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Title: A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase

Abstract

Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism

Authors:
;  [1];  [2];  [2];  [1];
  1. Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, United States
  2. Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, United States
Publication Date:
Research Org.:
Pennsylvania State Univ., University Park, PA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1234035
Alternate Identifier(s):
OSTI ID: 1438007
Grant/Contract Number:  
FG02-95ER20198 MOD16; FG02-95ER20198
Resource Type:
Published Article
Journal Name:
Biochemistry
Additional Journal Information:
Journal Name: Biochemistry Journal Volume: 54 Journal Issue: 47; Journal ID: ISSN 0006-2960
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wei, Yifeng, Li, Bin, Prakash, Divya, Ferry, James G., Elliott, Sean J., and Stubbe, JoAnne. A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase. United States: N. p., 2015. Web. doi:10.1021/acs.biochem.5b01092.
Wei, Yifeng, Li, Bin, Prakash, Divya, Ferry, James G., Elliott, Sean J., & Stubbe, JoAnne. A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase. United States. https://doi.org/10.1021/acs.biochem.5b01092
Wei, Yifeng, Li, Bin, Prakash, Divya, Ferry, James G., Elliott, Sean J., and Stubbe, JoAnne. Thu . "A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase". United States. https://doi.org/10.1021/acs.biochem.5b01092.
@article{osti_1234035,
title = {A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase},
author = {Wei, Yifeng and Li, Bin and Prakash, Divya and Ferry, James G. and Elliott, Sean J. and Stubbe, JoAnne},
abstractNote = {Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism},
doi = {10.1021/acs.biochem.5b01092},
journal = {Biochemistry},
number = 47,
volume = 54,
place = {United States},
year = {2015},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1021/acs.biochem.5b01092

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Cited by: 3 works
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