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Title: Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution

Here, we developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [3] ;  [3] ;  [1] ;  [1] ;  [1] ;  [1] ;  [4] ;  [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Diamond Light Source Ltd., Oxfordshire (United Kingdom)
  3. Univ. College London, London (United Kingdom); Rutherford Appleton Lab., Oxfordshire (United Kingdom)
  4. Brookhaven National Lab. (BNL), Upton, NY (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; fluorescence imaging; imaging techniques
OSTI Identifier:
1248377

Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, and Chu, Yong S.. Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution. United States: N. p., Web. doi:10.1038/srep20112.
Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, & Chu, Yong S.. Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution. United States. doi:10.1038/srep20112.
Yan, Hanfei, Nazaretski, Evgeny, Lauer, Kenneth R., Huang, Xiaojing, Wagner, Ulrich, Rau, Christoph, Yusuf, Mohammed, Robinson, Ian K., Kalbfleisch, Sebastian, Li, Li, Bouet, Nathalie, Zhou, Juan, Conley, Ray, and Chu, Yong S.. 2016. "Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution". United States. doi:10.1038/srep20112. https://www.osti.gov/servlets/purl/1248377.
@article{osti_1248377,
title = {Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution},
author = {Yan, Hanfei and Nazaretski, Evgeny and Lauer, Kenneth R. and Huang, Xiaojing and Wagner, Ulrich and Rau, Christoph and Yusuf, Mohammed and Robinson, Ian K. and Kalbfleisch, Sebastian and Li, Li and Bouet, Nathalie and Zhou, Juan and Conley, Ray and Chu, Yong S.},
abstractNote = {Here, we developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.},
doi = {10.1038/srep20112},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {2016},
month = {2}
}