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Title: Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic alaska

Abstract

We examined patterns in soil microbial community composition across a successional gradient of drained lake basins in the Arctic Coastal Plain. Analysis of 16S rRNA gene sequences revealed that methanogens closely related to Candidatus ‘Methanoflorens stordalenmirensis’ were the dominant archaea, comprising >50% of the total archaea at most sites, with particularly high levels in the oldest basins and in the top 57 cm of soil (active and transition layers). Bacterial community composition was more diverse, with lineages from OP11, Actinobacteria, Bacteroidetes, and Proteobacteria found in high relative abundance across all sites. Notably, microbial composition appeared to converge in the active layer, but transition and permafrost layer communities across the sites were significantly different to one another. Microbial biomass using fatty acid-based analysis indicated that the youngest basins had increased abundances of gram-positive bacteria and saprotrophic fungi at higher soil organic carbon levels, while the oldest basins displayed an increase in only the gram-positive bacteria. While this study showed differences in microbial populations across the sites relevant to basin age, the dominance of Candidatus ‘M. stordalenmirensis’ across the chronosequence indicates the potential for changes in local carbon cycling, depending on how these methanogens and associated microbial communities respond to warming temperatures.

Authors:
 [1];  [2];  [1];  [3];  [4];  [2];  [5];  [6]
  1. Cornell Univ., Ithaca, NY (United States)
  2. Univ. of Queensland (Australia)
  3. Univ. of Wisconsin, Madison, WI (United States)
  4. Yale Univ., New Haven, CT (United States)
  5. Univ. of Cincinnati, OH (United States)
  6. Technical Univ. of Muenchen (TRUM) (Germany)
Publication Date:
Research Org.:
Univ. of Arizona, Tucson, AZ (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1239801
Grant/Contract Number:  
SC0004632
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 5; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Kao-Kniffin, J., Woodcroft, B. J., Carver, S. M., Bockheim, J. G., Handelsman, J., Tyson, G. W., Hinkel, K. M., and Mueller, C. W. Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic alaska. United States: N. p., 2015. Web. https://doi.org/10.1038/srep18165.
Kao-Kniffin, J., Woodcroft, B. J., Carver, S. M., Bockheim, J. G., Handelsman, J., Tyson, G. W., Hinkel, K. M., & Mueller, C. W. Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic alaska. United States. https://doi.org/10.1038/srep18165
Kao-Kniffin, J., Woodcroft, B. J., Carver, S. M., Bockheim, J. G., Handelsman, J., Tyson, G. W., Hinkel, K. M., and Mueller, C. W. Fri . "Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic alaska". United States. https://doi.org/10.1038/srep18165. https://www.osti.gov/servlets/purl/1239801.
@article{osti_1239801,
title = {Archaeal and bacterial communities across a chronosequence of drained lake basins in arctic alaska},
author = {Kao-Kniffin, J. and Woodcroft, B. J. and Carver, S. M. and Bockheim, J. G. and Handelsman, J. and Tyson, G. W. and Hinkel, K. M. and Mueller, C. W.},
abstractNote = {We examined patterns in soil microbial community composition across a successional gradient of drained lake basins in the Arctic Coastal Plain. Analysis of 16S rRNA gene sequences revealed that methanogens closely related to Candidatus ‘Methanoflorens stordalenmirensis’ were the dominant archaea, comprising >50% of the total archaea at most sites, with particularly high levels in the oldest basins and in the top 57 cm of soil (active and transition layers). Bacterial community composition was more diverse, with lineages from OP11, Actinobacteria, Bacteroidetes, and Proteobacteria found in high relative abundance across all sites. Notably, microbial composition appeared to converge in the active layer, but transition and permafrost layer communities across the sites were significantly different to one another. Microbial biomass using fatty acid-based analysis indicated that the youngest basins had increased abundances of gram-positive bacteria and saprotrophic fungi at higher soil organic carbon levels, while the oldest basins displayed an increase in only the gram-positive bacteria. While this study showed differences in microbial populations across the sites relevant to basin age, the dominance of Candidatus ‘M. stordalenmirensis’ across the chronosequence indicates the potential for changes in local carbon cycling, depending on how these methanogens and associated microbial communities respond to warming temperatures.},
doi = {10.1038/srep18165},
journal = {Scientific Reports},
number = ,
volume = 5,
place = {United States},
year = {2015},
month = {12}
}

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