Energy optimization of a regular macromolecular crystallography beamline for ultra-high-resolution crystallography

Journal Article · 31 December 2014 · Journal of Synchrotron Radiation (Online)

DOI: https://doi.org/10.1107/S1600577514022619 · OSTI ID:1248637

Rosenbaum, Gerd ^[1]; Ginell, Stephan L. ^[2]; Chen, Julian C.-H. ^[3]

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1. Argonne National Lab. (ANL), Argonne, IL (United States); The Univ. of Georgia, Athens, GA (United States)
2. Argonne National Lab. (ANL), Argonne, IL (United States)
3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); The Univ. of Toledo, Toledo, OH (United States)

In this study, a practical method for operating existing undulator synchrotron beamlines at photon energies considerably higher than their standard operating range is described and applied at beamline 19-ID of the Structural Biology Center at the Advanced Photon Source enabling operation at 30 keV. Adjustments to the undulator spectrum were critical to enhance the 30 keV flux while reducing the lower- and higher-energy harmonic contamination. A Pd-coated mirror and Al attenuators acted as effective low- and high-bandpass filters. The resulting flux at 30 keV, although significantly lower than with X-ray optics designed and optimized for this energy, allowed for accurate data collection on crystals of the small protein crambin to 0.38 Å resolution.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE
• Grant/Contract Number: AC52-06NA25396
• OSTI ID: 1248637
• Report Number(s): LA-UR--15-20476; PII: S1600577514022619
• Journal Information: Journal of Synchrotron Radiation (Online), Journal Name: Journal of Synchrotron Radiation (Online) Journal Issue: 1 Vol. 22; ISSN 1600-5775; ISSN JSYRES
• Publisher: International Union of CrystallographyCopyright Statement
• Country of Publication: United States
• Language: English