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Title: Elastic properties of protein functionalized nanoporous polymer films

Abstract

Retaining the conformational structure and bioactivity of immobilized proteins is important for biosensor designs and drug delivery systems. Confined environments often lead to changes in conformation and functions of proteins. In this study, lysozyme is chemically tethered into nanopores of polystyrene thin films, and submicron pores in poly(methyl methacrylate) films are functionalized with streptavidin. Nanoindentation experiments show that stiffness of streptavidin increases with decreasing submicron pore sizes. Lysozymes in polystyrene nanopores are found to behave stiffer than the submicron pore sizes and still retain their specific bioactivity relative to the proteins on flat surfaces. Lastly, our results show that protein functionalized ordered nanoporous polystyrene/poly(methyl methacrylate) films present heterogeneous elasticity and can be used to study interactions between free proteins and designed surfaces.

Authors:
;  [1];  [1]
  1. Stevens Institute of Technology, Hoboken, NJ (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1246790
Report Number(s):
BNL-111949-2016-JA
Journal ID: ISSN 0743-7463; R&D Project: 16062/16062; KC0403020
Grant/Contract Number:  
SC00112704
Resource Type:
Accepted Manuscript
Journal Name:
Langmuir
Additional Journal Information:
Journal Volume: 32; Journal Issue: 1; Journal ID: ISSN 0743-7463
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; nanoporous polystyrene; poly(methyl methacrylate); thin film; lysozyme; stiffness; activity; heterogeneous surface

Citation Formats

Charles T. Black, Wang, Haoyu, and Akcora, Pinar. Elastic properties of protein functionalized nanoporous polymer films. United States: N. p., 2015. Web. https://doi.org/10.1021/acs.langmuir.5b04334.
Charles T. Black, Wang, Haoyu, & Akcora, Pinar. Elastic properties of protein functionalized nanoporous polymer films. United States. https://doi.org/10.1021/acs.langmuir.5b04334
Charles T. Black, Wang, Haoyu, and Akcora, Pinar. Wed . "Elastic properties of protein functionalized nanoporous polymer films". United States. https://doi.org/10.1021/acs.langmuir.5b04334. https://www.osti.gov/servlets/purl/1246790.
@article{osti_1246790,
title = {Elastic properties of protein functionalized nanoporous polymer films},
author = {Charles T. Black and Wang, Haoyu and Akcora, Pinar},
abstractNote = {Retaining the conformational structure and bioactivity of immobilized proteins is important for biosensor designs and drug delivery systems. Confined environments often lead to changes in conformation and functions of proteins. In this study, lysozyme is chemically tethered into nanopores of polystyrene thin films, and submicron pores in poly(methyl methacrylate) films are functionalized with streptavidin. Nanoindentation experiments show that stiffness of streptavidin increases with decreasing submicron pore sizes. Lysozymes in polystyrene nanopores are found to behave stiffer than the submicron pore sizes and still retain their specific bioactivity relative to the proteins on flat surfaces. Lastly, our results show that protein functionalized ordered nanoporous polystyrene/poly(methyl methacrylate) films present heterogeneous elasticity and can be used to study interactions between free proteins and designed surfaces.},
doi = {10.1021/acs.langmuir.5b04334},
journal = {Langmuir},
number = 1,
volume = 32,
place = {United States},
year = {2015},
month = {12}
}

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Cited by: 4 works
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