Kinetics of temperature response of PEO-b-PNIPAM-b-PAA triblock terpolymer aggregates and of their complexes with lysozyme
Abstract
We present the kinetics of temperature response of a PEO-b-PNIPAM-b-PAA triblock terpolymer and of its complexes with lysozyme in aqueous solution. It is found that during the coil-to-globule transition of PNIPAM new bonds within the polymer aggregates are created, making the transition of the aggregates partially irreversible. This effect is also found for the protein loaded PEO-b-PNIPAM-b-PAA aggregates whereas in this case protein globules appear to enhance the formation of bonds, making the transition totally irreversible. The internal dynamics of both aggregates and complexes are “frozen” once the temperature is increased upon PINIPAM's LCST in water and remain so even when the temperature drops below LCST. As a result, we investigate the complexation kinetics of lysozyme and PEO-b-PNIPAM-b-PAA and observe that it occurs in two stages, one where protein globules adsorb on single pre-formed aggregates and one where protein globules cause inter-aggregate clustering.
- Authors:
-
- National Hellenic Research Foundation, Athens (Greece)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1237639
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Polymer
- Additional Journal Information:
- Journal Volume: 83; Journal Issue: C; Journal ID: ISSN 0032-3861
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; thermoresponsive polymers; protein complexation; kinetics
Citation Formats
Papagiannopoulos, Aristeidis, Meristoudi, Anastasia, Hong, Kunlun, and Pispas, Stergios. Kinetics of temperature response of PEO-b-PNIPAM-b-PAA triblock terpolymer aggregates and of their complexes with lysozyme. United States: N. p., 2015.
Web. doi:10.1016/j.polymer.2015.12.023.
Papagiannopoulos, Aristeidis, Meristoudi, Anastasia, Hong, Kunlun, & Pispas, Stergios. Kinetics of temperature response of PEO-b-PNIPAM-b-PAA triblock terpolymer aggregates and of their complexes with lysozyme. United States. https://doi.org/10.1016/j.polymer.2015.12.023
Papagiannopoulos, Aristeidis, Meristoudi, Anastasia, Hong, Kunlun, and Pispas, Stergios. Fri .
"Kinetics of temperature response of PEO-b-PNIPAM-b-PAA triblock terpolymer aggregates and of their complexes with lysozyme". United States. https://doi.org/10.1016/j.polymer.2015.12.023. https://www.osti.gov/servlets/purl/1237639.
@article{osti_1237639,
title = {Kinetics of temperature response of PEO-b-PNIPAM-b-PAA triblock terpolymer aggregates and of their complexes with lysozyme},
author = {Papagiannopoulos, Aristeidis and Meristoudi, Anastasia and Hong, Kunlun and Pispas, Stergios},
abstractNote = {We present the kinetics of temperature response of a PEO-b-PNIPAM-b-PAA triblock terpolymer and of its complexes with lysozyme in aqueous solution. It is found that during the coil-to-globule transition of PNIPAM new bonds within the polymer aggregates are created, making the transition of the aggregates partially irreversible. This effect is also found for the protein loaded PEO-b-PNIPAM-b-PAA aggregates whereas in this case protein globules appear to enhance the formation of bonds, making the transition totally irreversible. The internal dynamics of both aggregates and complexes are “frozen” once the temperature is increased upon PINIPAM's LCST in water and remain so even when the temperature drops below LCST. As a result, we investigate the complexation kinetics of lysozyme and PEO-b-PNIPAM-b-PAA and observe that it occurs in two stages, one where protein globules adsorb on single pre-formed aggregates and one where protein globules cause inter-aggregate clustering.},
doi = {10.1016/j.polymer.2015.12.023},
journal = {Polymer},
number = C,
volume = 83,
place = {United States},
year = {2015},
month = {12}
}
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Works referenced in this record:
Emerging applications of stimuli-responsive polymer materials
journal, January 2010
- Stuart, Martien A. Cohen; Huck, Wilhelm T. S.; Genzer, Jan
- Nature Materials, Vol. 9, Issue 2, p. 101-113
Structure and design of polymeric surfactant-based drug delivery systems
journal, June 2001
- Torchilin, Vladimir P.
- Journal of Controlled Release, Vol. 73, Issue 2-3
Spherical polyelectrolyte brushes
journal, October 2007
- Ballauff, M.
- Progress in Polymer Science, Vol. 32, Issue 10
Self-Cross-Linked Polymer Nanogels: A Versatile Nanoscopic Drug Delivery Platform
journal, December 2010
- Ryu, Ja-Hyoung; Chacko, Reuben T.; Jiwpanich, Siriporn
- Journal of the American Chemical Society, Vol. 132, Issue 48
Thermoresponsive hydrogels in biomedical applications
journal, January 2008
- Klouda, Leda; Mikos, Antonios G.
- European Journal of Pharmaceutics and Biopharmaceutics, Vol. 68, Issue 1
Hydrogels in drug delivery: Progress and challenges
journal, April 2008
- Hoare, Todd R.; Kohane, Daniel S.
- Polymer, Vol. 49, Issue 8
Reduction and temperature dual-responsive crosslinked polymersomes for targeted intracellular protein delivery
journal, January 2011
- Cheng, Ru; Meng, Fenghua; Ma, Shoubao
- Journal of Materials Chemistry, Vol. 21, Issue 47
Morphological transitions in aggregates of thermosensitive poly(ethylene oxide)- b -poly( N -isopropylacrylamide) block copolymers prepared via RAFT polymerization: Morphological Transitions in PEO- b -PNIPAM
journal, July 2009
- Zhao, Junpeng; Zhang, Guangzhao; Pispas, Stergios
- Journal of Polymer Science Part A: Polymer Chemistry, Vol. 47, Issue 16
Micellar and fractal aggregates formed by two triblock terpolymers with different arrangements of one charged, one neutral hydrophilic and one hydrophobic block
journal, April 2015
- Papagiannopoulos, Aristeidis; Karayianni, Maria; Mountrichas, Grigoris
- Polymer, Vol. 63
Light Scattering by Fractal Aggregates: A Review
journal, January 2001
- Sorensen, C. M.
- Aerosol Science and Technology, Vol. 35, Issue 2
Dynamic light scattering study of internal motions of polymer coils in dilute solution
journal, December 1991
- Chu, Benjamin; Wang, Zhulun; Yu, Jiqun
- Macromolecules, Vol. 24, Issue 26
Asymptotic behavior and long-range interactions in aqueous solutions of poly(ethylene oxide)
journal, October 1991
- Devanand, K.; Selser, J. C.
- Macromolecules, Vol. 24, Issue 22
Characterization of Poly(N-isopropylmethacrylamide) in Water
journal, December 1990
- Kubota, Kenji; Hamano, Kenzi; Kuwahara, Nobuhiro
- Polymer Journal, Vol. 22, Issue 12
Structure of Micelles of Poly( n -butyl acrylate)- block -poly(acrylic acid) Diblock Copolymers in Aqueous Solution
journal, June 2007
- Colombani, Olivier; Ruppel, Markus; Burkhardt, Markus
- Macromolecules, Vol. 40, Issue 12
Thermoresponsive transition of a PEO-b-PNIPAM copolymer: From hierarchical aggregates to well defined ellipsoidal vesicles
journal, November 2013
- Papagiannopoulos, Aristeidis; Zhao, Junpeng; Zhang, Guangzhao
- Polymer, Vol. 54, Issue 23
Thermoresponsive aggregation of PS–PNIPAM–PS triblock copolymer: A combined study of light scattering and small angle neutron scattering
journal, July 2014
- Papagiannopoulos, Aristeidis; Zhao, Junpeng; Zhang, Guangzhao
- European Polymer Journal, Vol. 56
Interchain Hydrogen-Bonding-Induced Association of Poly(acrylic acid)- graft -poly(ethylene oxide) in Water
journal, February 2010
- Hao, Jinkun; Yuan, Guangcui; He, Weidong
- Macromolecules, Vol. 43, Issue 4
Globule-to-Coil Transition of a Single Homopolymer Chain in Solution
journal, May 1998
- Wu, Chi; Wang, Xiaohui
- Physical Review Letters, Vol. 80, Issue 18
Kinetics of aggregation in micellar solutions of thermoresponsive triblock copolymers – influence of concentration, start and target temperatures
journal, January 2013
- Adelsberger, Joseph; Grillo, Isabelle; Kulkarni, Amit
- Soft Matter, Vol. 9, Issue 5
Kinetics of Collapse Transition and Cluster Formation in a Thermoresponsive Micellar Solution of P(S-b-NIPAM-b-S) Induced by a Temperature Jump
journal, January 2012
- Adelsberger, Joseph; Metwalli, Ezzeldin; Diethert, Alexander
- Macromolecular Rapid Communications, Vol. 33, Issue 3
On the hydrodynamic radius of fractal aggregates
journal, November 1987
- Van Saarloos, Wim
- Physica A: Statistical Mechanics and its Applications, Vol. 147, Issue 1-2
Light scattering studies on core–shell systems: determination of size parameters of sterically stabilized poly(methylmethacrylate) dispersions
journal, April 2000
- Hirzinger, B.; Helmstedt, M.; Stejskal, J.
- Polymer, Vol. 41, Issue 8
Structure formation of surfactants in concentrated sulphuric acid: a light scattering study
journal, September 1995
- M�ller, A.; Burchard, W.
- Colloid & Polymer Science, Vol. 273, Issue 9
Thermo-sensitive poly(N-vinylcaprolactam-co-acetoacetoxyethyl methacrylate) microgels: 1—synthesis and characterization
journal, December 2003
- Boyko, Volodymyr; Pich, Andrij; Lu, Yan
- Polymer, Vol. 44, Issue 26
Temperature-Controlled Assembly and Release from Polymer Vesicles of Poly(ethylene oxide)-block- poly(N-isopropylacrylamide)
journal, November 2006
- Qin, S.; Geng, Y.; Discher, D. E.
- Advanced Materials, Vol. 18, Issue 21
Reversibly crosslinked temperature-responsive nano-sized polymersomes: synthesis and triggered drug release
journal, January 2009
- Xu, Haifei; Meng, Fenghua; Zhong, Zhiyuan
- Journal of Materials Chemistry, Vol. 19, Issue 24
Spectral interpretation of thermally irreversible recovery of poly(N-isopropylacrylamide-co-acrylic acid) hydrogel
journal, January 2011
- Sun, Shengtong; Hu, Jun; Tang, Hui
- Physical Chemistry Chemical Physics, Vol. 13, Issue 11
Homopolymer Self-Assembly into Stable Nanoparticles: Concerted Action of Hydrophobic Association and Hydrogen Bonding in Thermoresponsive Poly(alkylacrylic acid)s
journal, February 2012
- Sedlák, Marián
- The Journal of Physical Chemistry B, Vol. 116, Issue 8
Volume phase transition in a nonionic gel
journal, December 1984
- Hirokawa, Yoshitsugu; Tanaka, Toyoichi
- The Journal of Chemical Physics, Vol. 81, Issue 12
Formation of an Intrapolymer Complex from Human Serum Albumin and Poly(ethylene glycol)
journal, February 1999
- Azegami, Shinji; Tsuboi, Atsushi; Izumi, Tsuyoshi
- Langmuir, Vol. 15, Issue 4
Charge Stoichiometry Inside Polyelectrolyte−Protein Complexes: A Direct SANS Measurement for the PSSNa−Lysozyme System
journal, December 2006
- Gummel, Jérémie; Boué, François; Demé, Bruno
- The Journal of Physical Chemistry B, Vol. 110, Issue 49
Starch Fractions as Examples for Nonrandomly Branched Macromolecules. 4. Angular Dependence in Dynamic Light Scattering
journal, November 1997
- Galinsky, Gabriela; Burchard, Walther
- Macromolecules, Vol. 30, Issue 22
Works referencing / citing this record:
Formation of complexes in aqueous solutions of amphiphilic triblock polyelectrolytes of different topologies and an oppositely charged protein
journal, January 2018
- Papagiannopoulos, Aristeidis; Karayianni, Maria; Pispas, Stergios
- Soft Matter, Vol. 14, Issue 15