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Title: Visualization of Bacterial Microcompartment Facet Assembly Using High-Speed Atomic Force Microscopy

Bacterial microcompartments (BMCs) are proteinaceous organelles widespread among bacterial phyla. They compartmentalize enzymes within a selectively permeable shell and play important roles in CO 2 fixation, pathogenesis, and microbial ecology. Here, we combine X-ray crystallography and high-speed atomic force microscopy to characterize, at molecular resolution, the structure and dynamics of BMC shell facet assembly. Our results show that preformed hexamers assemble into uniformly oriented shell layers, a single hexamer thick. We also observe the dynamic process of shell facet assembly. Shell hexamers can dissociate from and incorporate into assembled sheets, indicating a flexible intermolecular interaction. Furthermore, we demonstrate that the self-assembly and dynamics of shell proteins are governed by specific contacts at the interfaces of shell proteins. Our study provides novel insights into the formation, interactions, and dynamics of BMC shell facets, which are essential for the design and engineering of self-assembled biological nanoreactors and scaffolds based on BMC architectures.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [4] ;  [5] ;  [2]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
  2. Univ. of Liverpool (United Kingdom). Inst. of Integrative Biology
  3. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.
  4. Univ. of Liverpool (United Kingdom). Dept. of Physics
  5. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab. Dept. of Biochemistry and Molecular Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Berkeley Synthetic Biology Inst., Berkeley, CA (United States)
Publication Date:
Grant/Contract Number:
AC02-05CH11231; 1R01AI114975-01; UF120411; RG130442; BB/M024202/1; BB/M012441/1
Type:
Published Article
Journal Name:
Nano Letters
Additional Journal Information:
Journal Volume: 16; Journal Issue: 3; Journal ID: ISSN 1530-6984
Publisher:
American Chemical Society
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Michigan State Univ., East Lansing, MI (United States); Univ. of Liverpool (United Kingdom)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Inst. of Health (NIH) (United States); Royal Society (United Kingdom); Biotechnology and Biological Sciences Research Council (BBSRC) (United Kingdom)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bacterial microcompartment; high-speed atomic force microscopy; protein dynamics; protein interaction; self-assembly
OSTI Identifier:
1229691
Alternate Identifier(s):
OSTI ID: 1414741