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Title: Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta

Abstract

Sexual reproduction is an obligate phase in the life cycle of most eukaryotes. Meiosis varies among organisms, which is reflected by the variability of the gene set associated to the process. Diatoms are unicellular organisms that belong to the stramenopile clade and have unique life cycles that can include a sexual phase. The exploration of five diatom genomes and one diatom transcriptome led to the identification of 42 genes potentially involved in meiosis. While these include the majority of known meiosis-related genes, several meiosis-specific genes, including DMC1, could not be identified. Furthermore, phylogenetic analyses supported gene identification and revealed ancestral loss and recent expansion in the RAD51 family in diatoms. The two sexual species Pseudo-nitzschia multistriata and Seminavis robusta were used to explore the expression of meiosis-related genes: RAD21, SPO11-2, RAD51-A, RAD51-B and RAD51-C were upregulated during meiosis, whereas other paralogs in these families showed no differential expression patterns, suggesting that they may play a role during vegetative divisions. An almost identical toolkit is shared among Pseudo-nitzschia multiseries and Fragilariopsis cylindrus, as well as two species for which sex has not been observed, Phaeodactylum tricornutum and Thalassiosira pseudonana, suggesting that these two may retain a facultative sexual phase. Lastly, ourmore » results reveal the conserved meiotic toolkit in six diatom species and indicate that Stramenopiles share major modifications of canonical meiosis processes ancestral to eukaryotes, with important divergences in each Kingdom.« less

Authors:
 [1];  [2];  [3];  [2];  [4];  [2];  [1];  [5];  [1];  [1]
  1. Stazione Zoologica Anton Dohrn, Naples (Italy)
  2. Ghent Univ., Ghent (Belgium); Flanders Institute for Biotechnology, Ghent (Belgium)
  3. Pontificia Univ. Catolica de Chile, Santiago (Chile); Sorbonne Univ., Roscoff (France)
  4. The Genome Analysis Centre (TGAC), Norwich (United Kingdom)
  5. Ghent Univ., Ghent (Belgium)
Publication Date:
Research Org.:
Univ. of California (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1241144
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 16; Journal Issue: 1; Journal ID: ISSN 1471-2164
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; meiosis; diatoms; sexual reproduction; SPO11; RAD51

Citation Formats

Patil, Shrikant, Moeys, Sara, von Dassow, Peter, Huysman, Marie J. J., Mapleson, Daniel, De Veylder, Lieven, Sanges, Remo, Vyverman, Wim, Montresor, Marina, and Ferrante, Maria Immacolata. Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta. United States: N. p., 2015. Web. doi:10.1186/s12864-015-1983-5.
Patil, Shrikant, Moeys, Sara, von Dassow, Peter, Huysman, Marie J. J., Mapleson, Daniel, De Veylder, Lieven, Sanges, Remo, Vyverman, Wim, Montresor, Marina, & Ferrante, Maria Immacolata. Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta. United States. doi:https://doi.org/10.1186/s12864-015-1983-5
Patil, Shrikant, Moeys, Sara, von Dassow, Peter, Huysman, Marie J. J., Mapleson, Daniel, De Veylder, Lieven, Sanges, Remo, Vyverman, Wim, Montresor, Marina, and Ferrante, Maria Immacolata. Sat . "Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta". United States. doi:https://doi.org/10.1186/s12864-015-1983-5. https://www.osti.gov/servlets/purl/1241144.
@article{osti_1241144,
title = {Identification of the meiotic toolkit in diatoms and exploration of meiosis-specific SPO11 and RAD51 homologs in the sexual species Pseudo-nitzschia multistriata and Seminavis robusta},
author = {Patil, Shrikant and Moeys, Sara and von Dassow, Peter and Huysman, Marie J. J. and Mapleson, Daniel and De Veylder, Lieven and Sanges, Remo and Vyverman, Wim and Montresor, Marina and Ferrante, Maria Immacolata},
abstractNote = {Sexual reproduction is an obligate phase in the life cycle of most eukaryotes. Meiosis varies among organisms, which is reflected by the variability of the gene set associated to the process. Diatoms are unicellular organisms that belong to the stramenopile clade and have unique life cycles that can include a sexual phase. The exploration of five diatom genomes and one diatom transcriptome led to the identification of 42 genes potentially involved in meiosis. While these include the majority of known meiosis-related genes, several meiosis-specific genes, including DMC1, could not be identified. Furthermore, phylogenetic analyses supported gene identification and revealed ancestral loss and recent expansion in the RAD51 family in diatoms. The two sexual species Pseudo-nitzschia multistriata and Seminavis robusta were used to explore the expression of meiosis-related genes: RAD21, SPO11-2, RAD51-A, RAD51-B and RAD51-C were upregulated during meiosis, whereas other paralogs in these families showed no differential expression patterns, suggesting that they may play a role during vegetative divisions. An almost identical toolkit is shared among Pseudo-nitzschia multiseries and Fragilariopsis cylindrus, as well as two species for which sex has not been observed, Phaeodactylum tricornutum and Thalassiosira pseudonana, suggesting that these two may retain a facultative sexual phase. Lastly, our results reveal the conserved meiotic toolkit in six diatom species and indicate that Stramenopiles share major modifications of canonical meiosis processes ancestral to eukaryotes, with important divergences in each Kingdom.},
doi = {10.1186/s12864-015-1983-5},
journal = {BMC Genomics},
number = 1,
volume = 16,
place = {United States},
year = {2015},
month = {11}
}

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Cited by: 9 works
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Figures / Tables:

Table 1 Table 1: Functional roles of meiotic genes searched in the diatom genomes

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    Works referencing / citing this record:

    Aberrant Sporogonic Development of Dmc1 (a Meiotic Recombinase) Deficient Plasmodium berghei Parasites
    journal, December 2012


    Nuclear inheritance and genetic exchange without meiosis in the binucleate parasite Giardia intestinalis
    journal, February 2012

    • Carpenter, M. L.; Assaf, Z. J.; Gourguechon, S.
    • Journal of Cell Science, Vol. 125, Issue 10
    • DOI: 10.1242/jcs.103879

    Genetic Analysis of Baker's Yeast Msh4-Msh5 Reveals a Threshold Crossover Level for Meiotic Viability
    journal, August 2010


    Molecular toolbox for studying diatom biology in Phaeodactylum tricornutum
    journal, December 2007


    REC, Drosophila MCM8, Drives Formation of Meiotic Crossovers
    journal, January 2005


    Bipartite stimulatory action of the Hop2-Mnd1 complex on the Rad51 recombinase
    journal, July 2007

    • Chi, P.; San Filippo, J.; Sehorn, M. G.
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