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Title: Complete genome sequence of Methanospirillum hungatei type strain JF1

Methanospirillum hungatei strain JF1 (DSM 864) is a methane-producing archaeon and is the type species of the genus Methanospirillum, which belongs to the family Methanospirillaceae within the order Methanomicrobiales. Its genome was selected for sequencing due to its ability to utilize hydrogen and carbon dioxide and/or formate as a sole source of energy. Ecologically, M. hungatei functions as the hydrogen- and/or formate-using partner with many species of syntrophic bacteria. Its morphology is distinct from other methanogens with the ability to form long chains of cells (up to 100 m in length), which are enclosed within a sheath-like structure, and terminal cells with polar flagella. The genome of M. hungatei strain JF1 is the first completely sequenced genome of the family Methanospirillaceae, and it has a circular genome of 3,544,738 bp containing 3,239 protein coding and 68 RNA genes. Furthermore, the large genome of M. hungatei JF1 suggests the presence of unrecognized biochemical/physiological properties that likely extend to the other Methanospirillaceae and include the ability to form the unusual sheath-like structure and to successfully interact with syntrophic bacteria.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [2] ;  [4] ;  [3] ;  [2] ;  [2] ;  [5] ;  [6] ;  [7] ;  [7] ;  [8] ;  [9] ;  [10] ;  [3]
  1. Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics; Univ. of California, Los Angeles, CA (United States). UCLA-DOE Institute for Genomics and Proteomics
  2. Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics
  3. Univ. of Oklahoma, Norman, OK (United States)
  4. Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics; Univ. of Oklahoma, Norman, OK (United States)
  5. St. Petersburg State University, St. Petersburg (Russia); St. Petersburg Academic University, St. Petersburg (Russia)
  6. St. Petersburg State University, St. Petersburg (Russia)
  7. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  8. DOE Joint Genome Institute, Walnut Creek, CA (United States)
  9. DOE Joint Genome Institute, Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)
  10. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Report Number(s):
PNNL-SA-100776
Journal ID: ISSN 1944-3277; BM0101020
Grant/Contract Number:
AC05-76RL01830; FG02-96ER20214; FG03-86ER13498; FC02-02ER63421; AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Standards in Genomic Sciences
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1944-3277
Publisher:
BioMed Central
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Work for Others (WFO)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; syntrophy; biohydrogen; methanogeneis; biofuels; hydrogenase; formate dehydrogenase; bioenergetics; fatty acids; syntrophic partnerships; Methanomicrobiales; anaerobic; motile; methangenic; hydrogen; Methangenic archaea; formate
OSTI Identifier:
1243176
Alternate Identifier(s):
OSTI ID: 1265977