Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor

Maize, Kimberly M.; Kurbanov, Elbek K.; Johnson, Rodney L.; Amin, Elizabeth Ambrose; Finzel, Barry C.

doi:10.1016/j.febslet.2015.11.005

Title: Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor

Abstract

The Bacillus anthracis lethal factor (LF) is one component of a tripartite exotoxin partly responsible for persistent anthrax cytotoxicity after initial bacterial infection. Inhibitors of the zinc metalloproteinase have been investigated as potential therapeutic agents, but LF is a challenging target because inhibitors lack sufficient selectivity or possess poor pharmaceutical properties. Furthermore, these structural studies reveal an alternate conformation of the enzyme, induced upon binding of specific inhibitors, that opens a previously unobserved deep pocket termed S1'* which might afford new opportunities to design selective inhibitors that target this subsite.

Authors:

Maize, Kimberly M. ^[1]; Kurbanov, Elbek K. ^[1]; Johnson, Rodney L. ^[1]; Amin, Elizabeth Ambrose ^[1]; Finzel, Barry C. ^[1]

Univ. of Minnesota, Minneapolis, MN (United States)

Publication Date:: Wed Nov 11 00:00:00 EST 2015

Research Org.:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Org.:: National Institutes of Health (NIH)

OSTI Identifier:: 1237749

Grant/Contract Number:: R01 AI083234

Resource Type:: Accepted Manuscript

Journal Name:: FEBS Letters

Additional Journal Information:: Journal Volume: 589; Journal Issue: 24PartB; Journal ID: ISSN 0014-5793

Publisher:: Federation of European Biochemical Societies

Country of Publication:: United States

Language:: ENGLISH

Subject:: 59 BASIC BIOLOGICAL SCIENCES; LF; lethal factor; EF; edema factor; PA; protective antigen; MMP; matrix metalloprotease; Anthrax; Ligand-induced; Conformational change; Structure-based drug design; Zinc hydrolase

Citation Formats


                    Maize, Kimberly M., Kurbanov, Elbek K., Johnson, Rodney L., Amin, Elizabeth Ambrose, and Finzel, Barry C. Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor.  United States: N. p., 2015. 
Web.  doi:10.1016/j.febslet.2015.11.005.

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                    Maize, Kimberly M., Kurbanov, Elbek K., Johnson, Rodney L., Amin, Elizabeth Ambrose, & Finzel, Barry C. Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor.  United States.  https://doi.org/10.1016/j.febslet.2015.11.005

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                    Maize, Kimberly M., Kurbanov, Elbek K., Johnson, Rodney L., Amin, Elizabeth Ambrose, and Finzel, Barry C. Wed .  
"Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor".  United States.  https://doi.org/10.1016/j.febslet.2015.11.005.  https://www.osti.gov/servlets/purl/1237749.

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@article{osti_1237749,

  title        = {Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor},

  author       = {Maize, Kimberly M. and Kurbanov, Elbek K. and Johnson, Rodney L. and Amin, Elizabeth Ambrose and Finzel, Barry C.},

  abstractNote = {The Bacillus anthracis lethal factor (LF) is one component of a tripartite exotoxin partly responsible for persistent anthrax cytotoxicity after initial bacterial infection. Inhibitors of the zinc metalloproteinase have been investigated as potential therapeutic agents, but LF is a challenging target because inhibitors lack sufficient selectivity or possess poor pharmaceutical properties. Furthermore, these structural studies reveal an alternate conformation of the enzyme, induced upon binding of specific inhibitors, that opens a previously unobserved deep pocket termed S1'* which might afford new opportunities to design selective inhibitors that target this subsite.},

  doi          = {10.1016/j.febslet.2015.11.005},

  journal      = {FEBS Letters},

  number       = 24PartB,

  volume       = 589,

  place        = {United States},

  year         = {Wed Nov 11 00:00:00 EST 2015},

  month        = {Wed Nov 11 00:00:00 EST 2015}

}

Copy to clipboard

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https://doi.org/10.1016/j.febslet.2015.11.005

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Title: Ligand-induced expansion of the S1' site in the anthrax toxin lethal factor

Abstract

Citation Formats

Thioamide Hydroxypyrothiones Supersede Amide Hydroxypyrothiones in Potency against Anthrax Lethal Factor journal, February 2009

A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins book, January 2009

Chelator Fragment Libraries for Targeting Metalloproteinases journal, February 2010

Overview of the CCP 4 suite and current developments journal, March 2011

Anthrax journal, September 1999

Antidotes to anthrax lethal factor intoxication. Part 3: Evaluation of core structures and further modifications to the C2-side chain journal, March 2012

The Effect of Adjunctive Low-Dose Doxycycline Therapy on Clinical Parameters and Gingival Crevicular Fluid Matrix Metalloproteinase-8 Levels in Chronic Periodontitis journal, January 2004

Structural Basis for the Highly Selective Inhibition of MMP-13 journal, February 2005

Coot model-building tools for molecular graphics journal, November 2004

Anthrax Toxin journal, November 2003

Anthrax lethal factor inhibition journal, May 2005

Identification of Novel Non-Hydroxamate Anthrax Toxin Lethal Factor Inhibitors by Topomeric Searching, Docking and Scoring, and in Vitro Screening journal, November 2009

REFMAC 5 for the refinement of macromolecular crystal structures journal, March 2011

Anthrax toxin lethal factor domain 3 is highly mobile and responsive to ligand binding journal, October 2014

Towards automated crystallographic structure refinement with phenix.refine journal, March 2012

Identification of small molecule inhibitors of anthrax lethal factor journal, December 2003

PHENIX: a comprehensive Python-based system for macromolecular structure solution journal, January 2010

Randomized Phase III Trial of Marimastat Versus Placebo in Patients With Metastatic Breast Cancer Who Have Responding or Stable Disease After First-Line Chemotherapy: Eastern Cooperative Oncology Group Trial E2196 journal, December 2004

Structural Insight into the Stereoselective Inhibition of MMP-8 by Enantiomeric Sulfonamide Phosphonates journal, January 2006

Extra Binding Region Induced by Non-Zinc Chelating Inhibitors into the S 1 ′ Subsite of Matrix Metalloproteinase 8 (MMP-8) † journal, February 2009

Targeting Matrix Metalloproteinases: Exploring the Dynamics of the S1′ Pocket in the Design of Selective, Small Molecule Inhibitors: Miniperspective journal, October 2014

Efficient synthetic inhibitors of anthrax lethal factor journal, June 2005

Data processing and analysis with the autoPROC toolbox journal, March 2011

Anthrax as a Potential Biological Warfare Agent journal, March 1998

The discovery of a potent and selective lethal factor inhibitor for adjunct therapy of anthrax infection journal, February 2006

The structural basis for substrate and inhibitor selectivity of the anthrax lethal factor journal, December 2003

Crystal structure of the anthrax lethal factor journal, November 2001

[20] Processing of X-ray diffraction data collected in oscillation mode book, January 1997

DNASU plasmid and PSI:Biology-Materials repositories: resources to accelerate biological research journal, November 2013

JLigand : a graphical tool for the CCP 4 template-restraint library journal, March 2012

Phaser crystallographic software journal, July 2007

The roles of anthrax toxin in pathogenesis journal, February 2004

Matrix metalloproteinase inhibitors as therapy for inflammatory and vascular diseases journal, June 2007

Thioamide Hydroxypyrothiones Supersede Amide Hydroxypyrothiones in Potency against Anthrax Lethal Factor
journal, February 2009

A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins
book, January 2009

Chelator Fragment Libraries for Targeting Metalloproteinases
journal, February 2010

Overview of the CCP 4 suite and current developments
journal, March 2011

Anthrax
journal, September 1999

Antidotes to anthrax lethal factor intoxication. Part 3: Evaluation of core structures and further modifications to the C2-side chain
journal, March 2012

The Effect of Adjunctive Low-Dose Doxycycline Therapy on Clinical Parameters and Gingival Crevicular Fluid Matrix Metalloproteinase-8 Levels in Chronic Periodontitis
journal, January 2004

Structural Basis for the Highly Selective Inhibition of MMP-13
journal, February 2005

Coot model-building tools for molecular graphics
journal, November 2004

Anthrax Toxin
journal, November 2003

Anthrax lethal factor inhibition
journal, May 2005

Identification of Novel Non-Hydroxamate Anthrax Toxin Lethal Factor Inhibitors by Topomeric Searching, Docking and Scoring, and in Vitro Screening
journal, November 2009

REFMAC 5 for the refinement of macromolecular crystal structures
journal, March 2011

Anthrax toxin lethal factor domain 3 is highly mobile and responsive to ligand binding
journal, October 2014

Towards automated crystallographic structure refinement with phenix.refine
journal, March 2012

Identification of small molecule inhibitors of anthrax lethal factor
journal, December 2003

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

Randomized Phase III Trial of Marimastat Versus Placebo in Patients With Metastatic Breast Cancer Who Have Responding or Stable Disease After First-Line Chemotherapy: Eastern Cooperative Oncology Group Trial E2196
journal, December 2004

Structural Insight into the Stereoselective Inhibition of MMP-8 by Enantiomeric Sulfonamide Phosphonates
journal, January 2006

Extra Binding Region Induced by Non-Zinc Chelating Inhibitors into the S ₁ ′ Subsite of Matrix Metalloproteinase 8 (MMP-8) ^†
journal, February 2009

Targeting Matrix Metalloproteinases: Exploring the Dynamics of the S1′ Pocket in the Design of Selective, Small Molecule Inhibitors: Miniperspective
journal, October 2014

Efficient synthetic inhibitors of anthrax lethal factor
journal, June 2005

Data processing and analysis with the autoPROC toolbox
journal, March 2011

Anthrax as a Potential Biological Warfare Agent
journal, March 1998

The discovery of a potent and selective lethal factor inhibitor for adjunct therapy of anthrax infection
journal, February 2006

The structural basis for substrate and inhibitor selectivity of the anthrax lethal factor
journal, December 2003

Crystal structure of the anthrax lethal factor
journal, November 2001

[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997

DNASU plasmid and PSI:Biology-Materials repositories: resources to accelerate biological research
journal, November 2013

JLigand : a graphical tool for the CCP 4 template-restraint library
journal, March 2012

Phaser crystallographic software
journal, July 2007

The roles of anthrax toxin in pathogenesis
journal, February 2004

Matrix metalloproteinase inhibitors as therapy for inflammatory and vascular diseases
journal, June 2007