“Turn-On” Protein Fluorescence: In Situ Formation of Cyanine Dyes

Yapici, Ipek; Lee, Kin Sing Stephen; Berbasova, Tetyana; Nosrati, Meisam; Jia, Xiaofei; Vasileiou, Chrysoula; Wang, Wenjing; Santos, Elizabeth M.; Geiger, James H.; Borhan, Babak

doi:10.1021/ja506376j

Title: “Turn-On” Protein Fluorescence: In Situ Formation of Cyanine Dyes

Journal Article · 21 December 2014 · Journal of the American Chemical Society

DOI: https://doi.org/10.1021/ja506376j · OSTI ID:1237768

Yapici, Ipek ^[1]; Lee, Kin Sing Stephen ^[1]; Berbasova, Tetyana ^[1]; Nosrati, Meisam ^[1]; Jia, Xiaofei ^[1]; Vasileiou, Chrysoula ^[1]; Wang, Wenjing ^[1]; Santos, Elizabeth M. ^[1]; Geiger, James H. ^[1]; Borhan, Babak ^[1]

Michigan State Univ., East Lansing, MI (United States). Dept. of Chemistry

Protein reengineering of cellular retinoic acid binding protein II (CRABPII) has yielded a genetically addressable system, capable of binding a profluorophoric chromophore that results in fluorescent protein/chromophore complexes. These complexes exhibit far-red emission, with high quantum efficiencies and brightness and also exhibit excellent pH stability spanning the range of 2$$-$$11. In the course of this study, it became evident that single mutations of L121E and R59W were most effective in improving the fluorescent characteristics of CRABPII mutants as well as the kinetics of complex formation. The readily crystallizable nature of these proteins was invaluable to provide clues for the observed spectroscopic behavior that results from single mutation of key residues.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: W-31109-ENG-38

OSTI ID:: 1237768

Journal Information:: Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 3 Vol. 137; ISSN 0002-7863

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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