Overcoming substrate limitations for improved production of ethylene in E. coli
Abstract
Ethylene is an important industrial compound for the production of a wide variety of plastics and chemicals. At present, ethylene production involves steam cracking of a fossil-based feedstock, representing the highest CO2-emitting process in the chemical industry. Biological ethylene production can be achieved via expression of a single protein, the ethylene-forming enzyme (EFE), found in some bacteria and fungi; it has the potential to provide a sustainable alternative to steam cracking, provided that significant increases in productivity can be achieved. A key barrier is determining factors that influence the availability of substrates for the EFE reaction in potential microbial hosts. In the presence of O2, EFE catalyzes ethylene formation from the substrates α-ketoglutarate (AKG) and arginine. The concentrations of AKG, a key TCA cycle intermediate, and arginine are tightly controlled by an intricate regulatory system that coordinates carbon and nitrogen metabolism. Thus, reliably predicting which genetic changes will ultimately lead to increased AKG and arginine availability is challenging.
- Authors:
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1618634
- Alternate Identifier(s):
- OSTI ID: 1236036
- Report Number(s):
- NREL/JA-2700-63804
Journal ID: ISSN 1754-6834; 3; PII: 413
- Grant/Contract Number:
- SC008812; AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ethylene; ethylene-forming enzyme; arginine; α-ketoglutarate; E. coli
Citation Formats
Lynch, Sean, Eckert, Carrie, Yu, Jianping, Gill, Ryan, and Maness, Pin-Ching. Overcoming substrate limitations for improved production of ethylene in E. coli. Netherlands: N. p., 2016.
Web. doi:10.1186/s13068-015-0413-x.
Lynch, Sean, Eckert, Carrie, Yu, Jianping, Gill, Ryan, & Maness, Pin-Ching. Overcoming substrate limitations for improved production of ethylene in E. coli. Netherlands. https://doi.org/10.1186/s13068-015-0413-x
Lynch, Sean, Eckert, Carrie, Yu, Jianping, Gill, Ryan, and Maness, Pin-Ching. Mon .
"Overcoming substrate limitations for improved production of ethylene in E. coli". Netherlands. https://doi.org/10.1186/s13068-015-0413-x.
@article{osti_1618634,
title = {Overcoming substrate limitations for improved production of ethylene in E. coli},
author = {Lynch, Sean and Eckert, Carrie and Yu, Jianping and Gill, Ryan and Maness, Pin-Ching},
abstractNote = {Ethylene is an important industrial compound for the production of a wide variety of plastics and chemicals. At present, ethylene production involves steam cracking of a fossil-based feedstock, representing the highest CO2-emitting process in the chemical industry. Biological ethylene production can be achieved via expression of a single protein, the ethylene-forming enzyme (EFE), found in some bacteria and fungi; it has the potential to provide a sustainable alternative to steam cracking, provided that significant increases in productivity can be achieved. A key barrier is determining factors that influence the availability of substrates for the EFE reaction in potential microbial hosts. In the presence of O2, EFE catalyzes ethylene formation from the substrates α-ketoglutarate (AKG) and arginine. The concentrations of AKG, a key TCA cycle intermediate, and arginine are tightly controlled by an intricate regulatory system that coordinates carbon and nitrogen metabolism. Thus, reliably predicting which genetic changes will ultimately lead to increased AKG and arginine availability is challenging.},
doi = {10.1186/s13068-015-0413-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {Netherlands},
year = {Mon Jan 04 00:00:00 EST 2016},
month = {Mon Jan 04 00:00:00 EST 2016}
}
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