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Title: Electron impact action spectroscopy of mass/charge selected macromolecular ions: Inner-shell excitation of ubiquitin protein

In this study, we have performed inner-shell electron impact action spectroscopy of mass and charge selected macromolecular ions. For this purpose, we have coupled a focusing electron gun with a linear quadrupole ion trap mass spectrometer. This experiment represents a proof of principle that an energy-tunable electron beam can be used in combination with radio frequency traps as an activation method in tandem mass spectrometry (MS2) and allows performing action spectroscopy. Electron impact MS2 spectra of multiply protonated ubiquitin protein ion have been recorded at incident electron energies around the carbon 1s excitation. Both MS2 and single ionization energy dependence spectra are compared with literature data obtained using the soft X-ray activation conditions.
 [1] ;  [2] ; ORCiD logo [3]
  1. Univ. of Belgrade, Belgrade (Serbia)
  2. SOLEIL, l'Orme des Merisiers, Gif sur Yvette Cedex (France); INRA, UAR1008, CEPIA, Nantes (France)
  3. Univ. of Belgrade, Belgrade (Serbia); Univ. of Notre Dame, Notre Dame, IN (United States)
Publication Date:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Applied Physics Letters
Additional Journal Information:
Journal Volume: 108; Journal Issue: 6; Related Information: COST Action CM1204, COST Action CM1301; Journal ID: ISSN 0003-6951
American Institute of Physics (AIP)
Research Org:
Univ. of Notre Dame, Notre Dame, IN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Contributing Orgs:
ANR (France); MESTD (Republic of Serbia)
Country of Publication:
United States
74 ATOMIC AND MOLECULAR PHYSICS; action spectroscopy; RF ion trap; mass spectrometry; protein; inner shells; soft X-rays; electron spectroscopy; ion trapping; electron beams
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1237869