Elastic properties of protein functionalized nanoporous polymer films

Wang, Haoyu; Akcora, Pinar

doi:10.1021/acs.langmuir.5b04334

Title: Elastic properties of protein functionalized nanoporous polymer films

Abstract

Retaining the conformational structure and bioactivity of immobilized proteins is important for biosensor designs and drug delivery systems. Confined environments often lead to changes in conformation and functions of proteins. In this study, lysozyme is chemically tethered into nanopores of polystyrene thin films, and submicron pores in poly(methyl methacrylate) films are functionalized with streptavidin. Nanoindentation experiments show that stiffness of streptavidin increases with decreasing submicron pore sizes. Lysozymes in polystyrene nanopores are found to behave stiffer than the submicron pore sizes and still retain their specific bioactivity relative to the proteins on flat surfaces. Lastly, our results show that protein functionalized ordered nanoporous polystyrene/poly(methyl methacrylate) films present heterogeneous elasticity and can be used to study interactions between free proteins and designed surfaces.

Authors:

Charles T. Black; Wang, Haoyu ^[1]; Akcora, Pinar ^[1]

Stevens Institute of Technology, Hoboken, NJ (United States)

Publication Date:: 2015-12-16

Research Org.:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1246790

Report Number(s):: BNL-111949-2016-JA
Journal ID: ISSN 0743-7463; R&D Project: 16062/16062; KC0403020

Grant/Contract Number:: SC00112704

Resource Type:: Accepted Manuscript

Journal Name:: Langmuir

Additional Journal Information:: Journal Volume: 32; Journal Issue: 1; Journal ID: ISSN 0743-7463

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Subject:: 36 MATERIALS SCIENCE; nanoporous polystyrene; poly(methyl methacrylate); thin film; lysozyme; stiffness; activity; heterogeneous surface

Citation Formats


                    Charles T. Black, Wang, Haoyu, and Akcora, Pinar. Elastic properties of protein functionalized nanoporous polymer films.  United States: N. p., 2015. 
Web.  doi:10.1021/acs.langmuir.5b04334.

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                    Charles T. Black, Wang, Haoyu, & Akcora, Pinar. Elastic properties of protein functionalized nanoporous polymer films.  United States.  https://doi.org/10.1021/acs.langmuir.5b04334

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                    Charles T. Black, Wang, Haoyu, and Akcora, Pinar. Wed .  
"Elastic properties of protein functionalized nanoporous polymer films".  United States.  https://doi.org/10.1021/acs.langmuir.5b04334.  https://www.osti.gov/servlets/purl/1246790.

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@article{osti_1246790,

  title        = {Elastic properties of protein functionalized nanoporous polymer films},

  author       = {Charles T. Black and Wang, Haoyu and Akcora, Pinar},

  abstractNote = {Retaining the conformational structure and bioactivity of immobilized proteins is important for biosensor designs and drug delivery systems. Confined environments often lead to changes in conformation and functions of proteins. In this study, lysozyme is chemically tethered into nanopores of polystyrene thin films, and submicron pores in poly(methyl methacrylate) films are functionalized with streptavidin. Nanoindentation experiments show that stiffness of streptavidin increases with decreasing submicron pore sizes. Lysozymes in polystyrene nanopores are found to behave stiffer than the submicron pore sizes and still retain their specific bioactivity relative to the proteins on flat surfaces. Lastly, our results show that protein functionalized ordered nanoporous polystyrene/poly(methyl methacrylate) films present heterogeneous elasticity and can be used to study interactions between free proteins and designed surfaces.},

  doi          = {10.1021/acs.langmuir.5b04334},

  journal      = {Langmuir},

  number       = 1,

  volume       = 32,

  place        = {United States},

  year         = {2015},

  month        = {12}

}

Copy to clipboard

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https://doi.org/10.1021/acs.langmuir.5b04334

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Title: Elastic properties of protein functionalized nanoporous polymer films

Abstract

Citation Formats

Surface-modified nanoparticles as a new, versatile, and mechanically robust nonadhesive coating: Suppression of protein adsorption and bacterial adhesion journal, December 2009

Surface chemistry control of monocyte and macrophage adhesion, morphology, and fusion journal, January 2000

Protein analysis on a proteomic scale journal, March 2003

Conformational changes of enzymes upon immobilisation journal, January 2013

Enzymes on material surfaces journal, May 2012

Monolayer-Controlled Substrate Selectivity Using Noncovalent Enzyme−Nanoparticle Conjugates journal, October 2004

Enzyme-responsive nanoparticles for drug release and diagnostics journal, August 2012

Parallel tempering Monte Carlo simulations of lysozyme orientation on charged surfaces journal, February 2010

Activity of an enzyme immobilized on polyelectrolyte multilayers journal, January 2011

Protein Interactions with Polymer Coatings and Biomaterials journal, July 2014

Tunable Inhibition and Denaturation of α-Chymotrypsin with Amino Acid-Functionalized Gold Nanoparticles journal, September 2005

Interpretation of Protein Adsorption: Surface-Induced Conformational Changes journal, June 2005

Protein Adsorption on Model Surfaces with Controlled Nanotopography and Chemistry journal, February 2002

Enhancement of Protein Adsorption Induced by Surface Roughness journal, December 2006

Chemical Strategies for Generating Protein Biochips journal, December 2008

Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance journal, February 2011

Improvement of enzyme activity, stability and selectivity via immobilization techniques journal, May 2007

Facile Immobilization of Biomolecules onto Various Surfaces Using Epoxide-Containing Antibiofouling Polymers journal, February 2012

Control of Lysozyme Adsorption by pH on Surfaces Modified with Polyampholyte Brushes journal, January 2014

Site-directed and random immobilization of subtilisin on functionalized membranes: Activity determination in aqueous and organic media journal, December 1998

Structural origins of high-affinity biotin binding to streptavidin journal, January 1989

Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin–biotin attachment: a comparative study journal, July 2004

DDI-μFIA-A Readily Configurable Microarray-Fluorescence Immunoassay Based on DNA-Directed Immobilization of Proteins journal, April 2004

Control of Specific Attachment of Proteins by Adsorption of Polymer Layers journal, December 2006

Emerging applications of stimuli-responsive polymer materials journal, January 2010

Biocompatible and biodegradable phosphorylcholine ionomers with reduced protein adsorption and cell adhesion journal, January 2006

Protein-Resistant Polymer Coatings on Silicon Oxide by Surface-Initiated Atom Transfer Radical Polymerization journal, April 2006

Synthetic Hydrophilic Materials with Tunable Strength and a Range of Hydrophobic Interactions journal, June 2010

A Random Sequential Adsorption Model for Protein Adsorption to Surfaces Functionalized with Poly(ethylene oxide) journal, April 2009

Influence of PEG Architecture on Protein Adsorption and Conformation journal, December 2005

Manipulating Protein Adsorption using a Patchy Protein-Resistant Brush journal, July 2010

Strong Resistance of Phosphorylcholine Self-Assembled Monolayers to Protein Adsorption: Insights into Nonfouling Properties of Zwitterionic Materials journal, October 2005

Elastic Properties of a Protein–Polymer-Grafted Surface journal, February 2012

Enzymes immobilized in mesoporous silica: A physical–chemical perspective journal, March 2014

Function, Structure, and Stability of Enzymes Confined in Agarose Gels journal, January 2014

Stability of Proteins Inside a Hydrophobic Cavity journal, July 2013

Protein Stability in Nanocages: A Novel Approach for Influencing Protein Stability by Molecular Confinement journal, February 2004

Effects of surface curvature and surface chemistry on the structure and activity of proteins adsorbed in nanopores journal, January 2011

Mechanistic and Structural Features of Protein Adsorption onto Mesoporous Silicates journal, July 2002

Pore-Size Dependent Protein Adsorption and Protection from Proteolytic Hydrolysis in Tailored Mesoporous Silica Particles journal, October 2013

Comparison of different procedures of biotin immobilization on gold for the molecular recognition of avidin: an FT-IRRAS study: Biotin immobilization on Au for avidin recognition journal, August 2002

Thiol-Ene Click Chemistry journal, February 2010

Scanning probe anodization: Nanolithography using thin films of anodically oxidizable materials as resists journal, May 1996

Measuring elasticity of biological materials by atomic force microscopy journal, June 1998

Atomic force microscopy probing of cell elasticity journal, December 2007

Measurement of nanomechanical properties of biomolecules using atomic force microscopy journal, February 2012

Force-distance curves by atomic force microscopy journal, January 1999

Elasticity mapping of living fibroblasts by AFM and immunofluorescence observation of the cytoskeleton journal, February 2000

The Effects of Elastic Fiber Protein Insufficiency and Treatment on the Modulus of Arterial Smooth Muscle Cells journal, February 2014

Imaging adhesion forces and elasticity of lysozyme adsorbed on mica with the atomic force microscope journal, October 1994

Micromechanical Properties of Elastic Polymeric Materials As Probed by Scanning Force Microscopy journal, May 1998

A General Equation for Fitting Contact Area and Friction vs Load Measurements journal, March 1999

Calculation of Young's Modulus Value by Means of AFM journal, January 2011

Mapping hydration dynamics around a protein surface journal, November 2007

Water rotation barriers on protein molecular surfaces journal, February 2015

Hydrophobic Hydration Is an Important Source of Elasticity in Elastin-Based Biopolymers journal, December 2001

Sound Velocity and Elasticity of Tetragonal Lysozyme Crystals by Brillouin Spectroscopy journal, November 2003

Surface Tailoring for Controlled Protein Adsorption: Effect of Topography at the Nanometer Scale and Chemistry journal, March 2006

Block Copolymer Modified Surfaces for Conjugation of Biomacromolecules with Control of Quantity and Activity journal, January 2013

Synthesis of Fluorescent Polythiophene Dots journal, August 2018

Surface-modified nanoparticles as a new, versatile, and mechanically robust nonadhesive coating: Suppression of protein adsorption and bacterial adhesion
journal, December 2009

Surface chemistry control of monocyte and macrophage adhesion, morphology, and fusion
journal, January 2000

Protein analysis on a proteomic scale
journal, March 2003

Conformational changes of enzymes upon immobilisation
journal, January 2013

Enzymes on material surfaces
journal, May 2012

Monolayer-Controlled Substrate Selectivity Using Noncovalent Enzyme−Nanoparticle Conjugates
journal, October 2004

Enzyme-responsive nanoparticles for drug release and diagnostics
journal, August 2012

Parallel tempering Monte Carlo simulations of lysozyme orientation on charged surfaces
journal, February 2010

Activity of an enzyme immobilized on polyelectrolyte multilayers
journal, January 2011

Protein Interactions with Polymer Coatings and Biomaterials
journal, July 2014

Tunable Inhibition and Denaturation of α-Chymotrypsin with Amino Acid-Functionalized Gold Nanoparticles
journal, September 2005

Interpretation of Protein Adsorption: Surface-Induced Conformational Changes
journal, June 2005

Protein Adsorption on Model Surfaces with Controlled Nanotopography and Chemistry
journal, February 2002

Enhancement of Protein Adsorption Induced by Surface Roughness
journal, December 2006

Chemical Strategies for Generating Protein Biochips
journal, December 2008

Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance
journal, February 2011

Improvement of enzyme activity, stability and selectivity via immobilization techniques
journal, May 2007

Facile Immobilization of Biomolecules onto Various Surfaces Using Epoxide-Containing Antibiofouling Polymers
journal, February 2012

Control of Lysozyme Adsorption by pH on Surfaces Modified with Polyampholyte Brushes
journal, January 2014

Site-directed and random immobilization of subtilisin on functionalized membranes: Activity determination in aqueous and organic media
journal, December 1998

Structural origins of high-affinity biotin binding to streptavidin
journal, January 1989

Performance of antibody microarrays fabricated by either DNA-directed immobilization, direct spotting, or streptavidin–biotin attachment: a comparative study
journal, July 2004

DDI-μFIA-A Readily Configurable Microarray-Fluorescence Immunoassay Based on DNA-Directed Immobilization of Proteins
journal, April 2004

Control of Specific Attachment of Proteins by Adsorption of Polymer Layers
journal, December 2006

Emerging applications of stimuli-responsive polymer materials
journal, January 2010

Biocompatible and biodegradable phosphorylcholine ionomers with reduced protein adsorption and cell adhesion
journal, January 2006

Protein-Resistant Polymer Coatings on Silicon Oxide by Surface-Initiated Atom Transfer Radical Polymerization
journal, April 2006

Synthetic Hydrophilic Materials with Tunable Strength and a Range of Hydrophobic Interactions
journal, June 2010

A Random Sequential Adsorption Model for Protein Adsorption to Surfaces Functionalized with Poly(ethylene oxide)
journal, April 2009

Influence of PEG Architecture on Protein Adsorption and Conformation
journal, December 2005

Manipulating Protein Adsorption using a Patchy Protein-Resistant Brush
journal, July 2010

Strong Resistance of Phosphorylcholine Self-Assembled Monolayers to Protein Adsorption: Insights into Nonfouling Properties of Zwitterionic Materials
journal, October 2005

Elastic Properties of a Protein–Polymer-Grafted Surface
journal, February 2012

Enzymes immobilized in mesoporous silica: A physical–chemical perspective
journal, March 2014

Function, Structure, and Stability of Enzymes Confined in Agarose Gels
journal, January 2014

Stability of Proteins Inside a Hydrophobic Cavity
journal, July 2013

Protein Stability in Nanocages: A Novel Approach for Influencing Protein Stability by Molecular Confinement
journal, February 2004

Effects of surface curvature and surface chemistry on the structure and activity of proteins adsorbed in nanopores
journal, January 2011

Mechanistic and Structural Features of Protein Adsorption onto Mesoporous Silicates
journal, July 2002

Pore-Size Dependent Protein Adsorption and Protection from Proteolytic Hydrolysis in Tailored Mesoporous Silica Particles
journal, October 2013

Comparison of different procedures of biotin immobilization on gold for the molecular recognition of avidin: an FT-IRRAS study: Biotin immobilization on Au for avidin recognition
journal, August 2002

Thiol-Ene Click Chemistry
journal, February 2010

Scanning probe anodization: Nanolithography using thin films of anodically oxidizable materials as resists
journal, May 1996

Measuring elasticity of biological materials by atomic force microscopy
journal, June 1998

Atomic force microscopy probing of cell elasticity
journal, December 2007

Measurement of nanomechanical properties of biomolecules using atomic force microscopy
journal, February 2012

Force-distance curves by atomic force microscopy
journal, January 1999

Elasticity mapping of living fibroblasts by AFM and immunofluorescence observation of the cytoskeleton
journal, February 2000

The Effects of Elastic Fiber Protein Insufficiency and Treatment on the Modulus of Arterial Smooth Muscle Cells
journal, February 2014

Imaging adhesion forces and elasticity of lysozyme adsorbed on mica with the atomic force microscope
journal, October 1994

Micromechanical Properties of Elastic Polymeric Materials As Probed by Scanning Force Microscopy
journal, May 1998

A General Equation for Fitting Contact Area and Friction vs Load Measurements
journal, March 1999

Calculation of Young's Modulus Value by Means of AFM
journal, January 2011

Mapping hydration dynamics around a protein surface
journal, November 2007

Water rotation barriers on protein molecular surfaces
journal, February 2015

Hydrophobic Hydration Is an Important Source of Elasticity in Elastin-Based Biopolymers
journal, December 2001

Sound Velocity and Elasticity of Tetragonal Lysozyme Crystals by Brillouin Spectroscopy
journal, November 2003

Surface Tailoring for Controlled Protein Adsorption: Effect of Topography at the Nanometer Scale and Chemistry
journal, March 2006

Block Copolymer Modified Surfaces for Conjugation of Biomacromolecules with Control of Quantity and Activity
journal, January 2013

Synthesis of Fluorescent Polythiophene Dots
journal, August 2018