skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution

Abstract

Here, we developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.

Authors:
 [1];  [1];  [1];  [1];  [2];  [2];  [3];  [3];  [1];  [1];  [1];  [1];  [4];  [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Diamond Light Source Ltd., Oxfordshire (United Kingdom)
  3. Univ. College London, London (United Kingdom); Rutherford Appleton Lab., Oxfordshire (United Kingdom)
  4. Brookhaven National Lab. (BNL), Upton, NY (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1248377
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; fluorescence imaging; imaging techniques

Citation Formats

Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, and Chu, Yong S.. Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution. United States: N. p., 2016. Web. https://doi.org/10.1038/srep20112.
Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, & Chu, Yong S.. Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution. United States. https://doi.org/10.1038/srep20112
Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, and Chu, Yong S.. Fri . "Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution". United States. https://doi.org/10.1038/srep20112. https://www.osti.gov/servlets/purl/1248377.
@article{osti_1248377,
title = {Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution},
author = {Yan, Hanfei and Nazaretski, Evgeny and Lauer, Kenneth R. and Huang, Xiaojing and Wagner, Ulrich and Rau, Christoph and Yusuf, Mohammed and Robinson, Ian K. and Kalbfleisch, Sebastian and Li, Li and Bouet, Nathalie and Zhou, Juan and Conley, Ray and Chu, Yong S.},
abstractNote = {Here, we developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.},
doi = {10.1038/srep20112},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {2016},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 15 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Quantitative x-ray phase imaging at the nanoscale by multilayer Laue lenses
journal, February 2013

  • Yan, Hanfei; Chu, Yong S.; Maser, Jörg
  • Scientific Reports, Vol. 3, Issue 1
  • DOI: 10.1038/srep01307

Measurements of wet metaphase chromosomes in the scanning transmission X-ray microscope
journal, May 1993


Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction
journal, January 2009


Takagi-Taupin description of x-ray dynamical diffraction from diffractive optics with large numerical aperture
journal, September 2007


Transmission X-ray microscopy for full-field nano imaging of biomaterials
journal, August 2010

  • Andrews, Joy C.; Meirer, Florian; Liu, Yijin
  • Microscopy Research and Technique, Vol. 74, Issue 7
  • DOI: 10.1002/jemt.20907

Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods
journal, February 2015


Platinum blue as an alternative to uranyl acetate for staining in transmission electron microscopy
journal, January 2007

  • Inaga, Sumire; Katsumoto, Tetsuo; Tanaka, Keiichi
  • Archives of Histology and Cytology, Vol. 70, Issue 1
  • DOI: 10.1679/aohc.70.43

Three-dimensional reconstruction of a human metaphase chromosome from electron micrographs
journal, September 1987

  • Harauz, George; Borland, Lisa; Bahr, Gunter F.
  • Chromosoma, Vol. 95, Issue 5
  • DOI: 10.1007/bf00293184

Optimization of multilayer Laue lenses for a scanning X-ray microscope
journal, November 2012


Pushing the limits: an instrument for hard X-ray imaging below 20 nm
journal, January 2015


Karyotyping of Single Human Chromosomes from Dry Mass Determined by Electron Microscopy
journal, April 1971

  • Bahr, G. F.; Golomb, H. M.
  • Proceedings of the National Academy of Sciences, Vol. 68, Issue 4
  • DOI: 10.1073/pnas.68.4.726

Recent advances in synchrotron-based hard x-ray phase contrast imaging
journal, November 2013


Platinblau [blue platinum amide complex]
journal, May 1969

  • Brown, David Basset; Burbank, R. D.; Robin, M. B.
  • Journal of the American Chemical Society, Vol. 91, Issue 11
  • DOI: 10.1021/ja01039a012

Imaging of DNA in human and plant chromosomes by high-resolution scanning electron microscopy
journal, September 1995


Oxidation of PtNi nanoparticles studied by a scanning X-ray fluorescence microscope with multi-layer Laue lenses
journal, January 2013

  • Kang, Hyon Chol; Yan, Hanfei; Chu, Yong S.
  • Nanoscale, Vol. 5, Issue 16
  • DOI: 10.1039/c3nr00396e

Condensed Mitotic Chromosome Structure at Nanometer Resolution Using PALM and EGFP- Histones
journal, September 2010


Chemical composition mapping with nanometre resolution by soft X-ray microscopy
journal, September 2014


Focused ion beam (FIB) combined with high resolution scanning electron microscopy: A promising tool for 3D analysis of chromosome architecture
journal, February 2009

  • Schroeder-Reiter, Elizabeth; Pérez-Willard, Fabián; Zeile, Ulrike
  • Journal of Structural Biology, Vol. 165, Issue 2
  • DOI: 10.1016/j.jsb.2008.10.002

The nature and mechanisms of chromosome banding
journal, May 1982


Mapping biological composition through quantitative phase and absorption X-ray ptychography
journal, October 2014

  • Jones, Michael W. M.; Elgass, Kirstin; Junker, Mark D.
  • Scientific Reports, Vol. 4, Issue 1
  • DOI: 10.1038/srep06796

Mass Density and Water Content of Saturated Never-Dried Calcium Silicate Hydrates
journal, March 2015

  • da Silva, Julio C.; Trtik, Pavel; Diaz, Ana
  • Langmuir, Vol. 31, Issue 13
  • DOI: 10.1021/la504478j

Nanoscale Imaging of Mineral Crystals inside Biological Composite Materials Using X-Ray Diffraction Microscopy
journal, January 2008


Hard x-ray nanofocusing by multilayer Laue lenses
journal, June 2014


The Race to X-ray Microbeam and Nanobeam Science
journal, December 2011


11 nm hard X-ray focus from a large-aperture multilayer Laue lens
journal, December 2013

  • Huang, Xiaojing; Yan, Hanfei; Nazaretski, Evgeny
  • Scientific Reports, Vol. 3, Issue 1
  • DOI: 10.1038/srep03562

Hard X-ray nanoprobe investigations of the subtissue metal distributions within Daphnia magna
journal, May 2013

  • De Samber, B.; De Schamphelaere, K. A. C.; Janssen, C. R.
  • Analytical and Bioanalytical Chemistry, Vol. 405, Issue 18
  • DOI: 10.1007/s00216-013-7019-6

Scanning electron microscope studies of human metaphase chromosomes
journal, March 2014

  • Shemilt, L. A.; Estandarte, A. K. C.; Yusuf, M.
  • Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 372, Issue 2010
  • DOI: 10.1098/rsta.2013.0144

The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities
journal, December 2013


Three-dimensional reconstruction of a human metaphase chromosome from electron micrographs
journal, September 1987

  • Harauz, George; Borland, Lisa; Bahr, Gunter F.
  • Chromosoma, Vol. 95, Issue 5
  • DOI: 10.1007/BF00293184

Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction
journal, January 2009


The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities
journal, December 2013


Nanoscale Imaging of Mineral Crystals inside Biological Composite Materials Using X-Ray Diffraction Microscopy
journal, January 2008


Pushing the limits: an instrument for hard X-ray imaging below 20 nm
journal, January 2015


Takagi-Taupin description of x-ray dynamical diffraction from diffractive optics with large numerical aperture
journal, September 2007


Optimization of multilayer Laue lenses for a scanning X-ray microscope
journal, November 2012


Imaging of DNA in human and plant chromosomes by high-resolution scanning electron microscopy
journal, September 1995


The nature and mechanisms of chromosome banding
journal, May 1982


    Works referencing / citing this record:

    Electrochemical (de)lithiation of silver ferrite and composites: mechanistic insights from ex situ, in situ, and operando X-ray techniques
    journal, January 2017

    • Durham, Jessica L.; Brady, Alexander B.; Cama, Christina A.
    • Physical Chemistry Chemical Physics, Vol. 19, Issue 33
    • DOI: 10.1039/c7cp04012a

    Microscopy Instrumentation and Nanopositioning at NSLS-II: Current Status and Future Directions
    journal, September 2018


    Imaging nanoscale lattice variations by machine learning of x-ray diffraction microscopy data
    journal, August 2016


    Unambiguous Intracellular Localization and Quantification of a Potent Iridium Anticancer Compound by Correlative 3D Cryo X‐Ray Imaging
    journal, December 2019

    • Conesa, José Javier; Carrasco, Ana C.; Rodríguez‐Fanjul, Vanessa
    • Angewandte Chemie, Vol. 132, Issue 3
    • DOI: 10.1002/ange.201911510

    Multimodal x-ray and electron microscopy of the Allende meteorite
    journal, September 2019


    Quantitative Nanoscale 3D Imaging of Intergranular Corrosion of 304 Stainless Steel Using Hard X-Ray Nanoprobe
    journal, January 2019

    • Gill, Simerjeet K.; Ge, Mingyuan; Yan, Hanfei
    • Journal of The Electrochemical Society, Vol. 166, Issue 11
    • DOI: 10.1149/2.0401911jes

    Achieving diffraction-limited nanometer-scale X-ray point focus with two crossed multilayer Laue lenses: alignment challenges
    journal, January 2017

    • Yan, Hanfei; Huang, Xiaojing; Bouet, Nathalie
    • Optics Express, Vol. 25, Issue 21
    • DOI: 10.1364/oe.25.025234

    Full-field X-ray fluorescence microscope based on total-reflection advanced Kirkpatrick–Baez mirror optics
    journal, January 2019

    • Matsuyama, Satoshi; Yamada, Jumpei; Kohmura, Yoshiki
    • Optics Express, Vol. 27, Issue 13
    • DOI: 10.1364/oe.27.018318

    High resolution tip-tilt positioning system for a next generation MLL-based x-ray microscope
    journal, November 2017

    • Xu, Weihe; Schlossberger, Noah; Xu, Wei
    • Measurement Science and Technology, Vol. 28, Issue 12
    • DOI: 10.1088/1361-6501/aa8916

    Multi-slice ptychography with large numerical aperture multilayer Laue lenses
    journal, January 2018


    Development and characterization of monolithic multilayer Laue lens nanofocusing optics
    journal, June 2016

    • Nazaretski, E.; Xu, W.; Bouet, N.
    • Applied Physics Letters, Vol. 108, Issue 26
    • DOI: 10.1063/1.4955022

    In-situ synchrotron x-ray studies of the microstructure and stability of In 2 O 3 epitaxial films
    journal, October 2017

    • Highland, M. J.; Hruszkewycz, S. O.; Fong, D. D.
    • Applied Physics Letters, Vol. 111, Issue 16
    • DOI: 10.1063/1.4997773

    Multilayer Laue Lens: A Brief History and Current Status
    journal, July 2016