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Title: Exploiting algal NADPH oxidase for biophotovoltaic energy

Abstract

Photosynthetic microbes exhibit light-dependent electron export across the cell membrane, which can generate electricity in biological photovoltaic (BPV) devices. How electrons are exported remains to be determined; the identification of mechanisms would help selection or generation of photosynthetic microbes capable of enhanced electrical output. We show that plasma membrane NADPH oxidase activity is a significant component of light-dependent generation of electricity by the unicellular green alga Chlamydomonas reinhardtii. NADPH oxidases export electrons across the plasma membrane to form superoxide anion from oxygen. The C. reinhardtii mutant lacking the NADPH oxidase encoded by RBO1 is impaired in both extracellular superoxide anion production and current generation in a BPV device. Complementation with the wild-type gene restores both capacities, demonstrating the role of the enzyme in electron export. Monitoring light-dependent extracellular superoxide production with a colorimetric assay is shown to be an effective way of screening for electrogenic potential of candidate algal strains. Furthermore, the results show that algal NADPH oxidases are important for superoxide anion production and open avenues for optimizing the biological component of these devices.

Authors:
 [1];  [2];  [3];  [1];  [1];  [3];  [1];  [1]
  1. Univ. of Cambridge, Cambridge (United Kingdom)
  2. Univ. of Cambridge, Cambridge (United Kingdom); RIKEN Centre for Sustainable Resource Sciences, Kanagawa (Japan)
  3. Univ. of California, Los Angeles, CA (United States)
Publication Date:
Research Org.:
Univ. of Cambridge, Cambridge (United Kingdom); University of California, Los Angeles
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1234170
Alternate Identifier(s):
OSTI ID: 1234171; OSTI ID: 1363910; OSTI ID: 1438189
Grant/Contract Number:  
FC02-02ER63421
Resource Type:
Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 14; Journal Issue: 1; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; alga; biophotovoltaic; Chlamydomonas; energy; NADPH oxidase; Chlamydomonas, energy

Citation Formats

Anderson, Alexander, Laohavisit, Anuphon, Blaby, Ian K., Bombelli, Paolo, Howe, Christopher J., Merchant, Sabeeha S., Davies, Julia M., and Smith, Alison G. Exploiting algal NADPH oxidase for biophotovoltaic energy. United States: N. p., 2015. Web. doi:10.1111/pbi.12332.
Anderson, Alexander, Laohavisit, Anuphon, Blaby, Ian K., Bombelli, Paolo, Howe, Christopher J., Merchant, Sabeeha S., Davies, Julia M., & Smith, Alison G. Exploiting algal NADPH oxidase for biophotovoltaic energy. United States. doi:10.1111/pbi.12332.
Anderson, Alexander, Laohavisit, Anuphon, Blaby, Ian K., Bombelli, Paolo, Howe, Christopher J., Merchant, Sabeeha S., Davies, Julia M., and Smith, Alison G. Thu . "Exploiting algal NADPH oxidase for biophotovoltaic energy". United States. doi:10.1111/pbi.12332.
@article{osti_1234170,
title = {Exploiting algal NADPH oxidase for biophotovoltaic energy},
author = {Anderson, Alexander and Laohavisit, Anuphon and Blaby, Ian K. and Bombelli, Paolo and Howe, Christopher J. and Merchant, Sabeeha S. and Davies, Julia M. and Smith, Alison G.},
abstractNote = {Photosynthetic microbes exhibit light-dependent electron export across the cell membrane, which can generate electricity in biological photovoltaic (BPV) devices. How electrons are exported remains to be determined; the identification of mechanisms would help selection or generation of photosynthetic microbes capable of enhanced electrical output. We show that plasma membrane NADPH oxidase activity is a significant component of light-dependent generation of electricity by the unicellular green alga Chlamydomonas reinhardtii. NADPH oxidases export electrons across the plasma membrane to form superoxide anion from oxygen. The C. reinhardtii mutant lacking the NADPH oxidase encoded by RBO1 is impaired in both extracellular superoxide anion production and current generation in a BPV device. Complementation with the wild-type gene restores both capacities, demonstrating the role of the enzyme in electron export. Monitoring light-dependent extracellular superoxide production with a colorimetric assay is shown to be an effective way of screening for electrogenic potential of candidate algal strains. Furthermore, the results show that algal NADPH oxidases are important for superoxide anion production and open avenues for optimizing the biological component of these devices.},
doi = {10.1111/pbi.12332},
journal = {Plant Biotechnology Journal},
number = 1,
volume = 14,
place = {United States},
year = {2015},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/pbi.12332

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Cited by: 5 works
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