Crystal structure of a substrate-engaged SecY protein-translocation channel
Abstract
Hydrophobic signal sequences target secretory polypeptides to a protein-conducting channel formed by a heterotrimeric membrane protein complex, the prokaryotic SecY or eukaryotic Sec61 complex. How signal sequences are recognized is poorly understood, particularly because they are diverse in sequence and length. Structures of the inactive channel show that the largest subunit, SecY or Sec61α, consists of two halves that form an hourglass-shaped pore with a constriction in the middle of the membrane and a lateral gate that faces lipid. The cytoplasmic funnel is empty, while the extracellular funnel is filled with a plug domain. In bacteria, the SecY channel associates with the translating ribosome in co-translational translocation, and with the SecA ATPase in post-translational translocation. How a translocating polypeptide inserts into the channel is uncertain, as cryo-electron microscopy structures of the active channel have a relatively low resolution (~10 Å) or are of insufficient quality. Here we report a crystal structure of the active channel, assembled from SecY complex, the SecA ATPase, and a segment of a secretory protein fused into SecA. The translocating protein segment inserts into the channel as a loop, displacing the plug domain. The hydrophobic core of the signal sequence forms a helix that sits inmore »
- Authors:
-
- Howard Hughes Medical Institute and Harvard Medical School, Boston, MA (United States)
- The Rockefeller University and Howard Hughes Medical Inst., New York, NY (United States); Howard Hughes Medical Institute and Harvard Medical School, Boston, MA (United States)
- Whitehead Inst. for Biomedical Research, Cambridge, MA (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- National Inst. of Health
- OSTI Identifier:
- 1243126
- Grant/Contract Number:
- GM052586
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature (London)
- Additional Journal Information:
- Journal Name: Nature (London); Journal Volume: 531; Journal Issue: 7594; Journal ID: ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Protein translocation; X-ray crystallography
Citation Formats
Li, Long, Park, Eunyong, Ling, JingJing, Ingram, Jessica, Ploegh, Hidde, and Rapoport, Tom A. Crystal structure of a substrate-engaged SecY protein-translocation channel. United States: N. p., 2016.
Web. doi:10.1038/nature17163.
Li, Long, Park, Eunyong, Ling, JingJing, Ingram, Jessica, Ploegh, Hidde, & Rapoport, Tom A. Crystal structure of a substrate-engaged SecY protein-translocation channel. United States. https://doi.org/10.1038/nature17163
Li, Long, Park, Eunyong, Ling, JingJing, Ingram, Jessica, Ploegh, Hidde, and Rapoport, Tom A. Mon .
"Crystal structure of a substrate-engaged SecY protein-translocation channel". United States. https://doi.org/10.1038/nature17163. https://www.osti.gov/servlets/purl/1243126.
@article{osti_1243126,
title = {Crystal structure of a substrate-engaged SecY protein-translocation channel},
author = {Li, Long and Park, Eunyong and Ling, JingJing and Ingram, Jessica and Ploegh, Hidde and Rapoport, Tom A.},
abstractNote = {Hydrophobic signal sequences target secretory polypeptides to a protein-conducting channel formed by a heterotrimeric membrane protein complex, the prokaryotic SecY or eukaryotic Sec61 complex. How signal sequences are recognized is poorly understood, particularly because they are diverse in sequence and length. Structures of the inactive channel show that the largest subunit, SecY or Sec61α, consists of two halves that form an hourglass-shaped pore with a constriction in the middle of the membrane and a lateral gate that faces lipid. The cytoplasmic funnel is empty, while the extracellular funnel is filled with a plug domain. In bacteria, the SecY channel associates with the translating ribosome in co-translational translocation, and with the SecA ATPase in post-translational translocation. How a translocating polypeptide inserts into the channel is uncertain, as cryo-electron microscopy structures of the active channel have a relatively low resolution (~10 Å) or are of insufficient quality. Here we report a crystal structure of the active channel, assembled from SecY complex, the SecA ATPase, and a segment of a secretory protein fused into SecA. The translocating protein segment inserts into the channel as a loop, displacing the plug domain. The hydrophobic core of the signal sequence forms a helix that sits in a groove outside the lateral gate, while the following polypeptide segment intercalates into the gate. The carboxy (C)-terminal section of the polypeptide loop is located in the channel, surrounded by residues of the pore ring. Furthermore, during translocation, the hydrophobic segments of signal sequences, and probably bilayer-spanning domains of nascent membrane proteins, exit the lateral gate and dock at a specific site that faces the lipid phase.},
doi = {10.1038/nature17163},
journal = {Nature (London)},
number = 7594,
volume = 531,
place = {United States},
year = {Mon Mar 07 00:00:00 EST 2016},
month = {Mon Mar 07 00:00:00 EST 2016}
}
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Works referenced in this record:
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites
journal, January 1997
- Nielsen, H.; Engelbrecht, J.; Brunak, S.
- Protein Engineering Design and Selection, Vol. 10, Issue 1, p. 1-6
Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes
journal, September 2010
- Egea, P. F.; Stroud, R. M.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 40
Solving structures of protein complexes by molecular replacement with Phaser
journal, December 2006
- McCoy, Airlie J.
- Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 1
Visualization of a polytopic membrane protein during SecY-mediated membrane insertion
journal, June 2014
- Bischoff, Lukas; Wickles, Stephan; Berninghausen, Otto
- Nature Communications, Vol. 5, Issue 1
Conformational behavior of Escherichia coli OmpA signal peptides in membrane mimetic environments
journal, May 1993
- Rizo, Josep; Blanco, Francisco J.; Kobe, Bostjan
- Biochemistry, Vol. 32, Issue 18
Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY
journal, April 2005
- Cannon, Kurt S.; Or, Eran; Clemons, William M.
- Journal of Cell Biology, Vol. 169, Issue 2
Crystal Structures of SecYEG in Lipidic Cubic Phase Elucidate a Precise Resting and a Peptide-Bound State
journal, November 2015
- Tanaka, Yoshiki; Sugano, Yasunori; Takemoto, Mizuki
- Cell Reports, Vol. 13, Issue 8
Signal Sequence Recognition in Posttranslational Protein Transport across the Yeast ER Membrane
journal, September 1998
- Plath, Kathrin; Mothes, Walther; Wilkinson, Barrie M.
- Cell, Vol. 94, Issue 6
Structure of the SecY channel during initiation of protein translocation
journal, October 2013
- Park, Eunyong; Ménétret, Jean-François; Gumbart, James C.
- Nature, Vol. 506, Issue 7486
Structure of a complex of the ATPase SecA and the protein-translocation channel
journal, October 2008
- Zimmer, Jochen; Nam, Yunsun; Rapoport, Tom A.
- Nature, Vol. 455, Issue 7215
Mechanisms of Sec61/SecY-Mediated Protein Translocation Across Membranes
journal, June 2012
- Park, Eunyong; Rapoport, Tom A.
- Annual Review of Biophysics, Vol. 41, Issue 1
Fusion Partner Toolchest for the Stabilization and Crystallization of G Protein-Coupled Receptors
journal, June 2012
- Chun, Eugene; Thompson, Aaron A.; Liu, Wei
- Structure, Vol. 20, Issue 6
Version 1.2 of the Crystallography and NMR system
journal, October 2007
- Brunger, Axel T.
- Nature Protocols, Vol. 2, Issue 11
GPCR Engineering Yields High-Resolution Structural Insights into 2-Adrenergic Receptor Function
journal, October 2007
- Rosenbaum, D. M.; Cherezov, V.; Hanson, M. A.
- Science, Vol. 318, Issue 5854
Mapping an Interface of SecY (PrlA) and SecE (PrlG) by Using Synthetic Phenotypes and In Vivo Cross-Linking
journal, June 1999
- Harris, Chris R.; Silhavy, Thomas J.
- Journal of Bacteriology, Vol. 181, Issue 11
A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane
journal, July 1995
- Jungnickel, Berit; Rapoport, Tom A.
- Cell, Vol. 82, Issue 2
Bacterial protein translocation requires only one copy of the SecY complex in vivo
journal, August 2012
- Park, Eunyong; Rapoport, Tom A.
- The Journal of Cell Biology, Vol. 198, Issue 5
UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004
- Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
- Journal of Computational Chemistry, Vol. 25, Issue 13
The Sec61p Complex Mediates the Integration of a Membrane Protein by Allowing Lipid Partitioning of the Transmembrane Domain
journal, July 2000
- Heinrich, Sven U.; Mothes, Walther; Brunner, Josef
- Cell, Vol. 102, Issue 2
Preserving the membrane barrier for small molecules during bacterial protein translocation
journal, May 2011
- Park, Eunyong; Rapoport, Tom A.
- Nature, Vol. 473, Issue 7346
Engineering and characterization of a superfolder green fluorescent protein
journal, December 2005
- Pédelacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy
- Nature Biotechnology, Vol. 24, Issue 1, p. 79-88
Conformational Requirement of Signal Sequences Functioning in Yeast: Circular Dichroism and 1H Nuclear Magnetic Resonance Studies of Synthetic Peptides
dataset, January 1995
- Yamamoto, Yoshio; Ohkubo, Tadayasu; Kohara, Atuko
- Biological Magnetic Resonance Bank
The Plug Domain of the SecY Protein Stabilizes the Closed State of the Translocation Channel and Maintains a Membrane Seal
journal, May 2007
- Li, Weikai; Schulman, Sol; Boyd, Dana
- Molecular Cell, Vol. 26, Issue 4
Collaboration gets the most out of software
journal, September 2013
- Morin, Andrew; Eisenbraun, Ben; Key, Jason
- eLife, Vol. 2
The protein-conducting channel in the membrane of the endoplasmic reticulum is open laterally toward the lipid bilayer
journal, April 1995
- Martoglio, Bruno; Hofmann, Michael W.; Brunner, Josef
- Cell, Vol. 81, Issue 2
XDS
journal, January 2010
- Kabsch, Wolfgang
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
Three-dimensional structure of the bacterial protein-translocation complex SecYEG
journal, August 2002
- Breyton, Cécile; Haase, Winfried; Rapoport, Tom A.
- Nature, Vol. 418, Issue 6898
Three-dimensional structure of the bacterial protein-translocation complex SecYEG
journal, August 2002
- Breyton, Cécile; Haase, Winfried; Rapoport, Tom A.
- Nature, Vol. 418, Issue 6898
The Protein-Conducting Channel in the Membrane of the Endoplasmic Reticulum Is Open Laterally toward the Lipid Bilayer
text, January 2008
- Martoglio, Bruno; Hofmann, Michael W.; Brunner, Josef
- Heidelberg University Library
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
journal, August 2013
- Guimaraes, Carla P.; Witte, Martin D.; Theile, Christopher S.
- Nature Protocols, Vol. 8, Issue 9
Phasing at high resolution using Ta 6 Br 12 cluster
journal, February 2003
- Banumathi, Sankaran; Dauter, Miroslawa; Dauter, Zbigniew
- Acta Crystallographica Section D Biological Crystallography, Vol. 59, Issue 3
How good are my data and what is the resolution?
journal, June 2013
- Evans, Philip R.; Murshudov, Garib N.
- Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7
Version 1.2 of the Crystallography and NMR system
journal, October 2007
- Brunger, Axel T.
- Nature Protocols, Vol. 2, Issue 11
Structure of the Mammalian Ribosome-Sec61 Complex to 3.4 Å Resolution
journal, June 2014
- Voorhees, Rebecca M.; Fernández, Israel S.; Scheres, Sjors H. W.
- Cell, Vol. 157, Issue 7
Conformational requirement of signal sequences functioning in yeast: circular dichroism and proton nuclear magnetic resonance studies of synthetic peptides
journal, September 1990
- Yamamoto, Yoshio; Ohkubo, Tadayasu; Kohara, Atuko
- Biochemistry, Vol. 29, Issue 38
Structure and function of a membrane component SecDF that enhances protein export
journal, May 2011
- Tsukazaki, Tomoya; Mori, Hiroyuki; Echizen, Yuka
- Nature, Vol. 474, Issue 7350
Investigating the SecY plug movement at the SecYEG translocation channel
journal, September 2005
- Tam, Patrick C. K.; Maillard, Antoine P.; Chan, Kenneth K. Y.
- The EMBO Journal, Vol. 24, Issue 19
Tinkering with nature
journal, June 2011
- Rehling, Peter
- Nature Reviews Molecular Cell Biology, Vol. 12, Issue 7
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Apollo - University of Cambridge Repository
Preserving the membrane barrier for small molecules during bacterial protein translocation
journal, May 2011
- Park, Eunyong; Rapoport, Tom A.
- Nature, Vol. 473, Issue 7346
Cysteine-free rop: A four-helix bundle core mutant has wild-type stability and structure but dramatically different unfolding kinetics
journal, January 2010
- Hari, Sanjay B.; Byeon, Chang; Lavinder, Jason J.
- Protein Science, Vol. 19, Issue 4
Selection and identification of single domain antibody fragments from camel heavy-chain antibodies
journal, September 1997
- Arbabi Ghahroudi, M.; Desmyter, A.; Wyns, L.
- FEBS Letters, Vol. 414, Issue 3
Protein Translocation: The Sec61/SecYEG Translocon Caught in the Act
journal, April 2014
- Spiess, Martin
- Current Biology, Vol. 24, Issue 8
Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion
journal, February 2014
- Gogala, Marko; Becker, Thomas; Beatrix, Birgitta
- Nature, Vol. 506, Issue 7486
Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY
journal, April 2005
- Cannon, Kurt S.; Or, Eran; Clemons, William M.
- Journal of Cell Biology, Vol. 169, Issue 2
Fusion Partner Toolchest for the Stabilization and Crystallization of G Protein-Coupled Receptors
journal, June 2012
- Chun, Eugene; Thompson, Aaron A.; Liu, Wei
- Structure, Vol. 20, Issue 6
Recognition of transmembrane helices by the endoplasmic reticulum translocon
journal, January 2005
- Hessa, Tara; Kim, Hyun; Bihlmaier, Karl
- Nature, Vol. 433, Issue 7024
Structure of a complex of the ATPase SecA and the protein-translocation channel
journal, October 2008
- Zimmer, Jochen; Nam, Yunsun; Rapoport, Tom A.
- Nature, Vol. 455, Issue 7215
Structure of the SecY channel during initiation of protein translocation
journal, October 2013
- Park, Eunyong; Ménétret, Jean-François; Gumbart, James C.
- Nature, Vol. 506, Issue 7486
Alpaca (Lama pacos) as a convenient source of recombinant camelid heavy chain antibodies (VHHs)
journal, July 2007
- Maass, David R.; Sepulveda, Jorge; Pernthaner, Anton
- Journal of Immunological Methods, Vol. 324, Issue 1-2
Conformational Requirement of Signal Sequences Functioning in Yeast: Circular Dichroism and 1H Nuclear Magnetic Resonance Studies of Synthetic Peptides
dataset, January 1995
- Yamamoto, Yoshio; Ohkubo, Tadayasu; Kohara, Atuko
- Biological Magnetic Resonance Bank
Conformational behavior of Escherichia coli OmpA signal peptides in membrane mimetic environments
journal, May 1993
- Rizo, Josep; Blanco, Francisco J.; Kobe, Bostjan
- Biochemistry, Vol. 32, Issue 18
A “Push and Slide” Mechanism Allows Sequence-Insensitive Translocation of Secretory Proteins by the SecA ATPase
journal, June 2014
- Bauer, Benedikt W.; Shemesh, Tom; Chen, Yu
- Cell, Vol. 157, Issue 6
Conformational transition of Sec machinery inferred from bacterial SecYE structures
journal, October 2008
- Tsukazaki, Tomoya; Mori, Hiroyuki; Fukai, Shuya
- Nature, Vol. 455, Issue 7215
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites
journal, January 1997
- Nielsen, H.; Engelbrecht, J.; Brunak, S.
- Protein Engineering Design and Selection, Vol. 10, Issue 1, p. 1-6
HiLiDe—Systematic Approach to Membrane Protein Crystallization in Lipid and Detergent
journal, June 2011
- Gourdon, Pontus; Andersen, Jacob Lauwring; Hein, Kim Langmach
- Crystal Growth & Design, Vol. 11, Issue 6
Puzzle Plugged by Protein Pore Plasticity
journal, May 2007
- Minor, Daniel L.
- Molecular Cell, Vol. 26, Issue 4
Structure of the native Sec61 protein-conducting channel
journal, September 2015
- Pfeffer, Stefan; Burbaum, Laura; Unverdorben, Pia
- Nature Communications, Vol. 6, Issue 1
X-ray structure of a protein-conducting channel
journal, December 2003
- Berg, Bert van den; Clemons, William M.; Collinson, Ian
- Nature, Vol. 427, Issue 6969
PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome
journal, December 2019
- Sharma, Deepti; De Falco, Louis; Padavattan, Sivaraman
- Nature Communications, Vol. 10, Issue 1
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
journal, August 2013
- Guimaraes, Carla P.; Witte, Martin D.; Theile, Christopher S.
- Nature Protocols, Vol. 8, Issue 9
Features and development of Coot
journal, March 2010
- Emsley, P.; Lohkamp, B.; Scott, W. G.
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
Bacterial protein translocation requires only one copy of the SecY complex in vivo
journal, August 2012
- Park, Eunyong; Rapoport, Tom A.
- The Journal of Cell Biology, Vol. 198, Issue 5
Structure and mechanism of Escherichia coli type I signal peptidase
journal, August 2014
- Paetzel, Mark
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1843, Issue 8
Maximum-likelihood density modification
journal, August 2000
- Terwilliger, Thomas C.
- Acta Crystallographica Section D Biological Crystallography, Vol. 56, Issue 8
Structure of the Mammalian Ribosome-Sec61 Complex to 3.4 Å Resolution
journal, June 2014
- Voorhees, Rebecca M.; Fernández, Israel S.; Scheres, Sjors H. W.
- Cell, Vol. 157, Issue 7
Alpaca (Lama pacos) as a convenient source of recombinant camelid heavy chain antibodies (VHHs)
journal, July 2007
- Maass, David R.; Sepulveda, Jorge; Pernthaner, Anton
- Journal of Immunological Methods, Vol. 324, Issue 1-2
REFMAC 5 for the refinement of macromolecular crystal structures
journal, March 2011
- Murshudov, Garib N.; Skubák, Pavol; Lebedev, Andrey A.
- Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
A “Push and Slide” Mechanism Allows Sequence-Insensitive Translocation of Secretory Proteins by the SecA ATPase
journal, June 2014
- Bauer, Benedikt W.; Shemesh, Tom; Chen, Yu
- Cell, Vol. 157, Issue 6
Structure of the Sec61 channel opened by a signal sequence
journal, December 2015
- Voorhees, R. M.; Hegde, R. S.
- Science, Vol. 351, Issue 6268
UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004
- Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
- Journal of Computational Chemistry, Vol. 25, Issue 13
A second signal recognition event required for translocation into the endoplasmic reticulum
journal, July 1995
- Siegel, Vivian
- Cell, Vol. 82, Issue 2
The Protein-Conducting Channel in the Membrane of the Endoplasmic Reticulum Is Open Laterally toward the Lipid Bilayer
text, January 2008
- Martoglio, Bruno; Hofmann, Michael W.; Brunner, Josef
- Heidelberg University Library
Visualization of a polytopic membrane protein during SecY-mediated membrane insertion
journal, June 2014
- Bischoff, Lukas; Wickles, Stephan; Berninghausen, Otto
- Nature Communications, Vol. 5, Issue 1
Investigating the SecY plug movement at the SecYEG translocation channel
journal, September 2005
- Tam, Patrick C. K.; Maillard, Antoine P.; Chan, Kenneth K. Y.
- The EMBO Journal, Vol. 24, Issue 19
Functional and Nonfunctional LamB Signal Sequences Can Be Distinguished by Their Biophysical Properties
journal, October 1989
- McKnight, C. J.; Briggs, M. S.; Gierasch, L. M.
- Journal of Biological Chemistry, Vol. 264, Issue 29
Maturation of Escherichia coli maltose-binding protein by signal peptidase I in vivo. Sequence requirements for efficient processing and demonstration of an alternate cleavage site.
journal, February 1990
- Fikes, J. D.; Barkocy-Gallagher, G. A.; Klapper, D. G.
- Journal of Biological Chemistry, Vol. 265, Issue 6
Works referencing / citing this record:
Structure of the post-translational protein translocation machinery of the ER membrane
journal, December 2018
- Wu, Xudong; Cabanos, Cerrone; Rapoport, Tom A.
- Nature, Vol. 566, Issue 7742
Structural considerations of folded protein import through the chloroplast TOC / TIC translocons
journal, March 2019
- Ganesan, Iniyan; Theg, Steven M.
- FEBS Letters, Vol. 593, Issue 6
Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates
journal, December 2019
- da Silva, Ronni A. G.; Karlyshev, Andrey V.; Oldfield, Neil J.
- Frontiers in Microbiology, Vol. 10
Structure and topology around the cleavage site regulate post-translational cleavage of the HIV-1 gp160 signal peptide
journal, July 2017
- Snapp, Erik Lee; McCaul, Nicholas; Quandte, Matthias
- eLife, Vol. 6
A central cavity within the holo-translocon suggests a mechanism for membrane protein insertion.
journalarticle, January 2016
- Botte, Mathieu; Zaccai, Nathan R.; Nijeholt, Jelger Lycklama À
- Springer Science and Business Media LLC
Use of a sequential high throughput screening assay to identify novel inhibitors of the eukaryotic SRP-Sec61 targeting/translocation pathway
journal, December 2018
- Klein, Wolfgang; Rutz, Claudia; Eckhard, Jamina
- PLOS ONE, Vol. 13, Issue 12
Interaction mapping of the Sec61 translocon identifies two Sec61α regions interacting with hydrophobic segments in translocating chains
journal, September 2018
- Kida, Yuichiro; Sakaguchi, Masao
- Journal of Biological Chemistry, Vol. 293, Issue 44
Structural Basis of the Sec Translocon and YidC Revealed Through X-ray Crystallography
journal, April 2019
- Tsukazaki, Tomoya
- The Protein Journal, Vol. 38, Issue 3
Archaeal cell surface biogenesis
journal, June 2018
- Pohlschroder, Mechthild; Pfeiffer, Friedhelm; Schulze, Stefan
- FEMS Microbiology Reviews, Vol. 42, Issue 5
The molecular mechanism of cotranslational membrane protein recognition and targeting by SecA
journal, September 2019
- Wang, Shuai; Jomaa, Ahmad; Jaskolowski, Mateusz
- Nature Structural & Molecular Biology, Vol. 26, Issue 10
SecY-SecA fusion protein retains the ability to mediate protein transport
journal, August 2017
- Sugano, Yasunori; Furukawa, Arata; Nureki, Osamu
- PLOS ONE, Vol. 12, Issue 8
Post-Translational Protein Transport by the Sec Complex
journal, June 2019
- Allen, William J.; Collinson, Ian; Römisch, Karin
- Trends in Biochemical Sciences, Vol. 44, Issue 6
Structurally detailed coarse-grained model for Sec-facilitated co-translational protein translocation and membrane integration
journal, March 2017
- Niesen, Michiel J. M.; Wang, Connie Y.; Van Lehn, Reid C.
- PLOS Computational Biology, Vol. 13, Issue 3
Insertion of proteins and lipopolysaccharide into the bacterial outer membrane
journal, June 2017
- Botos, Istvan; Noinaj, Nicholas; Buchanan, Susan K.
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 372, Issue 1726
Use of a sequential high throughput screening assay to identify novel inhibitors of the eukaryotic SRP-Sec61 targeting/translocation pathway
journal, December 2018
- Klein, Wolfgang; Rutz, Claudia; Eckhard, Jamina
- PLOS ONE, Vol. 13, Issue 12
Driving Forces of Translocation Through Bacterial Translocon SecYEG
journal, January 2018
- Knyazev, Denis G.; Kuttner, Roland; Zimmermann, Mirjam
- The Journal of Membrane Biology, Vol. 251, Issue 3
Quality control of nonstop membrane proteins at the ER membrane and in the cytosol
journal, August 2016
- Arakawa, Shunsuke; Yunoki, Kaori; Izawa, Toshiaki
- Scientific Reports, Vol. 6, Issue 1
An alternate mode of oligomerization for E. coli SecA
journal, September 2017
- Yazdi, Aliakbar Khalili; Vezina, Grant C.; Shilton, Brian H.
- Scientific Reports, Vol. 7, Issue 1
Structure-based working model of SecDF, a proton-driven bacterial protein translocation factor
journal, April 2018
- Tsukazaki, Tomoya
- FEMS Microbiology Letters, Vol. 365, Issue 12
HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon
journal, July 2019
- Ahdash, Zainab; Pyle, Euan; Allen, William John
- eLife, Vol. 8
Structure of the posttranslational Sec protein-translocation channel complex from yeast
journal, December 2018
- Itskanov, Samuel; Park, Eunyong
- Science, Vol. 363, Issue 6422
Structure of the substrate-engaged SecA-SecY protein translocation machine
journal, June 2019
- Ma, Chengying; Wu, Xiaofei; Sun, Dongjie
- Nature Communications, Vol. 10, Issue 1
Tracking Proteins Secreted by Bacteria: What's in the Toolbox?
journal, May 2017
- Maffei, Benoit; Francetic, Olivera; Subtil, Agathe
- Frontiers in Cellular and Infection Microbiology, Vol. 7
Archaeal cell surface biogenesis
journal, June 2018
- Pohlschroder, Mechthild; Pfeiffer, Friedhelm; Schulze, Stefan
- FEMS Microbiology Reviews, Vol. 42, Issue 5
A clearer picture of the ER translocon complex
journal, February 2020
- Gemmer, Max; Förster, Friedrich
- Journal of Cell Science, Vol. 133, Issue 3
Molecular Mimicry of SecA and Signal Recognition Particle Binding to the Bacterial Ribosome
journal, August 2019
- Knüpffer, Lara; Fehrenbach, Clara; Denks, Kärt
- mBio, Vol. 10, Issue 4
Structure of the substrate-engaged SecA-SecY protein translocation machine
journal, June 2019
- Ma, Chengying; Wu, Xiaofei; Sun, Dongjie
- Nature Communications, Vol. 10, Issue 1
Protein translocation by the SecA ATPase occurs by a power‐stroke mechanism
journal, March 2019
- Catipovic, Marco A.; Bauer, Benedikt W.; Loparo, Joseph J.
- The EMBO Journal, Vol. 38, Issue 9
ATP-induced asymmetric pre-protein folding as a driver of protein translocation through the Sec machinery
journal, January 2019
- Corey, Robin A.; Ahdash, Zainab; Shah, Anokhi
- eLife, Vol. 8
EMC Is Required to Initiate Accurate Membrane Protein Topogenesis
journal, November 2018
- Chitwood, Patrick J.; Juszkiewicz, Szymon; Guna, Alina
- Cell, Vol. 175, Issue 6
Voltage Sensing in Bacterial Protein Translocation
text, January 2020
- Knyazev, Denis G.; Kuttner, Roland; Bondar, Ana-Nicoleta
- Freie Universität Berlin
Atomic structures of anthrax toxin protective antigen channels bound to partially unfolded lethal and edema factors
journal, February 2020
- Hardenbrook, Nathan J.; Liu, Shiheng; Zhou, Kang
- Nature Communications, Vol. 11, Issue 1
Molecular Mimicry of SecA and Signal Recognition Particle Binding to the Bacterial Ribosome
journal, August 2019
- Knüpffer, Lara; Fehrenbach, Clara; Denks, Kärt
- mBio, Vol. 10, Issue 4
The way is the goal: how SecA transports proteins across the cytoplasmic membrane in bacteria
journal, April 2018
- Cranford-Smith, Tamar; Huber, Damon
- FEMS Microbiology Letters, Vol. 365, Issue 11
Protein export through the bacterial Sec pathway
journal, November 2016
- Tsirigotaki, Alexandra; De Geyter, Jozefien; Šoštaric´, Nikolina
- Nature Reviews Microbiology, Vol. 15, Issue 1
Voltage Sensing in Bacterial Protein Translocation
text, January 2020
- Knyazev, Denis G.; Kuttner, Roland; Bondar, Ana-Nicoleta
- Freie Universität Berlin
The Dynamic ATP-Driven Mechanism of Bacterial Protein Translocation and the Critical Role of Phospholipids
journal, June 2019
- Collinson, Ian
- Frontiers in Microbiology, Vol. 10
Driving Forces of Translocation Through Bacterial Translocon SecYEG
journal, January 2018
- Knyazev, Denis G.; Kuttner, Roland; Zimmermann, Mirjam
- The Journal of Membrane Biology, Vol. 251, Issue 3
Dynamic action of the Sec machinery during initiation, protein translocation and termination
journal, June 2018
- Fessl, Tomas; Watkins, Daniel; Oatley, Peter
- eLife, Vol. 7
Protein Translocation: SecA–SecY Conformational Crosstalk Opens Channel
journal, September 2016
- Kuhn, Andreas; Dalbey, Ross E.
- Current Biology, Vol. 26, Issue 17
Voltage Sensing in Bacterial Protein Translocation
journal, January 2020
- Knyazev, Denis G.; Kuttner, Roland; Bondar, Ana-Nicoleta
- Biomolecules, Vol. 10, Issue 1
Protein Transport Across the Bacterial Plasma Membrane by the Sec Pathway
journal, May 2019
- Smets, Dries; Loos, Maria S.; Karamanou, Spyridoula
- The Protein Journal, Vol. 38, Issue 3
Two-way communication between SecY and SecA suggests a Brownian ratchet mechanism for protein translocation
journal, May 2016
- Allen, William John; Corey, Robin Adam; Oatley, Peter
- eLife, Vol. 5
ZMPSTE24 missense mutations that cause progeroid diseases decrease prelamin A cleavage activity and/or protein stability
journal, May 2018
- Spear, Eric D.; Hsu, Erh-Ting; Nie, Laiyin
- Disease Models & Mechanisms, Vol. 11, Issue 7