skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Assessment of Normal Variability in Peripheral Blood Gene Expression

Abstract

Peripheral blood is representative of many systemic processes and is an ideal sample for expression profiling of diseases that have no known or accessible lesion. Peripheral blood is a complex mixture of cell types and some differences in peripheral blood gene expression may reflect the timing of sample collection rather than an underlying disease process. For this reason, it is important to assess study design factors that may cause variability in gene expression not related to what is being analyzed. Variation in the gene expression of circulating peripheral blood mononuclear cells (PBMCs) from three healthy volunteers sampled three times one day each week for one month was examined for 1,176 genes printed on filter arrays. Less than 1% of the genes showed any variation in expression that was related to the time of collection, and none of the changes were noted in more than one individual. These results suggest that observed variation was due to experimental variability.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1239167
Resource Type:
Published Article
Journal Name:
Disease Markers
Additional Journal Information:
Journal Name: Disease Markers Journal Volume: 18 Journal Issue: 4; Journal ID: ISSN 0278-0240
Publisher:
Hindawi Publishing Corporation
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Campbell, Catherine, Vernon, Suzanne D., Karem, Kevin L., Nisenbaum, Rosane, and Unger, Elizabeth R. Assessment of Normal Variability in Peripheral Blood Gene Expression. Country unknown/Code not available: N. p., 2002. Web. doi:10.1155/2002/462465.
Campbell, Catherine, Vernon, Suzanne D., Karem, Kevin L., Nisenbaum, Rosane, & Unger, Elizabeth R. Assessment of Normal Variability in Peripheral Blood Gene Expression. Country unknown/Code not available. doi:10.1155/2002/462465.
Campbell, Catherine, Vernon, Suzanne D., Karem, Kevin L., Nisenbaum, Rosane, and Unger, Elizabeth R. Tue . "Assessment of Normal Variability in Peripheral Blood Gene Expression". Country unknown/Code not available. doi:10.1155/2002/462465.
@article{osti_1239167,
title = {Assessment of Normal Variability in Peripheral Blood Gene Expression},
author = {Campbell, Catherine and Vernon, Suzanne D. and Karem, Kevin L. and Nisenbaum, Rosane and Unger, Elizabeth R.},
abstractNote = {Peripheral blood is representative of many systemic processes and is an ideal sample for expression profiling of diseases that have no known or accessible lesion. Peripheral blood is a complex mixture of cell types and some differences in peripheral blood gene expression may reflect the timing of sample collection rather than an underlying disease process. For this reason, it is important to assess study design factors that may cause variability in gene expression not related to what is being analyzed. Variation in the gene expression of circulating peripheral blood mononuclear cells (PBMCs) from three healthy volunteers sampled three times one day each week for one month was examined for 1,176 genes printed on filter arrays. Less than 1% of the genes showed any variation in expression that was related to the time of collection, and none of the changes were noted in more than one individual. These results suggest that observed variation was due to experimental variability.},
doi = {10.1155/2002/462465},
journal = {Disease Markers},
number = 4,
volume = 18,
place = {Country unknown/Code not available},
year = {2002},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1155/2002/462465

Citation Metrics:
Cited by: 12 works
Citation information provided by
Web of Science

Save / Share: