Combining metabolic engineering and electrocatalysis: Application to the production of polyamides from sugar
Abstract
Here, biorefineries aim to convert biomass into a spectrum of products ranging from biofuels to specialty chemicals. To achieve economically sustainable conversion, it is crucial to streamline the catalytic and downstream processing steps. In this work, a route that combines bio- and electrocatalysis to convert glucose into bio-based unsaturated nylon-6,6 is reported. An engineered strain of Saccharomyces cerevisiae was used as the initial biocatalyst for the conversion of glucose into muconic acid, with the highest reported muconic acid titer of 559.5 mg L–1 in yeast. Without any separation, muconic acid was further electrocatalytically hydrogenated to 3-hexenedioic acid in 94 % yield despite the presence of biogenic impurities. Bio-based unsaturated nylon-6,6 (unsaturated polyamide-6,6) was finally obtained by polymerization of 3-hexenedioic acid with hexamethylenediamine.
- Authors:
-
- Iowa State Univ., Ames, IA (United States); NSF Engineering Research Center for Biorenewable Chemicals (CBiRC), Ames, IA (United States)
- Iowa State Univ., Ames, IA (United States); NSF Engineering Research Center for Biorenewable Chemicals (CBiRC), Ames, IA (United States); Ames Lab., Ames, IA (United States)
- Iowa State Univ., Ames, IA (United States)
- NSF Engineering Research Center for Biorenewable Chemicals (CBiRC), Ames, IA (United States)
- Publication Date:
- Research Org.:
- Ames Laboratory (AMES), Ames, IA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1239152
- Report Number(s):
- IS-J-8730
Journal ID: ISSN 1433-7851
- Grant/Contract Number:
- EEC0813570; EPSC1101284; AC02-07CH11358
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Angewandte Chemie (International Edition)
- Additional Journal Information:
- Journal Name: Angewandte Chemie (International Edition); Journal Volume: 55; Journal Issue: 7; Journal ID: ISSN 1433-7851
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Suastegui, Miguel, Matthiesen, John E., Carraher, Jack M., Hernandez, Nacu, Rodriguez Quiroz, Natalia, Okerlund, Adam, Cochran, Eric W., Shao, Zengyi, and Tessonnier, Jean -Philippe. Combining metabolic engineering and electrocatalysis: Application to the production of polyamides from sugar. United States: N. p., 2016.
Web. doi:10.1002/anie.201509653.
Suastegui, Miguel, Matthiesen, John E., Carraher, Jack M., Hernandez, Nacu, Rodriguez Quiroz, Natalia, Okerlund, Adam, Cochran, Eric W., Shao, Zengyi, & Tessonnier, Jean -Philippe. Combining metabolic engineering and electrocatalysis: Application to the production of polyamides from sugar. United States. https://doi.org/10.1002/anie.201509653
Suastegui, Miguel, Matthiesen, John E., Carraher, Jack M., Hernandez, Nacu, Rodriguez Quiroz, Natalia, Okerlund, Adam, Cochran, Eric W., Shao, Zengyi, and Tessonnier, Jean -Philippe. Thu .
"Combining metabolic engineering and electrocatalysis: Application to the production of polyamides from sugar". United States. https://doi.org/10.1002/anie.201509653. https://www.osti.gov/servlets/purl/1239152.
@article{osti_1239152,
title = {Combining metabolic engineering and electrocatalysis: Application to the production of polyamides from sugar},
author = {Suastegui, Miguel and Matthiesen, John E. and Carraher, Jack M. and Hernandez, Nacu and Rodriguez Quiroz, Natalia and Okerlund, Adam and Cochran, Eric W. and Shao, Zengyi and Tessonnier, Jean -Philippe},
abstractNote = {Here, biorefineries aim to convert biomass into a spectrum of products ranging from biofuels to specialty chemicals. To achieve economically sustainable conversion, it is crucial to streamline the catalytic and downstream processing steps. In this work, a route that combines bio- and electrocatalysis to convert glucose into bio-based unsaturated nylon-6,6 is reported. An engineered strain of Saccharomyces cerevisiae was used as the initial biocatalyst for the conversion of glucose into muconic acid, with the highest reported muconic acid titer of 559.5 mg L–1 in yeast. Without any separation, muconic acid was further electrocatalytically hydrogenated to 3-hexenedioic acid in 94 % yield despite the presence of biogenic impurities. Bio-based unsaturated nylon-6,6 (unsaturated polyamide-6,6) was finally obtained by polymerization of 3-hexenedioic acid with hexamethylenediamine.},
doi = {10.1002/anie.201509653},
journal = {Angewandte Chemie (International Edition)},
number = 7,
volume = 55,
place = {United States},
year = {Thu Jan 14 00:00:00 EST 2016},
month = {Thu Jan 14 00:00:00 EST 2016}
}
Web of Science
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