First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer
Abstract
Improved understanding of the relationship among structure, dynamics, and function for the enzyme phenylalanine hydroxylase (PAH) can lead to needed new therapies for phenylketonuria, the most common inborn error of amino acid metabolism. PAH is a multidomain homo-multimeric protein whose conformation and multimerization properties respond to allosteric activation by the substrate phenylalanine (Phe); the allosteric regulation is necessary to maintain Phe below neurotoxic levels. A recently introduced model for allosteric regulation of PAH involves major domain motions and architecturally distinct PAH tetramers [Jaffe EK, Stith L, Lawrence SH, Andrake M, Dunbrack RL, Jr (2013) Arch Biochem Biophys 530(2):73–82]. Herein, we present, to our knowledge, the first X-ray crystal structure for a full-length mammalian (rat) PAH in an autoinhibited conformation. Chromatographic isolation of a monodisperse tetrameric PAH, in the absence of Phe, facilitated determination of the 2.9 Å crystal structure. The structure of full-length PAH supersedes a composite homology model that had been used extensively to rationalize phenylketonuria genotype–phenotype relationships. Small-angle X-ray scattering (SAXS) confirms that this tetramer, which dominates in the absence of Phe, is different from a Phe-stabilized allosterically activated PAH tetramer. The lack of structural detail for activated PAH remains a barrier to complete understanding of phenylketonuria genotype–phenotypemore »
- Authors:
- Publication Date:
- Research Org.:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Cancer Institute Comprehensive Cancer Center; National Center for Research Resources of the National Inst. of Health
- OSTI Identifier:
- 1238204
- Alternate Identifier(s):
- OSTI ID: 1354369
- Report Number(s):
- BNL-112885-2016-JA
Journal ID: ISSN 0027-8424
- Grant/Contract Number:
- AC02-98CH10886; SC00112704; P41GM103473; P41RR012408; P30CA006927
- Resource Type:
- Published Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 113 Journal Issue: 9; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; phenylalanine hydroxylase; phenylketonuria; X-ray crystallography; small-angle X-ray scattering; allosteric regulation
Citation Formats
Arturo, Emilia C., Gupta, Kushol, Héroux, Annie, Stith, Linda, Cross, Penelope J., Parker, Emily J., Loll, Patrick J., and Jaffe, Eileen K. First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer. United States: N. p., 2016.
Web. doi:10.1073/pnas.1516967113.
Arturo, Emilia C., Gupta, Kushol, Héroux, Annie, Stith, Linda, Cross, Penelope J., Parker, Emily J., Loll, Patrick J., & Jaffe, Eileen K. First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer. United States. https://doi.org/10.1073/pnas.1516967113
Arturo, Emilia C., Gupta, Kushol, Héroux, Annie, Stith, Linda, Cross, Penelope J., Parker, Emily J., Loll, Patrick J., and Jaffe, Eileen K. Tue .
"First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer". United States. https://doi.org/10.1073/pnas.1516967113.
@article{osti_1238204,
title = {First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer},
author = {Arturo, Emilia C. and Gupta, Kushol and Héroux, Annie and Stith, Linda and Cross, Penelope J. and Parker, Emily J. and Loll, Patrick J. and Jaffe, Eileen K.},
abstractNote = {Improved understanding of the relationship among structure, dynamics, and function for the enzyme phenylalanine hydroxylase (PAH) can lead to needed new therapies for phenylketonuria, the most common inborn error of amino acid metabolism. PAH is a multidomain homo-multimeric protein whose conformation and multimerization properties respond to allosteric activation by the substrate phenylalanine (Phe); the allosteric regulation is necessary to maintain Phe below neurotoxic levels. A recently introduced model for allosteric regulation of PAH involves major domain motions and architecturally distinct PAH tetramers [Jaffe EK, Stith L, Lawrence SH, Andrake M, Dunbrack RL, Jr (2013) Arch Biochem Biophys 530(2):73–82]. Herein, we present, to our knowledge, the first X-ray crystal structure for a full-length mammalian (rat) PAH in an autoinhibited conformation. Chromatographic isolation of a monodisperse tetrameric PAH, in the absence of Phe, facilitated determination of the 2.9 Å crystal structure. The structure of full-length PAH supersedes a composite homology model that had been used extensively to rationalize phenylketonuria genotype–phenotype relationships. Small-angle X-ray scattering (SAXS) confirms that this tetramer, which dominates in the absence of Phe, is different from a Phe-stabilized allosterically activated PAH tetramer. The lack of structural detail for activated PAH remains a barrier to complete understanding of phenylketonuria genotype–phenotype relationships. Nevertheless, the use of SAXS and X-ray crystallography together to inspect PAH structure provides, to our knowledge, the first complete view of the enzyme in a tetrameric form that was not possible with prior partial crystal structures, and facilitates interpretation of a wealth of biochemical and structural data that was hitherto impossible to evaluate.},
doi = {10.1073/pnas.1516967113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 9,
volume = 113,
place = {United States},
year = {2016},
month = {2}
}
https://doi.org/10.1073/pnas.1516967113
Web of Science
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