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Title: Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens

Abstract

In contrast to homeohydric vascular plants, mosses employ a poikilohydric strategy for surviving in the dry aerial environment. A detailed understanding of the structure, composition, and development of moss cell walls can contribute to our understanding of not only the evolution of overall cell wall complexity, but also the differences that have evolved in response to selection for different survival strategies. The model moss species Physcomitrella patens has a predominantly haploid lifecycle consisting of protonemal filaments that regenerate from protoplasts and enlarge by tip growth, and leafy gametophores composed of cells that enlarge by diffuse growth and differentiate into several different types. Advantages for genetic studies include methods for efficient targeted gene modification and extensive genomic resources. Immuno and affinity cytochemical labeling were used to examine the distribution of polysaccharides and proteins in regenerated protoplasts, protonemal filaments, rhizoids, and sectioned gametophores of P. patens. The cell wall composition of regenerated protoplasts was also characterized by flow cytometry. Crystalline cellulose was abundant in the cell walls of regenerating protoplasts and protonemal cells that developed on media of high osmolarity, whereas homogalactuonan was detected in the walls of protonemal cells that developed on low osmolarity media and not in regenerating protoplasts. Mannanmore » was the major hemicellulose detected in all tissues tested. Arabinogalactan proteins were detected in different cell types by different probes, consistent with structural heterogneity. The results reveal developmental and cell type specific differences in cell wall composition and provide a basis for analyzing cell wall phenotypes in knockout mutants.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of Rhode Island, Kingston, RI (United States). Dept. of Biological Sciences
Publication Date:
Research Org.:
Univ. of Rhode Island, Kingston, RI (United States). Dept. of Biological Sciences; Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1249327
Grant/Contract Number:  
SC0001090; IOS-1257047
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; affinity cytochemistry; bryophyte; cell wall, flow cytometry; immunofluorescence; Physcomitrella patens

Citation Formats

Berry, Elizabeth A., Tran, Mai L., Dimos, Christos S., Budziszek, Michael J., Scavuzzo-Duggan, Tess R., and Roberts, Alison W. Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens. United States: N. p., 2016. Web. doi:10.3389/fpls.2016.00248.
Berry, Elizabeth A., Tran, Mai L., Dimos, Christos S., Budziszek, Michael J., Scavuzzo-Duggan, Tess R., & Roberts, Alison W. Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens. United States. doi:10.3389/fpls.2016.00248.
Berry, Elizabeth A., Tran, Mai L., Dimos, Christos S., Budziszek, Michael J., Scavuzzo-Duggan, Tess R., and Roberts, Alison W. Tue . "Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens". United States. doi:10.3389/fpls.2016.00248. https://www.osti.gov/servlets/purl/1249327.
@article{osti_1249327,
title = {Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens},
author = {Berry, Elizabeth A. and Tran, Mai L. and Dimos, Christos S. and Budziszek, Michael J. and Scavuzzo-Duggan, Tess R. and Roberts, Alison W.},
abstractNote = {In contrast to homeohydric vascular plants, mosses employ a poikilohydric strategy for surviving in the dry aerial environment. A detailed understanding of the structure, composition, and development of moss cell walls can contribute to our understanding of not only the evolution of overall cell wall complexity, but also the differences that have evolved in response to selection for different survival strategies. The model moss species Physcomitrella patens has a predominantly haploid lifecycle consisting of protonemal filaments that regenerate from protoplasts and enlarge by tip growth, and leafy gametophores composed of cells that enlarge by diffuse growth and differentiate into several different types. Advantages for genetic studies include methods for efficient targeted gene modification and extensive genomic resources. Immuno and affinity cytochemical labeling were used to examine the distribution of polysaccharides and proteins in regenerated protoplasts, protonemal filaments, rhizoids, and sectioned gametophores of P. patens. The cell wall composition of regenerated protoplasts was also characterized by flow cytometry. Crystalline cellulose was abundant in the cell walls of regenerating protoplasts and protonemal cells that developed on media of high osmolarity, whereas homogalactuonan was detected in the walls of protonemal cells that developed on low osmolarity media and not in regenerating protoplasts. Mannan was the major hemicellulose detected in all tissues tested. Arabinogalactan proteins were detected in different cell types by different probes, consistent with structural heterogneity. The results reveal developmental and cell type specific differences in cell wall composition and provide a basis for analyzing cell wall phenotypes in knockout mutants.},
doi = {10.3389/fpls.2016.00248},
journal = {Frontiers in Plant Science},
number = ,
volume = 7,
place = {United States},
year = {2016},
month = {3}
}

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