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Title: Structure and mechanism of the phage T4 recombination mediator protein UvsY

The UvsY recombination mediator protein is critical for efficient homologous recombination in bacteriophage T4 and is the functional analog of the eukaryotic Rad52 protein. During T4 homologous recombination, the UvsX recombinase has to compete with the prebound gp32 single-stranded binding protein for DNA-binding sites and UvsY stimulates this filament nucleation event. We report here the crystal structure of UvsY in four similar open-barrel heptameric assemblies and provide structural and biophysical insights into its function. The UvsY heptamer was confirmed in solution by centrifugation and light scattering, and thermodynamic analyses revealed that the UvsY–ssDNA interaction occurs within the assembly via two distinct binding modes. Using surface plasmon resonance, we also examined the binding of UvsY to both ssDNA and the ssDNA–gp32 complex. These analyses confirmed that ssDNA can bind UvsY and gp32 independently and also as a ternary complex. They also showed that residues located on the rim of the heptamer are required for optimal binding to ssDNA, thus identifying the putative ssDNA-binding surface. We propose a model in which UvsY promotes a helical ssDNA conformation that disfavors the binding of gp32 and initiates the assembly of the ssDNA–UvsX filament.
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [2] ;  [3]
  1. St. Jude Children's Research Hospital, Memphis, TN (United States). Dept. of Structural Biology
  2. Vanderbilt Univ., Nashville, TN (United States). Dept. of Chemistry, Pharmacology, and Biomedical Informatics
  3. St. Jude Children's Research Hospital, Memphis, TN (United States). Dept. of Structural Biology; Univ. of Tennessee Health Science Center, Memphis, TN (United States). Dept. of Microbiology, Immunology and Biochemistry
Publication Date:
Grant/Contract Number:
W‐31‐109‐Eng‐38; GM066934; CA21765; GM080403; GM099842; DK097376; HL122010; GM073151; AI117905; CHE 1305874
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 113; Journal Issue: 12; Journal ID: ISSN 0027-8424
National Academy of Sciences, Washington, DC (United States)
Research Org:
St. Jude Children's Research Hospital, Memphis, TN (United States)
Sponsoring Org:
USDOE; National Inst. of Health (NIH) (United States); American Lebanese Syrian Associated Charities (ALSAC) (United States); National Science Foundation (NSF)
Contributing Orgs:
Univ. of Tennessee Health Science Center, Memphis, TN (United States); Vanderbilt Univ., Nashville, TN (United States)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; homologous recombination; structural modification; DNA binding; DNA architecture; crystallography
OSTI Identifier: