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Title: Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine

Abstract

In this study, various protocell models have been constructed de novo with the bottom-up approach. Here we describe a silica-based protocell composed of a nanoporous amorphous silica core encapsulated within a lipid bilayer built by self-assembly that provides for independent definition of cell interior and the surface membrane. In this review, we will first describe the essential features of this architecture and then summarize the current development of silica-based protocells at both micro- and nanoscale with diverse functionalities. As the structure of the silica is relatively static, silica-core protocells do not have the ability to change shape, but their interior structure provides a highly crowded and, in some cases, authentic scaffold upon which biomolecular components and systems could be reconstituted. In basic research, the larger protocells based on precise silica replicas of cells could be developed into geometrically realistic bioreactor platforms to enable cellular functions like coupled biochemical reactions, while in translational research smaller protocells based on mesoporous silica nanoparticles are being developed for targeted nanomedicine. Ultimately we see two different motivations for protocell research and development: (1) to emulate life in order to understand it; and (2) to use biomimicry to engineer desired cellular interactions.

Authors:
 [1];  [1];  [2];  [3]
  1. Univ. of Illinois, Urbana, IL (United States)
  2. Univ. of California San Diego, La Jolla, CA (United States)
  3. Univ. of New Mexico, Albuquerque, NM (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
National Science Foundation (NSF), Arlington, VA (United States)
OSTI Identifier:
1237664
Report Number(s):
SAND-2015-5323J
Journal ID: ISSN 2075-1729; LBSIB7; PII: life5010214
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Accepted Manuscript
Journal Name:
Life
Additional Journal Information:
Journal Volume: 5; Journal Issue: 1; Journal ID: ISSN 2075-1729
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY; protocell; nanoporous silica; synthetic biology; supported lipid bilayer; FRET; nanomedicine; designs of life

Citation Formats

Sun, Jie, Jakobsson, Eric, Wang, Yingxiao, and Brinker, C. Jeffrey. Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine. United States: N. p., 2015. Web. doi:10.3390/life5010214.
Sun, Jie, Jakobsson, Eric, Wang, Yingxiao, & Brinker, C. Jeffrey. Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine. United States. doi:10.3390/life5010214.
Sun, Jie, Jakobsson, Eric, Wang, Yingxiao, and Brinker, C. Jeffrey. Mon . "Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine". United States. doi:10.3390/life5010214. https://www.osti.gov/servlets/purl/1237664.
@article{osti_1237664,
title = {Nanoporous silica-based protocells at multiple scales for designs of life and nanomedicine},
author = {Sun, Jie and Jakobsson, Eric and Wang, Yingxiao and Brinker, C. Jeffrey},
abstractNote = {In this study, various protocell models have been constructed de novo with the bottom-up approach. Here we describe a silica-based protocell composed of a nanoporous amorphous silica core encapsulated within a lipid bilayer built by self-assembly that provides for independent definition of cell interior and the surface membrane. In this review, we will first describe the essential features of this architecture and then summarize the current development of silica-based protocells at both micro- and nanoscale with diverse functionalities. As the structure of the silica is relatively static, silica-core protocells do not have the ability to change shape, but their interior structure provides a highly crowded and, in some cases, authentic scaffold upon which biomolecular components and systems could be reconstituted. In basic research, the larger protocells based on precise silica replicas of cells could be developed into geometrically realistic bioreactor platforms to enable cellular functions like coupled biochemical reactions, while in translational research smaller protocells based on mesoporous silica nanoparticles are being developed for targeted nanomedicine. Ultimately we see two different motivations for protocell research and development: (1) to emulate life in order to understand it; and (2) to use biomimicry to engineer desired cellular interactions.},
doi = {10.3390/life5010214},
journal = {Life},
number = 1,
volume = 5,
place = {United States},
year = {2015},
month = {1}
}

Journal Article:
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