Electron impact action spectroscopy of mass/charge selected macromolecular ions: Inner-shell excitation of ubiquitin protein

Journal Article · 10 February 2016 · Applied Physics Letters

DOI: https://doi.org/10.1063/1.4941798 · OSTI ID:1237899

Rankovic, Milos Lj. ^[1]; Giuliani, Alexandre ^[2]; Milosavljevic, Aleksandar R.  ^[3]

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1. Univ. of Belgrade, Belgrade (Serbia); Radiation Laboratory, University of Notre Dame
2. SOLEIL, l'Orme des Merisiers, Gif sur Yvette Cedex (France); INRA, UAR1008, CEPIA, Nantes (France)
3. Univ. of Belgrade, Belgrade (Serbia); Univ. of Notre Dame, Notre Dame, IN (United States)

In this study, we have performed inner-shell electron impact action spectroscopy of mass and charge selected macromolecular ions. For this purpose, we have coupled a focusing electron gun with a linear quadrupole ion trap mass spectrometer. This experiment represents a proof of principle that an energy-tunable electron beam can be used in combination with radio frequency traps as an activation method in tandem mass spectrometry (MS²) and allows performing action spectroscopy. Electron impact MS² spectra of multiply protonated ubiquitin protein ion have been recorded at incident electron energies around the carbon 1s excitation. Both MS² and single ionization energy dependence spectra are compared with literature data obtained using the soft X-ray activation conditions.

• Research Organization: Univ. of Notre Dame, Notre Dame, IN (United States)
• Sponsoring Organization: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
• Contributing Organization: ANR (France); MESTD (Republic of Serbia)
• Grant/Contract Number: FC02-04ER15533
• OSTI ID: 1237899
• Journal Information: Applied Physics Letters, Journal Name: Applied Physics Letters Journal Issue: 6 Vol. 108; ISSN APPLAB; ISSN 0003-6951
• Publisher: American Institute of Physics (AIP)Copyright Statement
• Country of Publication: United States
• Language: English