Molecular-Scale Features that Govern the Effects of O-Glycosylation on a Carbohydrate-Binding Module
Abstract
The protein glycosylation is a ubiquitous post-translational modification in all kingdoms of life. Despite its importance in molecular and cellular biology, the molecular-level ramifications of O-glycosylation on biomolecular structure and function remain elusive. Here, we took a small model glycoprotein and changed the glycan structure and size, amino acid residues near the glycosylation site, and glycosidic linkage while monitoring any corresponding changes to physical stability and cellulose binding affinity. The results of this study reveal the collective importance of all the studied features in controlling the most pronounced effects of O-glycosylation in this system. This study suggests the possibility of designing proteins with multiple improved properties by simultaneously varying the structures of O-glycans and amino acids local to the glycosylation site.
- Authors:
-
- Univ. of Colorado, Boulder, CO (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
- OSTI Identifier:
- 1233680
- Report Number(s):
- NREL/JA-5100-65609
Journal ID: ISSN 2041-6520
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Chemical Science
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 12; Related Information: Chemical Science; Journal ID: ISSN 2041-6520
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; protein glycosylation; biomolecular structure
Citation Formats
Guan, Xiaoyang, Chaffey, Patrick K., Zeng, Chen, Greene, Eric R., Chen, Liqun, Drake, Matthew R., Chen, Claire, Groobman, Ari, Resch, Michael G., Himmel, Michael E., Beckham, Gregg T., and Tan, Zhongping. Molecular-Scale Features that Govern the Effects of O-Glycosylation on a Carbohydrate-Binding Module. United States: N. p., 2015.
Web. doi:10.1039/C5SC02636A.
Guan, Xiaoyang, Chaffey, Patrick K., Zeng, Chen, Greene, Eric R., Chen, Liqun, Drake, Matthew R., Chen, Claire, Groobman, Ari, Resch, Michael G., Himmel, Michael E., Beckham, Gregg T., & Tan, Zhongping. Molecular-Scale Features that Govern the Effects of O-Glycosylation on a Carbohydrate-Binding Module. United States. https://doi.org/10.1039/C5SC02636A
Guan, Xiaoyang, Chaffey, Patrick K., Zeng, Chen, Greene, Eric R., Chen, Liqun, Drake, Matthew R., Chen, Claire, Groobman, Ari, Resch, Michael G., Himmel, Michael E., Beckham, Gregg T., and Tan, Zhongping. Mon .
"Molecular-Scale Features that Govern the Effects of O-Glycosylation on a Carbohydrate-Binding Module". United States. https://doi.org/10.1039/C5SC02636A. https://www.osti.gov/servlets/purl/1233680.
@article{osti_1233680,
title = {Molecular-Scale Features that Govern the Effects of O-Glycosylation on a Carbohydrate-Binding Module},
author = {Guan, Xiaoyang and Chaffey, Patrick K. and Zeng, Chen and Greene, Eric R. and Chen, Liqun and Drake, Matthew R. and Chen, Claire and Groobman, Ari and Resch, Michael G. and Himmel, Michael E. and Beckham, Gregg T. and Tan, Zhongping},
abstractNote = {The protein glycosylation is a ubiquitous post-translational modification in all kingdoms of life. Despite its importance in molecular and cellular biology, the molecular-level ramifications of O-glycosylation on biomolecular structure and function remain elusive. Here, we took a small model glycoprotein and changed the glycan structure and size, amino acid residues near the glycosylation site, and glycosidic linkage while monitoring any corresponding changes to physical stability and cellulose binding affinity. The results of this study reveal the collective importance of all the studied features in controlling the most pronounced effects of O-glycosylation in this system. This study suggests the possibility of designing proteins with multiple improved properties by simultaneously varying the structures of O-glycans and amino acids local to the glycosylation site.},
doi = {10.1039/C5SC02636A},
journal = {Chemical Science},
number = 12,
volume = 6,
place = {United States},
year = {Mon Sep 21 00:00:00 EDT 2015},
month = {Mon Sep 21 00:00:00 EDT 2015}
}
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Works referencing / citing this record:
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