Keating, David H.; Zhang, Yaoping; Ong, Irene M.
- Frontiers in Microbiology, Vol. 5
Production of Optically Pure D-Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110
journal, January 2003
- Zhou, S.; Causey, T. B.; Hasona, A.
- Applied and Environmental Microbiology, Vol. 69, Issue 1
Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
journal, January 2008
- Jantama, Kaemwich; Haupt, M. J.; Svoronos, Spyros A.
- Biotechnology and Bioengineering, Vol. 99, Issue 5, p. 1140-1153
Works referencing / citing this record:
Bio‐based succinic acid: an overview of strain development, substrate utilization, and downstream purification
journal, November 2019
- Dai, Zhongxue; Guo, Feng; Zhang, Shangjie
- Biofuels, Bioproducts and Biorefining, Vol. 14, Issue 5
ATP-Based Ratio Regulation of Glucose and Xylose Improved Succinate Production
journal, June 2016
- Zhang, Fengyu; Li, Jiaojiao; Liu, Huaiwei
- PLOS ONE, Vol. 11, Issue 6
Experimental evolution reveals an effective avenue to release catabolite repression via mutations in XylR
journal, June 2017
- Sievert, Christian; Nieves, Lizbeth M.; Panyon, Larry A.
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 28
Chromosomal mutations in Escherichia coli that improve tolerance to nonvolatile side‐products from dilute acid treatment of sugarcane bagasse
journal, November 2019
- Shi, Aiqin; Yomano, Lorraine P.; York, Sean W.
- Biotechnology and Bioengineering, Vol. 117, Issue 1
Parallel experimental evolution reveals a novel repressive control of GalP on xylose fermentation in Escherichia coli
journal, May 2019
- Kurgan, Gavin; Sievert, Christian; Flores, Andrew
- Biotechnology and Bioengineering, Vol. 116, Issue 8
ATP-Based Ratio Regulation of Glucose and Xylose Improved Succinate Production
journal, June 2016
- Zhang, Fengyu; Li, Jiaojiao; Liu, Huaiwei
- PLOS ONE, Vol. 11, Issue 6
Catabolic Division of Labor Enhances Production of D-Lactate and Succinate From Glucose-Xylose Mixtures in Engineered Escherichia coli Co-culture Systems
journal, May 2020
- Flores, Andrew D.; Choi, Hyun G.; Martinez, Rodrigo
- Frontiers in Bioengineering and Biotechnology, Vol. 8
Short-Term Adaptation Modulates Anaerobic Metabolic Flux to Succinate by Activating ExuT, a Novel D-Glucose Transporter in Escherichia coli
journal, January 2020
- Kim, Hyun Ju; Jeong, Haeyoung; Lee, Sang Jun
- Frontiers in Microbiology, Vol. 11