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Title: Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly

Abstract

A Chlamydomonas reinhardtii mutant lacking CGL71, a thylakoid membrane protein previously shown to be involved in photosystem I (PSI) accumulation, exhibited photosensitivity and highly reduced abundance of PSI under photoheterotrophic conditions. Remarkably, the PSI content of this mutant declined to nearly undetectable levels under dark, oxic conditions, demonstrating that reduced PSI accumulation in the mutant is not strictly the result of photodamage. Furthermore, PSI returns to nearly wild-type levels when the O 2 concentration in the medium is lowered. Overall, our results suggest that the accumulation of PSI in the mutant correlates with the redox state of the stroma rather than photodamage and that CGL71 functions under atmospheric O 2 conditions to allow stable assembly of PSI. These findings may reflect the history of the Earth’s atmosphere as it transitioned from anoxic to highly oxic (1–2 billion years ago), a change that required organisms to evolve mechanisms to assist in the assembly and stability of proteins or complexes with O 2 -sensitive cofactors.

Authors:
; ; ; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1238806
Grant/Contract Number:  
FG02-12ER16338
Resource Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 113 Journal Issue: 10; Journal ID: ISSN 0027-8424
Publisher:
Proceedings of the National Academy of Sciences
Country of Publication:
United States
Language:
English

Citation Formats

Heinnickel, Mark, Kim, Rick G., Wittkopp, Tyler M., Yang, Wenqiang, Walters, Karim A., Herbert, Stephen K., and Grossman, Arthur R. Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. United States: N. p., 2016. Web. doi:10.1073/pnas.1524040113.
Heinnickel, Mark, Kim, Rick G., Wittkopp, Tyler M., Yang, Wenqiang, Walters, Karim A., Herbert, Stephen K., & Grossman, Arthur R. Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. United States. doi:10.1073/pnas.1524040113.
Heinnickel, Mark, Kim, Rick G., Wittkopp, Tyler M., Yang, Wenqiang, Walters, Karim A., Herbert, Stephen K., and Grossman, Arthur R. Mon . "Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly". United States. doi:10.1073/pnas.1524040113.
@article{osti_1238806,
title = {Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly},
author = {Heinnickel, Mark and Kim, Rick G. and Wittkopp, Tyler M. and Yang, Wenqiang and Walters, Karim A. and Herbert, Stephen K. and Grossman, Arthur R.},
abstractNote = {A Chlamydomonas reinhardtii mutant lacking CGL71, a thylakoid membrane protein previously shown to be involved in photosystem I (PSI) accumulation, exhibited photosensitivity and highly reduced abundance of PSI under photoheterotrophic conditions. Remarkably, the PSI content of this mutant declined to nearly undetectable levels under dark, oxic conditions, demonstrating that reduced PSI accumulation in the mutant is not strictly the result of photodamage. Furthermore, PSI returns to nearly wild-type levels when the O 2 concentration in the medium is lowered. Overall, our results suggest that the accumulation of PSI in the mutant correlates with the redox state of the stroma rather than photodamage and that CGL71 functions under atmospheric O 2 conditions to allow stable assembly of PSI. These findings may reflect the history of the Earth’s atmosphere as it transitioned from anoxic to highly oxic (1–2 billion years ago), a change that required organisms to evolve mechanisms to assist in the assembly and stability of proteins or complexes with O 2 -sensitive cofactors.},
doi = {10.1073/pnas.1524040113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 10,
volume = 113,
place = {United States},
year = {2016},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1073/pnas.1524040113

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Cited by: 7 works
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