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Title: High-density grids for efficient data collection from multiple crystals

Higher throughput methods to mount and collect data from multiple small and radiation-sensitive crystals are important to support challenging structural investigations using microfocus synchrotron beamlines. Furthermore, efficient sample-delivery methods are essential to carry out productive femtosecond crystallography experiments at X-ray free-electron laser (XFEL) sources such as the Linac Coherent Light Source (LCLS). To address these needs, a high-density sample grid useful as a scaffold for both crystal growth and diffraction data collection has been developed and utilized for efficient goniometer-based sample delivery at synchrotron and XFEL sources. A single grid contains 75 mounting ports and fits inside an SSRL cassette or uni-puck storage container. The use of grids with an SSRL cassette expands the cassette capacity up to 7200 samples. Grids may also be covered with a polymer film or sleeve for efficient room-temperature data collection from multiple samples. New automated routines have been incorporated into theBlu-Ice/DCSSexperimental control system to support grids, including semi-automated grid alignment, fully automated positioning of grid ports, rastering and automated data collection. Specialized tools have been developed to support crystallization experiments on grids, including a universal adaptor, which allows grids to be filled by commercial liquid-handling robots, as well as incubation chambers, which support vapor-diffusionmore » and lipidic cubic phase crystallization experiments. Experiments in which crystals were loaded into grids or grown on grids using liquid-handling robots and incubation chambers are described. As a result, crystals were screened at LCLS-XPP and SSRL BL12-2 at room temperature and cryogenic temperatures.« less
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  1. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  2. Univ. of Pittsburgh School of Medicine, Pittsburgh, PA (United States)
  3. Art Robbins Instruments, Sunnyvale, CA (United States)
  4. Stanford Univ., Stanford, CA (United States)
  5. Monash Univ., Melbourne, Victoria (Australia); Australian Synchrotron, Clayton, Melbourne, Victoria (Australia)
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  7. Univ. of California, San Francisco, CA (United States)
  8. Humboldt-Univ. zu Berlin, Berlin (Germany)
  9. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States)
  10. Stanford Univ. School of Medicine, Stanford, CA (United States)
  11. Monash Univ., Melbourne, Victoria (Australia)
Publication Date:
Grant/Contract Number:
AC02-76SF00515; AC02-05CH11231
Accepted Manuscript
Journal Name:
Acta Crystallographica. Section D. Structural Biology
Additional Journal Information:
Journal Volume: 72; Journal Issue: 1; Journal ID: ISSN 2059-7983
Research Org:
SLAC National Accelerator Lab., Menlo Park, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; XFELs; high-throughput crystallography; serial crystallography; sample delivery; automation for sample-exchange robots
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1379000