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Title: ‘Candidatus Liberibacter asiaticus’, causal agent of citrus Huanglongbing, is reduced by treatment with Brassinosteroids

Huanglongbing (HLB) constitutes the most destructive disease of citrus worldwide, yet no established efficient management measures exist for it. Brassinosteroids, a family of plant steroidal compounds, are essential for plant growth, development and stress tolerance. As a possible control strategy for HLB, epibrassinolide was applied to as a foliar spray to citrus plants infected with the causal agent of HLB, ‘Candidatus Liberibacter asiaticus’. The bacterial titers were reduced after treatment with epibrassinolide under both greenhouse and field conditions but were stronger in the greenhouse. Known defense genes were induced in leaves by epibrassinolide. With the SuperSAGE technology combined with next generation sequencing, induction of genes known to be associated with defense response to bacteria and hormone transduction pathways were identified. Lastly, the results demonstrate that epibrassinolide may provide a useful tool for the management of HLB.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [1] ;  [3] ;  [4] ;  [4] ;  [3] ;  [3] ;  [3] ;  [1] ;  [1] ;  [1] ;  [1] ;  [5] ;  [6] ;  [1] ;  [3] more »;  [7] ;  [7] ;  [8] ;  [8] ;  [9] ;  [1] ;  [1] ;  [1] « less
  1. Center for Genetic Engineering and Biotechnology, Havana (Cuba)
  2. Univ. of Havana, Havana (Cuba)
  3. Inst. of Tropical Fruit Investigations, Havana (Cuba)
  4. Empresa Agroindustrial Victoria de Giron, Matanzas (Cuba)
  5. Iwate Biotechnology Research Center, Kitakami (Japan)
  6. Shinshu Univ., Ueda (Japan)
  7. National Inst. of Agriculture Science, Mayabeque (Cuba)
  8. Michigan State Univ., East Lansing, MI (United States)
  9. Tobacco Research Inst., Havana (Cuba)
Publication Date:
Grant/Contract Number:
FG02-91ER20021
Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Research Org:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
citrus; DNA-binding proteins; transcription factors; polymerase chain reaction; leaves; plant biochemistry; gene expression; expressed sequence tags
OSTI Identifier:
1239246

Canales, Eduardo, Coll, Yamilet, Hernández, Ingrid, Portieles, Roxana, Rodríguez García, Mayra, López, Yunior, Aranguren, Miguel, Alonso, Eugenio, Delgado, Roger, Luis, Maritza, Batista, Lochy, Paredes, Camilo, Rodríguez, Meilyn, Pujol, Merardo, Ochagavia, María Elena, Falcón, Viviana, Terauchi, Ryohei, Matsumura, Hideo, Ayra-Pardo, Camilo, Llauger, Raixa, Pérez, María del Carmen, Núñez, Mirian, Borrusch, Melissa S., Walton, Jonathan D., Silva, Yussuan, Pimentel, Eulogio, Borroto, Carlos, and Borrás-Hidalgo, Orlando. ‘Candidatus Liberibacter asiaticus’, causal agent of citrus Huanglongbing, is reduced by treatment with Brassinosteroids. United States: N. p., Web. doi:10.1371/journal.pone.0146223.
Canales, Eduardo, Coll, Yamilet, Hernández, Ingrid, Portieles, Roxana, Rodríguez García, Mayra, López, Yunior, Aranguren, Miguel, Alonso, Eugenio, Delgado, Roger, Luis, Maritza, Batista, Lochy, Paredes, Camilo, Rodríguez, Meilyn, Pujol, Merardo, Ochagavia, María Elena, Falcón, Viviana, Terauchi, Ryohei, Matsumura, Hideo, Ayra-Pardo, Camilo, Llauger, Raixa, Pérez, María del Carmen, Núñez, Mirian, Borrusch, Melissa S., Walton, Jonathan D., Silva, Yussuan, Pimentel, Eulogio, Borroto, Carlos, & Borrás-Hidalgo, Orlando. ‘Candidatus Liberibacter asiaticus’, causal agent of citrus Huanglongbing, is reduced by treatment with Brassinosteroids. United States. doi:10.1371/journal.pone.0146223.
Canales, Eduardo, Coll, Yamilet, Hernández, Ingrid, Portieles, Roxana, Rodríguez García, Mayra, López, Yunior, Aranguren, Miguel, Alonso, Eugenio, Delgado, Roger, Luis, Maritza, Batista, Lochy, Paredes, Camilo, Rodríguez, Meilyn, Pujol, Merardo, Ochagavia, María Elena, Falcón, Viviana, Terauchi, Ryohei, Matsumura, Hideo, Ayra-Pardo, Camilo, Llauger, Raixa, Pérez, María del Carmen, Núñez, Mirian, Borrusch, Melissa S., Walton, Jonathan D., Silva, Yussuan, Pimentel, Eulogio, Borroto, Carlos, and Borrás-Hidalgo, Orlando. 2016. "‘Candidatus Liberibacter asiaticus’, causal agent of citrus Huanglongbing, is reduced by treatment with Brassinosteroids". United States. doi:10.1371/journal.pone.0146223. https://www.osti.gov/servlets/purl/1239246.
@article{osti_1239246,
title = {‘Candidatus Liberibacter asiaticus’, causal agent of citrus Huanglongbing, is reduced by treatment with Brassinosteroids},
author = {Canales, Eduardo and Coll, Yamilet and Hernández, Ingrid and Portieles, Roxana and Rodríguez García, Mayra and López, Yunior and Aranguren, Miguel and Alonso, Eugenio and Delgado, Roger and Luis, Maritza and Batista, Lochy and Paredes, Camilo and Rodríguez, Meilyn and Pujol, Merardo and Ochagavia, María Elena and Falcón, Viviana and Terauchi, Ryohei and Matsumura, Hideo and Ayra-Pardo, Camilo and Llauger, Raixa and Pérez, María del Carmen and Núñez, Mirian and Borrusch, Melissa S. and Walton, Jonathan D. and Silva, Yussuan and Pimentel, Eulogio and Borroto, Carlos and Borrás-Hidalgo, Orlando},
abstractNote = {Huanglongbing (HLB) constitutes the most destructive disease of citrus worldwide, yet no established efficient management measures exist for it. Brassinosteroids, a family of plant steroidal compounds, are essential for plant growth, development and stress tolerance. As a possible control strategy for HLB, epibrassinolide was applied to as a foliar spray to citrus plants infected with the causal agent of HLB, ‘Candidatus Liberibacter asiaticus’. The bacterial titers were reduced after treatment with epibrassinolide under both greenhouse and field conditions but were stronger in the greenhouse. Known defense genes were induced in leaves by epibrassinolide. With the SuperSAGE technology combined with next generation sequencing, induction of genes known to be associated with defense response to bacteria and hormone transduction pathways were identified. Lastly, the results demonstrate that epibrassinolide may provide a useful tool for the management of HLB.},
doi = {10.1371/journal.pone.0146223},
journal = {PLoS ONE},
number = 1,
volume = 11,
place = {United States},
year = {2016},
month = {1}
}