In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells
Abstract
The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast Hansenula polymorpha is reported and characterized. Furthermore, the observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of in cellulo serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.
- Authors:
-
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); European Molecular Biology Lab. (EMBL), Heidelberg (Germany)
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
- Univ. of Groningen, Groningen (The Netherlands)
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Center for Ultrafast Imaging, Hamburg (Germany)
- Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. Medical Center Hamburg-Eppendorf, Hamburg (Germany)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1244994
- Alternate Identifier(s):
- OSTI ID: 1307374
- Report Number(s):
- AC02-76SF00515
Journal ID: ISSN 2052-2525; IUCRAJ; PII: S2052252515022927
- Grant/Contract Number:
- AC02-76SF00515
- Resource Type:
- Accepted Manuscript
- Journal Name:
- IUCrJ
- Additional Journal Information:
- Journal Volume: 3; Journal Issue: 2; Journal ID: ISSN 2052-2525
- Publisher:
- International Union of Crystallography
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; protein structure; X-ray crystallography; femtosecond studies; nanocrystals; free-electron laser; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Jakobi, Arjen J., Passon, Daniel M., Knoops, Kevin, Stellato, Francesco, Liang, Mengning, White, Thomas A., Seine, Thomas, Messerschmidt, Marc, Chapman, Henry N., and Wilmanns, Matthias. In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells. United States: N. p., 2016.
Web. doi:10.1107/S2052252515022927.
Jakobi, Arjen J., Passon, Daniel M., Knoops, Kevin, Stellato, Francesco, Liang, Mengning, White, Thomas A., Seine, Thomas, Messerschmidt, Marc, Chapman, Henry N., & Wilmanns, Matthias. In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells. United States. https://doi.org/10.1107/S2052252515022927
Jakobi, Arjen J., Passon, Daniel M., Knoops, Kevin, Stellato, Francesco, Liang, Mengning, White, Thomas A., Seine, Thomas, Messerschmidt, Marc, Chapman, Henry N., and Wilmanns, Matthias. Tue .
"In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells". United States. https://doi.org/10.1107/S2052252515022927. https://www.osti.gov/servlets/purl/1244994.
@article{osti_1244994,
title = {In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells},
author = {Jakobi, Arjen J. and Passon, Daniel M. and Knoops, Kevin and Stellato, Francesco and Liang, Mengning and White, Thomas A. and Seine, Thomas and Messerschmidt, Marc and Chapman, Henry N. and Wilmanns, Matthias},
abstractNote = {The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast Hansenula polymorpha is reported and characterized. Furthermore, the observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of in cellulo serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.},
doi = {10.1107/S2052252515022927},
journal = {IUCrJ},
number = 2,
volume = 3,
place = {United States},
year = {Tue Mar 01 00:00:00 EST 2016},
month = {Tue Mar 01 00:00:00 EST 2016}
}
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