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Title: A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa

Abstract

Camelina sativa is an oilseed crop with great potential for biofuel production on marginal land. The seed oil from camelina has been converted to jet fuel and improved fuel efficiency in commercial and military test flights. Hydrogenation-derived renewable diesel from camelina is environmentally superior to that from canola due to lower agricultural inputs, and the seed meal is FDA approved for animal consumption. However, relatively low yield makes its farming less profitable. Our study is aimed at increasing camelina seed yield by reducing carbon loss from photorespiration via a photorespiratory bypass. Genes encoding three enzymes of the Escherichia coli glycolate catabolic pathway were introduced: glycolate dehydrogenase (GDH), glyoxylate carboxyligase (GCL) and tartronic semialdehyde reductase (TSR). These enzymes compete for the photorespiratory substrate, glycolate, convert it to glycerate within the chloroplasts, and reduce photorespiration. As a by-product of the reaction, CO 2 is released in the chloroplast, which increases photosynthesis. Camelina plants were transformed with either partial bypass (GDH), or full bypass (GDH, GCL and TSR) genes. Furthermore, transgenic plants were evaluated for physiological and metabolic traits.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. North Carolina State Univ., Raleigh, NC (United States)
Publication Date:
Research Org.:
North Carolina State Univ., Raleigh, NC (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1239952
Grant/Contract Number:  
AR0000207
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Camelina; seed yield; biofuel; photorespiratory bypass; photosynthesis

Citation Formats

Dalal, Jyoti, Lopez, Harry, Vasani, Naresh B., Hu, Zhaohui, Swift, Jennifer E., Yalamanchili, Roopa, Dvora, Mia, Lin, Xiuli, Xie, Deyu, Qu, Rongda, and Sederoff, Heike W. A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa. United States: N. p., 2015. Web. doi:10.1186/s13068-015-0357-1.
Dalal, Jyoti, Lopez, Harry, Vasani, Naresh B., Hu, Zhaohui, Swift, Jennifer E., Yalamanchili, Roopa, Dvora, Mia, Lin, Xiuli, Xie, Deyu, Qu, Rongda, & Sederoff, Heike W. A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa. United States. doi:10.1186/s13068-015-0357-1.
Dalal, Jyoti, Lopez, Harry, Vasani, Naresh B., Hu, Zhaohui, Swift, Jennifer E., Yalamanchili, Roopa, Dvora, Mia, Lin, Xiuli, Xie, Deyu, Qu, Rongda, and Sederoff, Heike W. Thu . "A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa". United States. doi:10.1186/s13068-015-0357-1. https://www.osti.gov/servlets/purl/1239952.
@article{osti_1239952,
title = {A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa},
author = {Dalal, Jyoti and Lopez, Harry and Vasani, Naresh B. and Hu, Zhaohui and Swift, Jennifer E. and Yalamanchili, Roopa and Dvora, Mia and Lin, Xiuli and Xie, Deyu and Qu, Rongda and Sederoff, Heike W.},
abstractNote = {Camelina sativa is an oilseed crop with great potential for biofuel production on marginal land. The seed oil from camelina has been converted to jet fuel and improved fuel efficiency in commercial and military test flights. Hydrogenation-derived renewable diesel from camelina is environmentally superior to that from canola due to lower agricultural inputs, and the seed meal is FDA approved for animal consumption. However, relatively low yield makes its farming less profitable. Our study is aimed at increasing camelina seed yield by reducing carbon loss from photorespiration via a photorespiratory bypass. Genes encoding three enzymes of the Escherichia coli glycolate catabolic pathway were introduced: glycolate dehydrogenase (GDH), glyoxylate carboxyligase (GCL) and tartronic semialdehyde reductase (TSR). These enzymes compete for the photorespiratory substrate, glycolate, convert it to glycerate within the chloroplasts, and reduce photorespiration. As a by-product of the reaction, CO2 is released in the chloroplast, which increases photosynthesis. Camelina plants were transformed with either partial bypass (GDH), or full bypass (GDH, GCL and TSR) genes. Furthermore, transgenic plants were evaluated for physiological and metabolic traits.},
doi = {10.1186/s13068-015-0357-1},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 8,
place = {United States},
year = {2015},
month = {10}
}

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Works referenced in this record:

Combinatorial modification of multiple lignin traits in trees through multigene cotransformation
journal, March 2003

  • Li, L.; Zhou, Y.; Cheng, X.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 8, p. 4939-4944
  • DOI: 10.1073/pnas.0831166100