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Title: A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa

Abstract

Camelina sativa is an oilseed crop with great potential for biofuel production on marginal land. The seed oil from camelina has been converted to jet fuel and improved fuel efficiency in commercial and military test flights. Hydrogenation-derived renewable diesel from camelina is environmentally superior to that from canola due to lower agricultural inputs, and the seed meal is FDA approved for animal consumption. However, relatively low yield makes its farming less profitable. Our study is aimed at increasing camelina seed yield by reducing carbon loss from photorespiration via a photorespiratory bypass. Genes encoding three enzymes of the Escherichia coli glycolate catabolic pathway were introduced: glycolate dehydrogenase (GDH), glyoxylate carboxyligase (GCL) and tartronic semialdehyde reductase (TSR). These enzymes compete for the photorespiratory substrate, glycolate, convert it to glycerate within the chloroplasts, and reduce photorespiration. As a by-product of the reaction, CO2 is released in the chloroplast, which increases photosynthesis. Camelina plants were transformed with either partial bypass (GDH), or full bypass (GDH, GCL and TSR) genes. Furthermore, transgenic plants were evaluated for physiological and metabolic traits.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. North Carolina State Univ., Raleigh, NC (United States)
Publication Date:
Research Org.:
North Carolina State Univ., Raleigh, NC (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1239952
Grant/Contract Number:  
AR0000207
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Camelina; seed yield; biofuel; photorespiratory bypass; photosynthesis

Citation Formats

Dalal, Jyoti, Lopez, Harry, Vasani, Naresh B., Hu, Zhaohui, Swift, Jennifer E., Yalamanchili, Roopa, Dvora, Mia, Lin, Xiuli, Xie, Deyu, Qu, Rongda, and Sederoff, Heike W. A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa. United States: N. p., 2015. Web. doi:10.1186/s13068-015-0357-1.
Dalal, Jyoti, Lopez, Harry, Vasani, Naresh B., Hu, Zhaohui, Swift, Jennifer E., Yalamanchili, Roopa, Dvora, Mia, Lin, Xiuli, Xie, Deyu, Qu, Rongda, & Sederoff, Heike W. A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa. United States. doi:10.1186/s13068-015-0357-1.
Dalal, Jyoti, Lopez, Harry, Vasani, Naresh B., Hu, Zhaohui, Swift, Jennifer E., Yalamanchili, Roopa, Dvora, Mia, Lin, Xiuli, Xie, Deyu, Qu, Rongda, and Sederoff, Heike W. Thu . "A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa". United States. doi:10.1186/s13068-015-0357-1. https://www.osti.gov/servlets/purl/1239952.
@article{osti_1239952,
title = {A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa},
author = {Dalal, Jyoti and Lopez, Harry and Vasani, Naresh B. and Hu, Zhaohui and Swift, Jennifer E. and Yalamanchili, Roopa and Dvora, Mia and Lin, Xiuli and Xie, Deyu and Qu, Rongda and Sederoff, Heike W.},
abstractNote = {Camelina sativa is an oilseed crop with great potential for biofuel production on marginal land. The seed oil from camelina has been converted to jet fuel and improved fuel efficiency in commercial and military test flights. Hydrogenation-derived renewable diesel from camelina is environmentally superior to that from canola due to lower agricultural inputs, and the seed meal is FDA approved for animal consumption. However, relatively low yield makes its farming less profitable. Our study is aimed at increasing camelina seed yield by reducing carbon loss from photorespiration via a photorespiratory bypass. Genes encoding three enzymes of the Escherichia coli glycolate catabolic pathway were introduced: glycolate dehydrogenase (GDH), glyoxylate carboxyligase (GCL) and tartronic semialdehyde reductase (TSR). These enzymes compete for the photorespiratory substrate, glycolate, convert it to glycerate within the chloroplasts, and reduce photorespiration. As a by-product of the reaction, CO2 is released in the chloroplast, which increases photosynthesis. Camelina plants were transformed with either partial bypass (GDH), or full bypass (GDH, GCL and TSR) genes. Furthermore, transgenic plants were evaluated for physiological and metabolic traits.},
doi = {10.1186/s13068-015-0357-1},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 8,
place = {United States},
year = {2015},
month = {10}
}

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Cited by: 14 works
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Works referenced in this record:

Elevated CO2 concentration affects leaf photosynthesis-nitrogen relationships in Pinus taeda over nine years in FACE
journal, April 2008


Does Chloroplast Size Influence Photosynthetic Nitrogen Use Efficiency?
journal, April 2013


Metabolic Fluxes in an Illuminated Arabidopsis Rosette
journal, February 2013

  • Szecowka, Marek; Heise, Robert; Tohge, Takayuki
  • The Plant Cell, Vol. 25, Issue 2
  • DOI: 10.1105/tpc.112.106989

De novo assembly and characterization of Camelina sativa transcriptome by paired-end sequencing
journal, January 2013


Strong Expression and Conserved Regulation of ACT2 in Arabidopsis thaliana and Physcomitrella patens
journal, January 2010

  • An, Yong-Qiang Charles; Meagher, Richard B.
  • Plant Molecular Biology Reporter, Vol. 28, Issue 3
  • DOI: 10.1007/s11105-009-0171-7

Combinatorial modification of multiple lignin traits in trees through multigene cotransformation
journal, March 2003

  • Li, L.; Zhou, Y.; Cheng, X.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 8, p. 4939-4944
  • DOI: 10.1073/pnas.0831166100

Does the photosynthetic light-acclimation need change in leaf anatomy?
journal, April 2003


Nitrate assimilation in plant shoots depends on photorespiration
journal, July 2004

  • Rachmilevitch, S.; Cousins, A. B.; Bloom, A. J.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 31
  • DOI: 10.1073/pnas.0404388101

A possible role for the chloroplast pyruvate dehydrogenase complex in plant glycolate and glyoxylate metabolism
journal, November 2013


Sink regulation of photosynthesis
journal, July 2001


De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis
journal, July 2013

  • Haas, Brian J.; Papanicolaou, Alexie; Yassour, Moran
  • Nature Protocols, Vol. 8, Issue 8
  • DOI: 10.1038/nprot.2013.084

Fast and accurate short read alignment with Burrows-Wheeler transform
journal, May 2009


Technoeconomic analysis of jet fuel production from hydrolysis, decarboxylation, and reforming of camelina oil
journal, April 2015


Carbon Dioxide Enrichment Inhibits Nitrate Assimilation in Wheat and Arabidopsis
journal, May 2010


Fitting photosynthetic carbon dioxide response curves for C 3 leaves
journal, September 2007


Mass spectral characterization of picolinyl and methyl ester derivatives of isomeric thia fatty acids
journal, October 1991

  • Christie, W. W.; Brechany, E. Y.; Lie Ken Jie, M. S. F.
  • Biological Mass Spectrometry, Vol. 20, Issue 10
  • DOI: 10.1002/bms.1200201007

Transgenic Introduction of a Glycolate Oxidative Cycle into A. thaliana Chloroplasts Leads to Growth Improvement
journal, January 2012

  • Maier, Alexandra; Fahnenstich, Holger; von Caemmerer, Susanne
  • Frontiers in Plant Science, Vol. 3
  • DOI: 10.3389/fpls.2012.00038

How does photorespiration modulate leaf amino acid contents? A dual approach through modelling and metabolite analysis
journal, July 2002


Elevated CO 2 Induces Biochemical and Ultrastructural Changes in Leaves of the C 4 Cereal Sorghum
journal, July 2000

  • Watling, Jenny R.; Press, Malcolm C.; Quick, W. Paul
  • Plant Physiology, Vol. 123, Issue 3
  • DOI: 10.1104/pp.123.3.1143

The Benefits of Photorespiratory Bypasses: How Can They Work?
journal, December 2014

  • Xin, Chang-Peng; Tholen, Danny; Devloo, Vincent
  • Plant Physiology, Vol. 167, Issue 2
  • DOI: 10.1104/pp.114.248013

Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research
journal, August 2005


Camelina oil as a fuel for diesel transport engines
journal, May 2003


The Effect of Glyoxylate on Photosynthesis and Photorespiration by Isolated Soybean Mesophyll Cells
journal, May 1980


Photorespiration protects C3 plants from photooxidation
journal, December 1996


Glyoxylate Transamination in Intact Leaf Peroxisomes
journal, May 1984

  • Yu, Charles; Liang, Zheng; Huang, Anthony H. C.
  • Plant Physiology, Vol. 75, Issue 1
  • DOI: 10.1104/pp.75.1.7

Elevated CO2 and plant structure: a review
journal, October 1999


A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants
journal, September 2015


NIH Image to ImageJ: 25 years of image analysis
journal, June 2012

  • Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W.
  • Nature Methods, Vol. 9, Issue 7
  • DOI: 10.1038/nmeth.2089

Oil content of Arabidopsis seeds: The influence of seed anatomy, light and plant-to-plant variation
journal, May 2006


Sink feedback regulation of photosynthesis in vines: measurements and a model
journal, December 2001

  • Quereix, Anne; Dewar, Roderick C.; Gaudillere, Jean‐Pierre
  • Journal of Experimental Botany, Vol. 52, Issue 365
  • DOI: 10.1093/jexbot/52.365.2313

Glycolate oxidoreductase in Escherichia coli
journal, May 1972


Functional regions of the cauliflower mosaic virus 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity
journal, July 1987

  • Ow, D. W.; Jacobs, J. D.; Howell, S. H.
  • Proceedings of the National Academy of Sciences, Vol. 84, Issue 14
  • DOI: 10.1073/pnas.84.14.4870

The relationship of leaf photosynthetic traits - V cmax and J max - to leaf nitrogen, leaf phosphorus, and specific leaf area: a meta-analysis and modeling study
journal, July 2014

  • Walker, Anthony P.; Beckerman, Andrew P.; Gu, Lianhong
  • Ecology and Evolution, Vol. 4, Issue 16
  • DOI: 10.1002/ece3.1173

Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice
journal, March 2009

  • Xu, Huawei; Zhang, Jianjun; Zeng, Jiwu
  • Journal of Experimental Botany, Vol. 60, Issue 6
  • DOI: 10.1093/jxb/erp056

Toward Arabidopsis thaliana hydrophilic metabolome: assessment of extraction methods and quantitative 1H NMR
journal, September 2010


Pyruvate Dehydrogenase Complex from Higher Plant Mitochondria and Proplastids
journal, May 1977

  • Reid, E. Ellen; Thompson, Paul; Lyttle, C. Richard
  • Plant Physiology, Vol. 59, Issue 5
  • DOI: 10.1104/pp.59.5.842

Chloroplastic photorespiratory bypass increases photosynthesis and biomass production in Arabidopsis thaliana
journal, April 2007

  • Kebeish, Rashad; Niessen, Markus; Thiruveedhi, Krishnaveni
  • Nature Biotechnology, Vol. 25, Issue 5
  • DOI: 10.1038/nbt1299

Chloroplast and cytoplasmic enzymes II. Pea leaf triose phosphate isomerases
journal, April 1971


edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
journal, November 2009


Arabidopsis HT1 kinase controls stomatal movements in response to CO2
journal, March 2006

  • Hashimoto, Mimi; Negi, Juntaro; Young, Jared
  • Nature Cell Biology, Vol. 8, Issue 4
  • DOI: 10.1038/ncb1387

Subcellular volumes and metabolite concentrations in barley leaves
journal, August 1993

  • Winter, Heike; Robinson, DavidG.; Heldt, HansWalter
  • Planta, Vol. 191, Issue 2
  • DOI: 10.1007/BF00199748

Camelina-derived jet fuel and diesel: Sustainable advanced biofuels
journal, June 2010

  • Shonnard, David R.; Williams, Larry; Kalnes, Tom N.
  • Environmental Progress & Sustainable Energy, Vol. 29, Issue 3
  • DOI: 10.1002/ep.10461

Increasing photosynthesis by inhibiting photorespiration with glyoxylate
journal, June 1977


Predicting Nitrogen Fertilizer Needs for Rice in Arkansas Using Alkaline Hydrolyzable-Nitrogen
journal, January 2011

  • Roberts, Tl.; Ross, Wj.; Norman, Rj.
  • Soil Science Society of America Journal, Vol. 75, Issue 3
  • DOI: 10.2136/sssaj2010.0145

A constitutive gene expression system derived from the tCUP cryptic promoter elements
journal, September 2002


Species variation in kinetic properties of ribulose 1,5-bisphosphate carboxylase/oxygenase
journal, December 1983


Glyoxylate and Glutamate Effects on Photosynthetic Carbon Metabolism in Isolated Chloroplasts and Mesophyll Cells of Spinach
journal, June 1983

  • Lawyer, Arthur L.; Cornwell, Karen L.; Gee, Sherry L.
  • Plant Physiology, Vol. 72, Issue 2
  • DOI: 10.1104/pp.72.2.420

Soluble sugars mediate sink feedback down-regulation of leaf photosynthesis in field-grown Coffea arabica
journal, April 2006


Evaluation of Camelina sativa oil as a feedstock for biodiesel production
journal, January 2005


Photorespiration
journal, January 2010

  • Peterhansel, Christoph; Horst, Ina; Niessen, Markus
  • The Arabidopsis Book, Vol. 8
  • DOI: 10.1199/tab.0130

Current methods for estimating the rate of photorespiration in leaves
journal, November 2012


Regulation of photosynthetic electron-transport in Phaseolus vulgaris L., as determined by room-temperature chlorophyll a fluorescence
journal, December 1988

  • Sharkey, Thomas D.; Berry, Joseph A.; Sage, Rowan F.
  • Planta, Vol. 176, Issue 3
  • DOI: 10.1007/BF00395423

Qualitative variation of Camelina sativa seed from different locations
journal, May 2003


Gateway-compatible vectors for plant functional genomics and proteomics
journal, February 2006


Glycolate and Glyoxylate Metabolism by Isolated Peroxisomes or Chloroplasts
journal, February 1969


Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation
journal, September 2007


Isotopically nonstationary 13 C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation
journal, November 2014

  • Ma, Fangfang; Jazmin, Lara J.; Young, Jamey D.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 47
  • DOI: 10.1073/pnas.1319485111

Meeting the Global Food Demand of the Future by Engineering Crop Photosynthesis and Yield Potential
journal, March 2015


High-contrast three-dimensional imaging of the Arabidopsis leaf enables the analysis of cell dimensions in the epidermis and mesophyll
journal, January 2010

  • Wuyts, Nathalie; Palauqui, Jean-Christophe; Conejero, Genevieve
  • Plant Methods, Vol. 6, Issue 1
  • DOI: 10.1186/1746-4811-6-17

Some compositional properties of camelina (camelina sativa L. Crantz) seeds and oils
journal, March 1995

  • Budin, John T.; Breene, William M.; Putnam, Daniel H.
  • Journal of the American Oil Chemists’ Society, Vol. 72, Issue 3
  • DOI: 10.1007/BF02541088

Biodiesel from alternative oilseed feedstocks: camelina and field pennycress
journal, March 2012


Appearance of glycolate and related products of photosynthesis outside of chloroplasts
journal, July 1962


The respiratory energy requirements involved in nocturnal carbohydrate export from starch-storing mature source leaves and their contribution to leaf dark respiration
journal, January 1995

  • Bouma, T. J.; De Visser, R.; Van Leeuwen, P. H.
  • Journal of Experimental Botany, Vol. 46, Issue 9
  • DOI: 10.1093/jxb/46.9.1185

Effects of Glyoxylate on Photosynthesis by Intact Chloroplasts
journal, June 1983

  • Mulligan, R. Michael; Wilson, Barbara; Tolbert, N. Edward
  • Plant Physiology, Vol. 72, Issue 2
  • DOI: 10.1104/pp.72.2.415

A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species
journal, June 1980

  • Farquhar, G. D.; von Caemmerer, S.; Berry, J. A.
  • Planta, Vol. 149, Issue 1
  • DOI: 10.1007/BF00386231

Respiratory Oxygen Uptake Is Not Decreased by an Instantaneous Elevation of [CO 2 ], But Is Increased with Long-Term Growth in the Field at Elevated [CO 2 ]
journal, December 2003

  • Davey, Phillip A.; Hunt, Stephen; Hymus, Graham J.
  • Plant Physiology, Vol. 134, Issue 1
  • DOI: 10.1104/pp.103.030569

The Relationship Between CO2 Transfer Conductance and Leaf Anatomy in Transgenic Tobacco With a Reduced Content of Rubisco
journal, January 1994

  • Evans, Jr; Caemmerer, Sv; Setchell, Ba
  • Functional Plant Biology, Vol. 21, Issue 4
  • DOI: 10.1071/PP9940475

Agronomic evaluation of camelina genotypes selected for seed quality characteristics
journal, October 2007


A Rapid Method of Total Lipid Extraction and Purification
journal, August 1959

  • Bligh, E. G.; Dyer, W. J.
  • Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 8
  • DOI: 10.1139/o59-099

The leaf anatomy of a broad-leaved evergreen allows an increase in leaf nitrogen content in winter
journal, July 2009


Arabidopsis thaliana overexpressing glycolate oxidase in chloroplasts: H 2 O 2 -induced changes in primary metabolic pathways
journal, December 2008

  • Fahnenstich, Holger; Flügge, Ulf-Ingo; Maurino, Verónica G.
  • Plant Signaling & Behavior, Vol. 3, Issue 12
  • DOI: 10.4161/psb.3.12.7040

Agroecosystems, Nitrogen-use Efficiency, and Nitrogen Management
journal, March 2002

  • Cassman, Kenneth G.; Dobermann, Achim; Walters, Daniel T.
  • AMBIO: A Journal of the Human Environment, Vol. 31, Issue 2
  • DOI: 10.1579/0044-7447-31.2.132

Genomic basis for stimulated respiration by plants growing under elevated carbon dioxide
journal, February 2009

  • Leakey, A. D. B.; Xu, F.; Gillespie, K. M.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 9
  • DOI: 10.1073/pnas.0810955106

TM4: A Free, Open-Source System for Microarray Data Management and Analysis
journal, February 2003

  • Saeed, A. I.; Sharov, V.; White, J.
  • BioTechniques, Vol. 34, Issue 2
  • DOI: 10.2144/03342mt01

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