1α,25-dihydroxyvitamin D3 stimulates human SOST gene expression and sclerostin secretion
Abstract
Sclerostin, the SOST gene product, is a negative regulator of bone formation and a positive regulator of bone resorption. Here, in this study, treatment of human primary osteoblasts, including cells differentiated to an osteocyte-like stage, with 1α,25-dihydroxyvitaminD3 (1,25D) resulted in the dose-dependent increased expression of SOST mRNA. A similar effect was observed in human trabecular bone samples cultured ex vivo, and in osteocyte-like cultures of differentiated SAOS2 cells. Treatment of SAOS2 cells with 1,25D resulted in the production and secretion of sclerostin protein. In silico analysis of the human SOST gene revealed a single putative DR3-type vitamin D response element (VDRE) at position -6216 bp upstream of the transcription start site (TSS). This sequence was confirmed to have strong VDRE activity by luciferase reporter assays and electrophoretic mobility shift analysis (EMSA). Sequence substitution in the VDR/RXR half-sites abolished VDRE reporter activity and binding of nuclear proteins. A 6.3 kb fragment of the human proximal SOST promoter demonstrated responsiveness to 1,25D. The addition of the evolutionary conserved region 5 (ECR5), a known bone specific enhancer region, ahead of the 6.3 kb fragment increased basal promoter activity but did not increase 1,25D responsiveness. Site-specific mutagenesis abolished the responsiveness of the 6.3 kbmore »
- Authors:
-
- Univ. of Adelaide, SA (Australia)
- Univ. of South Australia, Adelaide, SA (Australia)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Physical and Life Sciences Directorate; Univ. of California, Merced, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); National Health and Medical Research Council of Australia (NHMRC); National Institutes of Health (NIH)
- OSTI Identifier:
- 1891707
- Alternate Identifier(s):
- OSTI ID: 1249889
- Report Number(s):
- LLNL-JRNL-837178
Journal ID: ISSN 0303-7207; 1056922
- Grant/Contract Number:
- AC52-07NA27344; ID565372; DK075730
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Molecular and Cellular Endocrinology
- Additional Journal Information:
- Journal Volume: 413; Journal ID: ISSN 0303-7207
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biological sciences; medical sciences; vitamin D; vitamin D response element; SOST; sclerostin; osteocyte; gene regulation; promoter
Citation Formats
Wijenayaka, Asiri R., Yang, Dongqing, Prideaux, Matthew, Ito, Nobuaki, Kogawa, Masakazu, Anderson, Paul H., Morris, Howard A., Solomon, Lucian B., Loots, Gabriela G., Findlay, David M., and Atkins, Gerald J. 1α,25-dihydroxyvitamin D3 stimulates human SOST gene expression and sclerostin secretion. United States: N. p., 2015.
Web. doi:10.1016/j.mce.2015.06.021.
Wijenayaka, Asiri R., Yang, Dongqing, Prideaux, Matthew, Ito, Nobuaki, Kogawa, Masakazu, Anderson, Paul H., Morris, Howard A., Solomon, Lucian B., Loots, Gabriela G., Findlay, David M., & Atkins, Gerald J. 1α,25-dihydroxyvitamin D3 stimulates human SOST gene expression and sclerostin secretion. United States. https://doi.org/10.1016/j.mce.2015.06.021
Wijenayaka, Asiri R., Yang, Dongqing, Prideaux, Matthew, Ito, Nobuaki, Kogawa, Masakazu, Anderson, Paul H., Morris, Howard A., Solomon, Lucian B., Loots, Gabriela G., Findlay, David M., and Atkins, Gerald J. Tue .
"1α,25-dihydroxyvitamin D3 stimulates human SOST gene expression and sclerostin secretion". United States. https://doi.org/10.1016/j.mce.2015.06.021. https://www.osti.gov/servlets/purl/1891707.
@article{osti_1891707,
title = {1α,25-dihydroxyvitamin D3 stimulates human SOST gene expression and sclerostin secretion},
author = {Wijenayaka, Asiri R. and Yang, Dongqing and Prideaux, Matthew and Ito, Nobuaki and Kogawa, Masakazu and Anderson, Paul H. and Morris, Howard A. and Solomon, Lucian B. and Loots, Gabriela G. and Findlay, David M. and Atkins, Gerald J.},
abstractNote = {Sclerostin, the SOST gene product, is a negative regulator of bone formation and a positive regulator of bone resorption. Here, in this study, treatment of human primary osteoblasts, including cells differentiated to an osteocyte-like stage, with 1α,25-dihydroxyvitaminD3 (1,25D) resulted in the dose-dependent increased expression of SOST mRNA. A similar effect was observed in human trabecular bone samples cultured ex vivo, and in osteocyte-like cultures of differentiated SAOS2 cells. Treatment of SAOS2 cells with 1,25D resulted in the production and secretion of sclerostin protein. In silico analysis of the human SOST gene revealed a single putative DR3-type vitamin D response element (VDRE) at position -6216 bp upstream of the transcription start site (TSS). This sequence was confirmed to have strong VDRE activity by luciferase reporter assays and electrophoretic mobility shift analysis (EMSA). Sequence substitution in the VDR/RXR half-sites abolished VDRE reporter activity and binding of nuclear proteins. A 6.3 kb fragment of the human proximal SOST promoter demonstrated responsiveness to 1,25D. The addition of the evolutionary conserved region 5 (ECR5), a known bone specific enhancer region, ahead of the 6.3 kb fragment increased basal promoter activity but did not increase 1,25D responsiveness. Site-specific mutagenesis abolished the responsiveness of the 6.3 kb promoter to 1,25D. We conclude that 1,25D is a direct regulator of human SOST gene and sclerostin protein expression, extending the pathways of control of sclerostin expression. At least some of this responsiveness is mediated by the identified classical VDRE however the nature of the transcriptional regulation by 1,25D warrants further investigation.},
doi = {10.1016/j.mce.2015.06.021},
journal = {Molecular and Cellular Endocrinology},
number = ,
volume = 413,
place = {United States},
year = {Tue Jun 23 00:00:00 EDT 2015},
month = {Tue Jun 23 00:00:00 EDT 2015}
}
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Works referenced in this record:
Changing RANKL/OPG mRNA expression in differentiating murine primary osteoblasts
journal, August 2001
- Thomas, Gp; Baker, Su; Eisman, Ja
- Journal of Endocrinology, Vol. 170, Issue 2
Kidney Function and Influence of Sunlight Exposure in Patients With Impaired 24-Hydroxylation of Vitamin D Due to CYP24A1 Mutations
journal, January 2015
- Figueres, Marie-Lucile; Linglart, Agnès; Bienaime, Frank
- American Journal of Kidney Diseases, Vol. 65, Issue 1
RANKL Expression Is Related to the Differentiation State of Human Osteoblasts
journal, June 2003
- Atkins, Gerald J.; Kostakis, Panagiota; Pan, Beiqing
- Journal of Bone and Mineral Research, Vol. 18, Issue 6
Targeted Deletion of the Sclerostin Gene in Mice Results in Increased Bone Formation and Bone Strength
journal, February 2008
- Li, Xiaodong; Ominsky, Michael S.; Niu, Qing-Tian
- Journal of Bone and Mineral Research, Vol. 23, Issue 6
Pro-Inflammatory Cytokines TNF-Related Weak Inducer of Apoptosis (TWEAK) and TNFα Induce the Mitogen-Activated Protein Kinase (MAPK)-Dependent Expression of Sclerostin in Human Osteoblasts*
journal, August 2009
- Vincent, Cristina; Findlay, David M.; Welldon, Katie J.
- Journal of Bone and Mineral Research, Vol. 24, Issue 8
Nuclear Receptor Coactivators Facilitate Vitamin D Receptor Homodimer Action on Direct Repeat Hormone Response Elements
journal, March 2000
- Takeshita, Akira; Ozawa, Yasunori; Chin, William W.
- Endocrinology, Vol. 141, Issue 3
Autocrine/Paracrine Action of Vitamin D on FGF23 Expression in Cultured Rat Osteoblasts
journal, March 2010
- Tang, Wen-Jing; Wang, Li-Fang; Xu, Xiao-Ya
- Calcified Tissue International, Vol. 86, Issue 5
Activation of Receptor Activator of NF-κB Ligand Gene Expression by 1,25-Dihydroxyvitamin D
3
Is Mediated through Multiple Long-Range Enhancers
journal, September 2006
- Kim, Sungtae; Yamazaki, Miwa; Zella, Lee A.
- Molecular and Cellular Biology, Vol. 26, Issue 17
Sclerostin is a locally acting regulator of late-osteoblast/preosteocyte differentiation and regulates mineralization through a MEPE-ASARM-dependent mechanism
journal, June 2011
- Atkins, Gerald J.; Rowe, Peter S.; Lim, Hui P.
- Journal of Bone and Mineral Research, Vol. 26, Issue 7
Oncostatin M promotes bone formation independently of resorption when signaling through leukemia inhibitory factor receptor in mice
journal, February 2010
- Walker, Emma C.; McGregor, Narelle E.; Poulton, Ingrid J.
- Journal of Clinical Investigation, Vol. 120, Issue 2
Long term oncostatin M treatment induces an osteocyte-like differentiation on osteosarcoma and calvaria cells
journal, May 2009
- Brounais, Bénédicte; David, Emmanuelle; Chipoy, Céline
- Bone, Vol. 44, Issue 5
Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: An in vitro model of osteoblast development
journal, June 1992
- Quarles, L. Darryl; Yohay, Daniel A.; Lever, Laura W.
- Journal of Bone and Mineral Research, Vol. 7, Issue 6
The Osteoblast to Osteocyte Transition: Epigenetic Changes and Response to the Vitamin D 3 Hormone
journal, July 2014
- St. John, Hillary C.; Bishop, Kathleen A.; Meyer, Mark B.
- Molecular Endocrinology, Vol. 28, Issue 7
Effects of intermittent parathyroid hormone (PTH) administration on SOST mRNA and protein in rat bone
journal, June 2007
- Silvestrini, G.; Ballanti, P.; Leopizzi, M.
- Journal of Molecular Histology, Vol. 38, Issue 4
Vitamin K promotes mineralization, osteoblast-to-osteocyte transition, and an anticatabolic phenotype by γ-carboxylation-dependent and -independent mechanisms
journal, December 2009
- Atkins, Gerald J.; Welldon, Katie J.; Wijenayaka, Asiri R.
- American Journal of Physiology-Cell Physiology, Vol. 297, Issue 6
SOST is a target gene for PTH in bone
journal, August 2005
- Keller, Hansjoerg; Kneissel, Michaela
- Bone, Vol. 37, Issue 2
Vitamin D and bone
journal, October 2002
- Suda, Tatsuo; Ueno, Yutaka; Fujii, Katsuyuki
- Journal of Cellular Biochemistry, Vol. 88, Issue 2
Six Genes Expressed in Bones and Teeth Encode the Current Members of the SIBLING Family of Proteins
journal, January 2003
- Fisher, Larry W.; Fedarko, Neal S.
- Connective Tissue Research, Vol. 44, Issue 1
Metabolism of vitamin D3 in human osteoblasts: Evidence for autocrine and paracrine activities of 1α,25-dihydroxyvitamin D3
journal, June 2007
- Atkins, Gerald J.; Anderson, Paul H.; Findlay, David M.
- Bone, Vol. 40, Issue 6
1α,25-Dihydroxyvitamin D3upregulates FGF23 gene expression in bone: the final link in a renal-gastrointestinal-skeletal axis that controls phosphate transport
journal, December 2005
- Kolek, Olga I.; Hines, Eric R.; Jones, Marci D.
- American Journal of Physiology-Gastrointestinal and Liver Physiology, Vol. 289, Issue 6
Parathyroid hormone (PTH)–induced bone gain is blunted in SOST overexpressing and deficient mice
journal, December 2009
- Kramer, Ina; Loots, Gabriela G.; Studer, Anne
- Journal of Bone and Mineral Research, Vol. 25, Issue 2
Sclerostin Mediates Bone Response to Mechanical Unloading Through Antagonizing Wnt/β-Catenin Signaling
journal, October 2009
- Lin, Chuwen; Jiang, Xuan; Dai, Zhongquan
- Journal of Bone and Mineral Research, Vol. 24, Issue 10
Stimulation by 1,25-Dihydroxyvitamin D3 of in vitro mineralization induced by osteoblast-like MC3T3-E1 cells
journal, January 1991
- Matsumoto, T.; Igarashi, C.; Takeuchi, Y.
- Bone, Vol. 12, Issue 1
Sclerostin is a delayed secreted product of osteocytes that inhibits bone formation
journal, November 2005
- Poole, Kenneth E. S.; van Bezooijen, Rutger L.; Loveridge, Nigel
- The FASEB Journal, Vol. 19, Issue 13
Transcriptional activation through the vitamin D receptor in osteoblasts
journal, January 1998
- MacDonald, Paul N.
- Frontiers in Bioscience, Vol. 3, Issue 4
Mechanical Stimulation of Bone in Vivo Reduces Osteocyte Expression of Sost/Sclerostin
journal, December 2007
- Robling, Alexander G.; Niziolek, Paul J.; Baldridge, Lee A.
- Journal of Biological Chemistry, Vol. 283, Issue 9
Identification of a 52 kb deletion downstream of the SOST gene in patients with van Buchem disease
journal, February 2002
- Balemans, W.
- Journal of Medical Genetics, Vol. 39, Issue 2
Importance of 1,25-dihydroxyvitamin D3 and the nonadherent cells of marrow for osteoblast differentiation from rat marrow stromal cells
journal, June 1995
- Rickard, D. J.; Kazhdan, I.; Leboy, P. S.
- Bone, Vol. 16, Issue 6
Developments in Sclerostin Biology: Regulation of Gene Expression, Mechanisms of Action, and Physiological Functions
journal, January 2014
- Weivoda, Megan M.; Oursler, Merry Jo
- Current Osteoporosis Reports, Vol. 12, Issue 1
Electrophoretic mobility shift assay for the detection of specific DNA–protein complex in nuclear extracts from the cultured cells and frozen autopsy human brain tissue
journal, July 2000
- Lahiri, Debomoy K.; Ge, Yuan-Wen
- Brain Research Protocols, Vol. 5, Issue 3
The induction of a catabolic phenotype in human primary osteoblasts and osteocytes by polyethylene particles
journal, August 2009
- Atkins, Gerald J.; Welldon, Katie J.; Holding, Christopher A.
- Biomaterials, Vol. 30, Issue 22
Molecular analysis of DMP1 mutants causing autosomal recessive hypophosphatemic rickets
journal, February 2009
- Farrow, Emily G.; Davis, Siobhan I.; Ward, Leanne M.
- Bone, Vol. 44, Issue 2
Identification of a DNA sequence responsible for binding of the 1,25-dihydroxyvitamin D3 receptor and 1,25-dihydroxyvitamin D3 enhancement of mouse secreted phosphoprotein 1 (SPP-1 or osteopontin) gene expression.
journal, December 1990
- Noda, M.; Vogel, R. L.; Craig, A. M.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 24
Serum Sclerostin Increases in Healthy Adult Men during Bed Rest
journal, September 2012
- Spatz, J. M.; Fields, E. E.; Yu, E. W.
- The Journal of Clinical Endocrinology & Metabolism, Vol. 97, Issue 9
Chronic Elevation of Parathyroid Hormone in Mice Reduces Expression of Sclerostin by Osteocytes: A Novel Mechanism for Hormonal Control of Osteoblastogenesis
journal, November 2005
- Bellido, T.; Ali, A. A.; Gubrij, I.
- Endocrinology, Vol. 146, Issue 11
Sclerostin Stimulates Osteocyte Support of Osteoclast Activity by a RANKL-Dependent Pathway
journal, October 2011
- Wijenayaka, Asiri R.; Kogawa, Masakazu; Lim, Hui Peng
- PLoS ONE, Vol. 6, Issue 10
Osteocyte control of osteoclastogenesis
journal, June 2013
- O'Brien, Charles A.; Nakashima, Tomoki; Takayanagi, Hiroshi
- Bone, Vol. 54, Issue 2
Genomic deletion of a long-range bone enhancer misregulates sclerostin in Van Buchem disease
journal, June 2005
- Loots, G. G.
- Genome Research, Vol. 15, Issue 7
Large-Scale in Silico and Microarray-Based Identification of Direct 1,25-Dihydroxyvitamin D3 Target Genes
journal, November 2005
- Wang, Tian-Tian; Tavera-Mendoza, Luz Elisa; Laperriere, David
- Molecular Endocrinology, Vol. 19, Issue 11
Regulation of Bone Sialoprotein and Osteopontin mRNA Expression by Dexamethasone and 1,25-dihydroxyvitamin D3in Rat Bone Organ Cultures
journal, January 1996
- Chen, Jinkun; Thomas, Huw F.; Sodek, Jaro
- Connective Tissue Research, Vol. 34, Issue 1
Sclerostin Regulates Release of Bone Mineral by Osteocytes by Induction of Carbonic Anhydrase 2: SCLEROSTIN INDUCES OSTEOCYTIC OSTEOLYSIS
journal, November 2013
- Kogawa, Masakazu; Wijenayaka, Asiri R.; Ormsby, Renee T.
- Journal of Bone and Mineral Research, Vol. 28, Issue 12
Osteocyte-Derived Sclerostin Inhibits Bone Formation: Its Role in Bone Morphogenetic Protein and Wnt Signaling
journal, January 2008
- ten Dijke, Peter; Krause, Carola; de Gorter, David J. J.
- Journal of Bone and Joint Surgery, Vol. 90, Issue Supplement_1
TWEAK and TNF Regulation of Sclerostin: A Novel Pathway for the Regulation of Bone Remodelling
book, October 2010
- Findlay, David M.; Atkins, Gerald J.
- Advances in Experimental Medicine and Biology
Single-dose, placebo-controlled, randomized study of AMG 785, a sclerostin monoclonal antibody
journal, June 2010
- Padhi, Desmond; Jang, Graham; Stouch, Brian
- Journal of Bone and Mineral Research, Vol. 26, Issue 1
Wnt Signaling: A Key Regulator of Bone Mass
book, January 2006
- Baron, Roland; Rawadi, Georges; Roman‐Roman, Sergio
- Current Topics in Developmental Biology
Expression of Fibroblast Growth Factor 23, Vitamin D Receptor, and Sclerostin in Bone Tissue from Hypercalciuric Stone Formers
journal, April 2014
- Menon, Viviane Barcellos; Moysés, Rosa Maria Affonso; Gomes, Samirah Abreu
- Clinical Journal of the American Society of Nephrology, Vol. 9, Issue 7
A 52-kb deletion in theSOST-MEOX1 intergenic region on 17q12-q21 is associated with van Buchem disease in the Dutch population
journal, May 2002
- Staehling-Hampton, Karen; Proll, Sean; Paeper, Bryan W.
- American Journal of Medical Genetics, Vol. 110, Issue 2
The amazing osteocyte
journal, January 2011
- Bonewald, Lynda F.
- Journal of Bone and Mineral Research, Vol. 26, Issue 2
HDAC5 Controls MEF2C-Driven Sclerostin Expression in Osteocytes: HDAC5 REGULATES SCLEROSTIN LEVELS IN OSTEOCYTES
journal, February 2015
- Wein, Marc N.; Spatz, Jordan; Nishimori, Shigeki
- Journal of Bone and Mineral Research, Vol. 30, Issue 3
Osteocyte regulation of bone mineral: a little give and take
journal, February 2012
- Atkins, G. J.; Findlay, D. M.
- Osteoporosis International, Vol. 23, Issue 8
Repression of basal transcription by vitamin D receptor: evidence for interaction of unliganded vitamin D receptor with two receptor interaction domains in RIP13delta1
journal, June 1998
- Dwivedi, Pp; Muscat, Ge; Bailey, Pj
- Journal of Molecular Endocrinology, Vol. 20, Issue 3
SaOS2 Osteosarcoma Cells as an In Vitro Model for Studying the Transition of Human Osteoblasts to Osteocytes
journal, June 2014
- Prideaux, Matthew; Wijenayaka, Asiri R.; Kumarasinghe, Duminda D.
- Calcified Tissue International, Vol. 95, Issue 2
Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism
journal, October 2006
- Feng, Jian Q.; Ward, Leanne M.; Liu, Shiguang
- Nature Genetics, Vol. 38, Issue 11
Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1
journal, July 1991
- Simmons, Pj; Torok-Storb, B.
- Blood, Vol. 78, Issue 1
Unlocking thesostgene
journal, February 2015
- Johnson, Mark L.
- Journal of Bone and Mineral Research, Vol. 30, Issue 3
Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease
journal, May 2013
- Teramachi, Jumpei; Hiruma, Yuko; Ishizuka, Seiichi
- Journal of Bone and Mineral Research, Vol. 28, Issue 6
Control of the SOST Bone Enhancer by PTH Using MEF2 Transcription Factors
journal, August 2007
- Leupin, Olivier; Kramer, Ina; Collette, Nicole M.
- Journal of Bone and Mineral Research, Vol. 22, Issue 12
Further Characterization of Cells Expressing STRO-1 in Cultures of Adult Human Bone Marrow Stromal Cells
journal, August 1999
- Stewart, Karina; Walsh, Susan; Screen, Joanne
- Journal of Bone and Mineral Research, Vol. 14, Issue 8
Extracellular phosphate modulates the effect of 1α,25-dihydroxy vitamin D3 (1,25D) on osteocyte like cells
journal, July 2013
- Ito, Nobuaki; Findlay, David M.; Anderson, Paul H.
- The Journal of Steroid Biochemistry and Molecular Biology, Vol. 136
Works referencing / citing this record:
Hormonal Regulation of Osteocyte Perilacunar and Canalicular Remodeling in the Hyp Mouse Model of X-Linked Hypophosphatemia: OSTEOCYTE PERILACUAR AND CANALICULAR REMODELING IN HYP MICE
journal, November 2017
- Tokarz, Danielle; Martins, Janaina S.; Petit, Elizabeth T.
- Journal of Bone and Mineral Research, Vol. 33, Issue 3
Vitamin D–regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification
journal, July 2019
- Nguyen-Yamamoto, Loan; Tanaka, Ken-Ichiro; St–Arnaud, Rene
- JCI Insight, Vol. 4, Issue 13
Sclerostin, an emerging therapeutic target for treating osteoporosis and osteoporotic fracture: A general review
journal, January 2016
- Suen, Pui Kit; Qin, Ling
- Journal of Orthopaedic Translation, Vol. 4
DObesity: Relationship between vitamin D deficiency, obesity and sclerostin as a novel biomarker of bone metabolism
journal, September 2019
- Azzam, Eman Zaki; Ata, Mohamed Nabil; Younan, Doreen Nazeih
- Journal of Clinical & Translational Endocrinology, Vol. 17
Sclerostin and chronic kidney disease
journal, December 2017
- Mierzwińska, Emilia; Hryszko, Tomasz; Szablak-Uliszewska, Emilia
- Postępy Higieny i Medycyny Doświadczalnej, Vol. 71, Issue 0
Control of Bone Homeostasis by the Wnt Inhibitor Sclerostin
journal, June 2016
- McGee-Lawrence, Meghan E.; Hamrick, Mark W.
- Current Molecular Biology Reports, Vol. 2, Issue 3
Relationship between plasma levels of sclerostin, calcium–phosphate disturbances, established markers of bone turnover, and inflammation in haemodialysis patients
journal, December 2018
- Pietrzyk, Barbara; Wyskida, Katarzyna; Ficek, Joanna
- International Urology and Nephrology, Vol. 51, Issue 3
Role of the Wnt/β-Catenin Pathway in Renal Osteodystrophy
journal, January 2018
- Bisson, Sarah-Kim; Ung, Roth-Visal; Mac-Way, Fabrice
- International Journal of Endocrinology, Vol. 2018
Role of the Wnt/β-Catenin Pathway in Renal Osteodystrophy
journal, January 2018
- Bisson, Sarah-Kim; Ung, Roth-Visal; Mac-Way, Fabrice
- International Journal of Endocrinology, Vol. 2018
1,25-Dihydroxyvitamin D Alone Improves Skeletal Growth, Microarchitecture, and Strength in a Murine Model of XLH, Despite Enhanced FGF23 Expression
journal, February 2016
- Liu, Eva S.; Martins, Janaina S.; Raimann, Adalbert
- Journal of Bone and Mineral Research, Vol. 31, Issue 5