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Title: Electron impact action spectroscopy of mass/charge selected macromolecular ions: Inner-shell excitation of ubiquitin protein

In this study, we have performed inner-shell electron impact action spectroscopy of mass and charge selected macromolecular ions. For this purpose, we have coupled a focusing electron gun with a linear quadrupole ion trap mass spectrometer. This experiment represents a proof of principle that an energy-tunable electron beam can be used in combination with radio frequency traps as an activation method in tandem mass spectrometry (MS 2) and allows performing action spectroscopy. Electron impact MS 2 spectra of multiply protonated ubiquitin protein ion have been recorded at incident electron energies around the carbon 1s excitation. Both MS 2 and single ionization energy dependence spectra are compared with literature data obtained using the soft X-ray activation conditions.
Authors:
 [1] ;  [2] ; ORCiD logo [3]
  1. Univ. of Belgrade, Belgrade (Serbia)
  2. SOLEIL, l'Orme des Merisiers, Gif sur Yvette Cedex (France); INRA, UAR1008, CEPIA, Nantes (France)
  3. Univ. of Belgrade, Belgrade (Serbia); Univ. of Notre Dame, Notre Dame, IN (United States)
Publication Date:
Grant/Contract Number:
FC02-04ER15533
Type:
Accepted Manuscript
Journal Name:
Applied Physics Letters
Additional Journal Information:
Journal Volume: 108; Journal Issue: 6; Related Information: COST Action CM1204, COST Action CM1301; Journal ID: ISSN 0003-6951
Publisher:
American Institute of Physics (AIP)
Research Org:
Univ. of Notre Dame, Notre Dame, IN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Contributing Orgs:
ANR (France); MESTD (Republic of Serbia)
Country of Publication:
United States
Language:
English
Subject:
74 ATOMIC AND MOLECULAR PHYSICS; action spectroscopy; RF ion trap; mass spectrometry; protein; inner shells; soft X-rays; electron spectroscopy; ion trapping; electron beams
OSTI Identifier:
1237899
Alternate Identifier(s):
OSTI ID: 1237869